Single Molecule Binding of CD44 to Fibrin Versus P-selectin Predicts Their Distinct Shear-dependent Interactions in Cancer

Raman, Phrabha S.; Alves, Christina; Wirtz, Denis; Κωνσταντόπουλος, Κωνσταντίνος

doi:10.1016/j.bpj.2010.12.2820

Cited by 11 publications

(46 citation statements)

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“…S is the survival probability or the probability that the rupture has not occurred yet at time t , and t* is the time of rupture. This probability density function was fit to each experimental adhesion force distribution by probing fit parameters using Monte Carlo optimization methods424344.…”

Section: Methodsmentioning

confidence: 99%

Adhesion and fusion efficiencies of human immunodeficiency virus type 1 (HIV-1) surface proteins

Dobrowsky

Rabi

Nedellec

et al. 2013

Sci Rep

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In about half of patients infected with HIV-1 subtype B, viral populations shift from utilizing the transmembrane protein CCR5 to CXCR4, as well as or instead of CCR5, during late stage progression of the disease. How the relative adhesion efficiency and fusion competency of the viral Env proteins relate to infection during this transition is not well understood. Using a virus-cell fusion assay and live-cell single-molecule force spectroscopy, we compare the entry competency of viral clones to tensile strengths of the individual Env-receptor bonds of Env proteins obtained from a HIV-1 infected patient prior to and during coreceptor switching. The results suggest that the genetic determinants of viral entry were predominantly enriched in the C3, HR1 and CD regions rather than V3. Env proteins can better mediate entry into cells after coreceptor switch; this effective entry capacity does not correlate with the bond strengths between viral Env and cellular receptors.

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Section: Methodsmentioning

confidence: 99%

Adhesion and fusion efficiencies of human immunodeficiency virus type 1 (HIV-1) surface proteins

Dobrowsky

Rabi

Nedellec

et al. 2013

Sci Rep

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“…The research done by the Konstantopoulos group has shown that CD44 expressed on colon cancer cells is the main receptor for fibrin, and that the CD44v-fibrin interaction dominates at low shear rates because of the increased interaction time between the two molecules. 7,8, 131 In addition, fibrin has also been shown to serve as a bridging molecule between PMNs and CTCs, allowing for cancer cell-leukocyte aggregation. 179 A group of important glycosylated ligands consists of oligosaccharides that bear sialic acid, fucose, and sulfate at the tips of O-linked glycans.…”

Section: Metastatic Cascade: Adhesive Recruitment Of Cancer Cells Viamentioning

confidence: 99%

Glycomechanics of the Metastatic Cascade: Tumor Cell–Endothelial Cell Interactions in the Circulation

2011

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Hydrodynamic shear force plays an important role in the leukocyte adhesion cascade that involves the tethering and rolling of cells along the endothelial layer, their firm adhesion or arrest, and their extravasation or escape from the circulatory system by inducing passive deformation, or cell flattening, and microvilli stretching, as well as regulating the expression, distribution, and conformation of adhesion molecules on leukocytes and the endothelial layer. Similarly, the dissemination of circulating tumor cells (CTCs) from the primary tumor sites is believed to involve tethering, rolling, and firm adhesion steps before their eventual extravasation which leads to secondary tumor sites (metastasis). Of particular importance to both the leukocyte adhesion cascade and the extravasation of CTCs, glycoproteins are involved in all three steps (capture, rolling, and firm adhesion) and consist of a variety of important selectin ligands. This review article provides an overview of glycoprotein glycosylation associated with the abnormal glycan expression on cancer cell surfaces, where well-established and novel selectin ligands that are cancer related are discussed. An overview of computational approaches on the effects of fluid mechanical force on glycoprotein mediated cancer cell rolling and adhesion is presented with a highlight of recent flow-based and selectin-mediated cell capturing/enriching devices. Finally, as an important branch of the glycoprotein family, mucins, specifically MUC1, are discussed in the context of their aberrant expression on cancer cells and their role as cancer cell adhesion molecules. Since metastasis relies heavily on glycoprotein interactions in the bloodstream where the fluid shear stress highly regulates cell adhesion forces, it is important to study and understand the glycomechanics of all relevant glycoproteins (well-established and novel) as they relate to the metastatic cascade.

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“…2a). 37 These results showed that sFn elicited a higher percentage of binding events than both sFg and function-blocked sFn. Moreover, the binding of CD44 to sFg was similar to background levels, suggesting that sFg has a minimal role in the initial adhesions of tumor cells to the endothelium.…”

Section: Fibrin(ogen)-mediated Adhesions Of Melanoma Cells To the Endmentioning

confidence: 87%

“…2b). 37 However, the higher tensile strength of P-selectin-CD44 interactions in comparison to sFn-CD44 interactions suggests that P-selectin is crucial in initial adhesions, while sFn imparts stability to the bonds.…”

Section: Fibrin(ogen)-mediated Adhesions Of Melanoma Cells To the Endmentioning

confidence: 98%

“…The affinity of fibrin for tumor cell receptors has been demonstrated by Raman et al using single-molecule force spectroscopy. 18,37 In the study, a cantilever was coated with either sFn, functionally-blocked sFn, or sFg and brought into contact with lipid bilayers containing the CD44 colon carcinoma surface receptor (Fig. 2a).…”

Section: Fibrin(ogen)-mediated Adhesions Of Melanoma Cells To the Endmentioning

confidence: 99%

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Perspectives: Interplay Between Melanoma Regulated Fibrin and Receptor Mediated Adhesion Under Shear Flow

et al. 2014

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Metastasis is a complex process that ultimately results in secondary tumor formation, posing a threat to patient survival. However, the mechanisms by which circulating tumor cells adhere to the endothelium and escape from the vasculature are not fully understood. To bind to the endothelium, tumor cells must resist the shear forces induced by flow, largely through receptor-mediated cell-cell interactions. Tumor cells are able to manipulate the metastatic site microenvironment via thrombin and soluble fibrin (sFn) regulation, impacting both coagulation and cell adhesion processes. Thus, thrombosis may be recognized as a prognostic indicator of highly metastatic tumors. Herein, we describe some correlations between the metastatic potential of tumor cells and coagulation. This perspectives review focuses on the role of sFn in melanoma-endothelial adhesions under shear conditions. Specifically, sFn serves as a divalent ligand for a v b 3 , CD11b/CD18 (a m b 2 , Mac-1), and intercellular adhesion molecule-1 (ICAM-1) transmembrane receptors. The cross-linking role of sFn is examined in relation to host leukocytes, in particular, the polymorphonuclear neutrophil (PMN)-mediated adhesions of melanoma cells to the endothelium. These results have shed light on the significant interplay between melanoma cells, PMNs, and the endothelium under shear conditions, which is key in the struggle to better diagnose and treat melanoma metastases.

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Single Molecule Binding of CD44 to Fibrin Versus P-selectin Predicts Their Distinct Shear-dependent Interactions in Cancer

Cited by 11 publications

References 0 publications

Adhesion and fusion efficiencies of human immunodeficiency virus type 1 (HIV-1) surface proteins

Adhesion and fusion efficiencies of human immunodeficiency virus type 1 (HIV-1) surface proteins

Glycomechanics of the Metastatic Cascade: Tumor Cell–Endothelial Cell Interactions in the Circulation

Perspectives: Interplay Between Melanoma Regulated Fibrin and Receptor Mediated Adhesion Under Shear Flow

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