Single microcolony diffusion analysis in Pseudomonas aeruginosa biofilms

Sankaran, Jagadish; Tan, Nicholas J. H. J.; But, Ka Pui; Cohen, Yehuda; Rice, Scott A.; Wohland, Thorsten

doi:10.1038/s41522-019-0107-4

Cited by 45 publications

(48 citation statements)

References 69 publications

(82 reference statements)

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“…3-BP is a low-molecular weight compound (166 g/mol), while molecular weight of bismuth subsalicylate (BIS) is more than two times higher (361 g/mol). A positive correlation between low-molecular weight of antimicrobials and their ability to penetrate the biofilm matrix has been already demonstrated by other research teams [ 47 , 48 ]. Therefore, it can be also assumed that low-weight 3BP would display significantly higher penetration through agar (a disk-diffusion assay) and biofilm matrix (an antimicrobial assay against lawn biofilms) compared to BIS.…”

Section: Discussionmentioning

confidence: 57%

Potential of Bacterial Cellulose Chemisorbed with Anti-Metabolites, 3-Bromopyruvate or Sertraline, to Fight against Helicobacter pylori Lawn Biofilm

Krzyżek

Gościniak

Fijałkowski

et al. 2020

IJMS

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Helicobacter pylori is a bacterium known mainly of its ability to cause persistent inflammations of the human stomach, resulting in peptic ulcer diseases and gastric cancers. Continuous exposure of this bacterium to antibiotics has resulted in high detection of multidrug-resistant strains and difficulties in obtaining a therapeutic effect. The purpose of the present study was to determine the usability of bacterial cellulose (BC) chemisorbed with 3-bromopyruvate (3-BP) or sertraline (SER) to act against lawn H. pylori biofilms. The characterization of BC carriers was made using a N2 adsorption/desorption analysis, tensile strength test, and scanning electron microscopy (SEM) observations. Determination of an antimicrobial activity was performed using a modified disk-diffusion method and a self-designed method of testing antibacterial activity against biofilm microbial forms. In addition, bacterial morphology was checked by SEM. It was found that BC disks were characterized by a high cross-linking and shear/stretch resistance. Growth inhibition zones for BC disks chemisorbed with 2 mg of SER or 3-BP were equal to 26.5–27.5 mm and 27–30 mm, respectively. The viability of lawn biofilm H. pylori cells after a 4-h incubation with 2 mg SER or 3-BP chemisorbed on BC disks was ≥4 log lower, suggesting their antibacterial effect. SEM observations showed a number of morphostructural changes in H. pylori cells exposed to these substances. Concluding, SER and 3-BP chemisorbed on BC carriers presented a promising antibacterial activity against biofilm H. pylori cells in in vitro conditions.

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Section: Discussionmentioning

confidence: 57%

Potential of Bacterial Cellulose Chemisorbed with Anti-Metabolites, 3-Bromopyruvate or Sertraline, to Fight against Helicobacter pylori Lawn Biofilm

Krzyżek

Gościniak

Fijałkowski

et al. 2020

IJMS

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“…The biofilm architecture of microcolonies, presence of EPS and water channels are often seen as contributors to the slowdown of convective transport and limit diffusion. It has been suggested that microcolonies act as molecular sieves and a diffusion coefficient is negatively correlated to the size of the colony 90 .…”

Section: Diffusion and Gradient Formation Of Reactive Species In Biofmentioning

confidence: 99%

Interactions of plasma-activated water with biofilms: inactivation, dispersal effects and mechanisms of action

Mai‐Prochnow

Zhou

Zhang

et al. 2021

npj Biofilms Microbiomes

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143

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Biofilms have several characteristics that ensure their survival in a range of adverse environmental conditions, including high cell numbers, close cell proximity to allow easy genetic exchange (e.g., for resistance genes), cell communication and protection through the production of an exopolysaccharide matrix. Together, these characteristics make it difficult to kill undesirable biofilms, despite the many studies aimed at improving the removal of biofilms. An elimination method that is safe, easy to deliver in physically complex environments and not prone to microbial resistance is highly desired. Cold atmospheric plasma, a lightning-like state generated from air or other gases with a high voltage can be used to make plasma-activated water (PAW) that contains many active species and radicals that have antimicrobial activity. Recent studies have shown the potential for PAW to be used for biofilm elimination without causing the bacteria to develop significant resistance. However, the precise mode of action is still the subject of debate. This review discusses the formation of PAW generated species and their impacts on biofilms. A focus is placed on the diffusion of reactive species into biofilms, the formation of gradients and the resulting interaction with the biofilm matrix and specific biofilm components. Such an understanding will provide significant benefits for tackling the ubiquitous problem of biofilm contamination in food, water and medical areas.

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“…Single plane illumination microscopy fluorescence correlation spectroscopy (SPIM-FCS). The protocol used was as previously described (80). Briefly, SPIM-FCS was used to quantitate the mobility of molecules diffusing in 3D in the biofilm matrix of the P. aeruginosa biofilm.…”

Section: Methodsmentioning

confidence: 99%

Sex Steroids Induce Membrane Stress Responses and Virulence Properties in Pseudomonas aeruginosa

Vidaillac

Yong

Aschtgen

et al. 2020

mBio

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Estrogen, a major female sex steroid hormone, has been shown to promote the selection of mucoid Pseudomonas aeruginosa in the airways of patients with chronic respiratory diseases, including cystic fibrosis. This results in long-term persistence, poorer clinical outcomes, and limited therapeutic options. In this study, we demonstrate that at physiological concentrations, sex steroids, including testosterone and estriol, induce membrane stress responses in P. aeruginosa. This is characterized by increased virulence and consequent inflammation and release of proinflammatory outer membrane vesicles promoting in vivo persistence of the bacteria. The steroid-induced P. aeruginosa response correlates with the molecular polarity of the hormones and membrane fluidic properties of the bacteria. This novel mechanism of interaction between sex steroids and P. aeruginosa explicates the reported increased disease severity observed in females with cystic fibrosis and provides evidence for the therapeutic potential of the modulation of sex steroids to achieve better clinical outcomes in patients with hormone-responsive strains. IMPORTANCE Molecular mechanisms by which sex steroids interact with P. aeruginosa to modulate its virulence have yet to be reported. Our work provides the first characterization of a steroid-induced membrane stress mechanism promoting P. aeruginosa virulence, which includes the release of proinflammatory outer membrane vesicles, resulting in inflammation, host tissue damage, and reduced bacterial clearance. We further demonstrate that at nanomolar (physiological) concentrations, male and female sex steroids promote virulence in clinical strains of P. aeruginosa based on their dynamic membrane fluidic properties. This work provides, for the first-time, mechanistic insight to better understand and predict the P. aeruginosa related response to sex steroids and explain the interindividual patient variability observed in respiratory diseases such as cystic fibrosis that are complicated by gender differences and chronic P. aeruginosa infection.

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Single microcolony diffusion analysis in Pseudomonas aeruginosa biofilms

Cited by 45 publications

References 69 publications

Potential of Bacterial Cellulose Chemisorbed with Anti-Metabolites, 3-Bromopyruvate or Sertraline, to Fight against Helicobacter pylori Lawn Biofilm

Potential of Bacterial Cellulose Chemisorbed with Anti-Metabolites, 3-Bromopyruvate or Sertraline, to Fight against Helicobacter pylori Lawn Biofilm

Interactions of plasma-activated water with biofilms: inactivation, dispersal effects and mechanisms of action

Sex Steroids Induce Membrane Stress Responses and Virulence Properties in Pseudomonas aeruginosa

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