Single Molecule Analysis of Self-Assembly Supramolecular Oligomers in Solution

Abstract

The supramolecular oligomers play an important role because their preorganized precise structures lead to a well-defined structure−property relationship. Here, we employed an α-hemolysin nanopore as a single molecule tool to investigate the self-assembly supramolecular oligomers of bis(p-sulfonatocalix[4]arene) (bisSC4) and bis-(methylviologen) (bisMV 4+ ). The self-assembly of supramolecular oligomers could be dynamic monitored at single molecule level. The association constant between bisSC4 and bisMV 4+ was calculated based on current blockage frequencies at the single molecule level. This work provides a complementary method to characterize the self-assembly oligomers with low molecular weight in solution.

Ultra-low noise measurements of nanopore-based single molecular detection

“…Therefore, nanopore technique has wide applications in the field of biology and clinical diagnosis [11][12][13]. The nanopore system consists of a nanoscale pore, detection chambers, electrodes and a signal recording system [14,15]. During detection, nanopores form on a membrane with nanoscale thickness, which is the only connection between two detection chambers filled with electrolyte ( Fig.…”

Single‐Molecule Sensing with Nanopore Confinement: From Chemical Reactions to Biological Interactions

“…The process of the 1:1c omplex self-assemble into the 1:2complex was observed in real-time with the nanopore-based approach.Similarly,t he self-assembly process of as upramolecular oligomer formed by the host-guest interactionb etween bis(psulfonatocalix [4]arene) (bisSC4) and bis(methylviologen) (bisMV 4 + )w as also investigated with a-HL nanopore. [58] The supramolecular oligomers produce long-lived current blockades, making it possible to dynamically monitor the self-assembly process in host-guest interactions at the single-molecule level. This work may offer an alternative for characterizingt he self-assembly oligomers with low molecular weighti ns olution.…”

Nanopore sensing of γ‐cyclodextrin induced host‐guest interaction to reverse the binding of perfluorooctanoic acid to human serum albumin

“…Recently, due to their label-free analytical capability and singlemolecule level sensitivity, nanopores have been developed as alternative analytical tools for single molecules and their interactions [18][19][20], which could provide crucial information from observation and analysis of ionic current modulations caused by the interactions between target analytes and fluidic pores [21][22][23]. In addition, because CDs fit well inside most nanopores and present a hydrophobic cavity suitable for binding organic analytes [24,25], they have also been involved in nanopore analysis and are widely used as adapters for detecting organic molecules [26,27].…”