Single-molecule anatomy by atomic force microscopy and recognition imaging

Takahashi, Hirohide; Hizume, Kohji; Kumeta, Masahiro; Yoshimura, Shige H.

doi:10.1679/aohc.72.217

Cited by 6 publications

(5 citation statements)

References 46 publications

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“…In a previous report in the same population, after adjusting for other confounding factors in the multivariable analysis narrower rim area was significantly associated with lower CSFP or higher TLCPD. Our findings are similar to the report that primary changes in CSFP or TLCPD are not likely to lead to laminar deformation or to produce a "glaucomatous" optic neuropathy in which the lamina deforms [23,[48][49][50][51][52][53][54][55].…”

Section: Discussionsupporting

confidence: 90%

Determinants of maximum cup depth in non-glaucoma and primary open-angle glaucoma subjects: a population-based study

Zhang

Chen

et al. 2019

Eye

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Background/objectives To study the associations of intraocular pressure (IOP) and retinal vessel diameters: central retinal arteriolar equivalent (CRAE) and central retinal venular equivalent (CRVE) with the maximum cup depth (MCD) in subjects with and without POAG. Subjects/methods Eligible subjects from the Handan Eye Study. All participants underwent physical and comprehensive eye examinations. Univariable and multivariable linear regression models assessed the association between MCD and other parameters. Results Four thousand one hundred and ninety-four eligible nonglaucoma and 40 POAG subjects were analyzed. On univariable analysis, deeper MCD was significantly associated with younger age, male gender, lower systolic blood pressure (BP), higher IOP, higher estimated cerebro-spinal fluid pressure (ECSFP), lower estimated trans-laminal cribrosa pressure difference (ETLCPD), longer axial length, narrower CRAE, narrower CRVE, larger disc area (DA) and a lower prevalence of hypertension and diabetes. On multivariable analysis, significant independent determinants of MCD were larger DA (P < 0.001; beta: 0.042; B: 0.20; 95% CI: 0.19, 0.22), younger age (P < 0.001; beta: −0.09; B: −0.002; 95% CI: −0.003, −0.001), higher IOP (P < 0.01; beta: 0.040; B: 0.003; 95% CI: 0.001, 0.005), and narrower CRAE (P < 0.001; beta: −0.06; B: −0.001; 95% CI: −0.001, −0.0003). On adding ECSFP and ETLCPD to the model, MCD was associated with IOP but not with estimated CSFP and TLCPD. A 1 μm decrease in CRAE or 1 mmHg increase of IOP was associated with a 1 μm increase of MCD (P < 0.001) and 3 μm increase of MCD respectively (P = 0.009). Conclusions Narrow CRVE and higher IOP are associated with an increase in MCD.

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Section: Discussionsupporting

confidence: 90%

Determinants of maximum cup depth in non-glaucoma and primary open-angle glaucoma subjects: a population-based study

Zhang

Chen

et al. 2019

Eye

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“…The ability of recognition imaging to characterize interactions between molecules of various shapes is one of its advantages over other single-molecule analytical techniques. Recognition imaging also enables the mapping of intra-molecular regions, such as the ligand-binding domain of α-actinin 4 (Takahashi et al, 2009). Using recognition imaging with synaptotagmin and SNAREs, we have shown that synaptotagmin can bind to both syntaxin 1A and the syntaxin 1A-SNAP-25 complex in the absence of Ca 2+ .…”

Section: +mentioning

confidence: 98%

Distinguishing between the molecular interactions of synaptotagmin with SNAREs and with lipid bilayer using atomic force microscopy in recognition imaging mode

Takahashi

Henderson

Edwardson

2013

Archives of Histology and Cytology

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Summary. Synaptic vesicle fusion with the presynaptic plasma membrane is mediated by the soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) proteins. Assembly of a complex between a vesicle-associated membrane protein, VAMP (a v-SNARE), in the vesicle membrane and a syntaxin 1A-SNAP-25 (t-SNAREs) heterodimer in the target presynaptic membrane drives fusion. Additionally, s y n a p t o t a g m i n a c t s a s a C a 2 + -s e n s i t i v e a n d phosphatidylserine (PS)-dependent trigger protein to initiate fusion. To dissect out the interaction of synaptotagmin with the t-SNAREs, we used atomic force microscopy (AFM) in recognition imaging mode to study its binding to the syntaxin 1A-SNAP-25 complex integrated into lipid bilayers without PS. Using a synaptotagmin-functionalized AFM cantilever, we identified a Ca 2+ -independent binding signal between synaptotagmin and the syntaxin 1A-SNAP-25 complex at the single-molecule level. Within the nerve terminal, this Ca 2+ -independent interaction could potentially locate synaptotagmin close to the t-SNAREs in preparation for recognition of the incoming Ca 2+ signal.

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“…AFM-FS (Stroh et al, 2004;Hinterdorfer and Dufrene 2006;Tang et al, 2007;Dufrene and Hinterdorfer 2008;Takahashi et al, 2009) is crucial due to its wide range of applications (Mayyas et al, 2010). Other than determining the binding kinetics and interaction forces, AFM-FS has also been utilized to understand the unfolding and folding of nucleic acids with multiple repeated units and single proteins, as well as to study molecule-material interface and molecule-molecule interactions.…”

Section: Understanding the Binding Dynamics Between The Targeted Receptor And Drug Delivery Vehiclementioning

confidence: 99%

“…All these modes will be described in more details in the following sections of this review paper. "Recognition AFM" or recognition imaging has facilitated usage of AFM (Stroh et al, 2004;Ebner et al, 2005;Hinterdorfer and Dufrene 2006;Tang et al, 2007;Dufrene and Hinterdorfer 2008;Takahashi et al, 2009;Chtcheglova and Hinterdorfer 2018;Koehler et al, 2019) as biosensor and enhanced its performance as a "characterization and detection tool" of biomolecules. A magnetic cantilever, stimulated by an external magnetic field, is oscillated in liquid in topography and recognition imaging (TREC) as shown in Figures 2A-B (Ebner et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Biosensing, Characterization of Biosensors, and Improved Drug Delivery Approaches Using Atomic Force Microscopy: A Review

Sarkar

2022

Front. Nanotechnol.

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Since its invention, atomic force microscopy (AFM) has come forth as a powerful member of the “scanning probe microscopy” (SPM) family and an unparallel platform for high-resolution imaging and characterization for inorganic and organic samples, especially biomolecules, biosensors, proteins, DNA, and live cells. AFM characterizes any sample by measuring interaction force between the AFM cantilever tip (the probe) and the sample surface, and it is advantageous over other SPM and electron micron microscopy techniques as it can visualize and characterize samples in liquid, ambient air, and vacuum. Therefore, it permits visualization of three-dimensional surface profiles of biological specimens in the near-physiological environment without sacrificing their native structures and functions and without using laborious sample preparation protocols such as freeze-drying, staining, metal coating, staining, or labeling. Biosensors are devices comprising a biological or biologically extracted material (assimilated in a physicochemical transducer) that are utilized to yield electronic signal proportional to the specific analyte concentration. These devices utilize particular biochemical reactions moderated by isolated tissues, enzymes, organelles, and immune system for detecting chemical compounds via thermal, optical, or electrical signals. Other than performing high-resolution imaging and nanomechanical characterization (e.g., determining Young’s modulus, adhesion, and deformation) of biosensors, AFM cantilever (with a ligand functionalized tip) can be transformed into a biosensor (microcantilever-based biosensors) to probe interactions with a particular receptors of choice on live cells at a single-molecule level (using AFM-based single-molecule force spectroscopy techniques) and determine interaction forces and binding kinetics of ligand receptor interactions. Targeted drug delivery systems or vehicles composed of nanoparticles are crucial in novel therapeutics. These systems leverage the idea of targeted delivery of the drug to the desired locations to reduce side effects. AFM is becoming an extremely useful tool in figuring out the topographical and nanomechanical properties of these nanoparticles and other drug delivery carriers. AFM also helps determine binding probabilities and interaction forces of these drug delivery carriers with the targeted receptors and choose the better agent for drug delivery vehicle by introducing competitive binding. In this review, we summarize contributions made by us and other researchers so far that showcase AFM as biosensors, to characterize other sensors, to improve drug delivery approaches, and to discuss future possibilities.

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Single-molecule anatomy by atomic force microscopy and recognition imaging

Cited by 6 publications

References 46 publications

Determinants of maximum cup depth in non-glaucoma and primary open-angle glaucoma subjects: a population-based study

Determinants of maximum cup depth in non-glaucoma and primary open-angle glaucoma subjects: a population-based study

Distinguishing between the molecular interactions of synaptotagmin with SNAREs and with lipid bilayer using atomic force microscopy in recognition imaging mode

Biosensing, Characterization of Biosensors, and Improved Drug Delivery Approaches Using Atomic Force Microscopy: A Review

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