Single-molecule detection of chaperonin dynamics through polarization rotation modulation of CdSe QD luminescence imaging

Tani, Tadaaki; Oda, Masahiro; Araki, Daisuke; Miyashita, T.; Nakajima, Kyoko; Arita, Mayuno; Yohda, Masafumi

doi:10.1016/j.jlumin.2014.01.059

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…154 The measurement of the angular components of a tracked molecule using polarization modulation techniques could provide information about conformational changes and add rotational orientation inside a living cell as another dimension to SPT. 155,156 Similar to super-resolution one of the key factors for SPT measurements is the successful delivery of the conjugates into the cytoplasm, which can be problematic for QDs due to their large sizes or surface charges. Cellular delivery will be discussed in more detail in Section 4.1.…”

Section: Single-particle Trackingmentioning

confidence: 99%

Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

2015

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Semiconductor quantum dots (QDs) have become important fluorescent probes for in vitro and in vivo bioimaging research. Their nanoparticle surfaces for versatile bioconjugation, their adaptable photophysical properties for multiplexed detection, and their superior stability for longer investigation times are the main advantages of QDs compared to other fluorescence imaging agents. Here, we review the recent literature dealing with the design and application of QD-bioconjugates for advanced in vitro and in vivo imaging. After a short summary of QD preparation and their most important properties, different QD-based imaging applications will be discussed from the technological and the biological point of view, ranging from super-resolution microscopy and single-particle tracking over in vitro cell and tissue imaging to in vivo investigations. A substantial part of the review will focus on multifunctional applications, in which the QD fluorescence is combined with drug or gene delivery towards theranostic approaches or with complementary technologies for multimodal imaging. We also briefly discuss QD toxicity issues and give a short outlook on future directions of QD-based bioimaging.

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Section: Single-particle Trackingmentioning

confidence: 99%

Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

2015

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“…The first class is the combination of non-fluorescent biodegradable polymers and fluorescent agents. A variety of chromophores have been chosen as labels, including small organic dyes as rhodamine [13], metal-ligand complexes as [Ru(bpy) 3 ] 2+ [14], QDs made from II/VI and III/V semiconductors [15,16], carbon [17] and silicon nanoparticles [18], etc. From a physiological point of view, biocompatibility of the fluorescent materials is the first priority.…”

Section: Introductionmentioning

confidence: 99%

Visualization of in vivo degradation of aliphatic polyesters by a fluorescent dendritic star macromolecule

Duan¹,

Ma²,

Huang³

et al. 2015

Biomed. Mater.

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In tissue engineering, most polymeric scaffolds should degrade along with the formation of the new tissues. Therefore, it is necessary to look into the in vivo degradation of scaffolds. In this study, a fluorescent perylenediimide-cored (PDI-cored) dendritic star macromolecule bearing multiple amines (d-p48) was incorporated into biodegradable polyester nanofibrous scaffolds by eletrospinning as an indicator. The polyester/d-p48 blend nanofibers could emit strong red fluorescence when they were irradiated under exciting light. Initially, using slowly degradable polyester, poly(L-lactide) (PLLA)/d-p48 nanofibers were soaked in phosphate buffered saline for various lengths of time to determine the possible diffusing release of d-p48 macromolecule from nanofibers. The PLLA/d-p48 nanofibers were then implanted subcutaneously into mice and left for up to 2 weeks. In both cases, no undesirable release of the incorporated d-p48 macromolecule was detected, and the nanofibers were clearly visualized in vivo by fluorescence microscopy. Using a fast degradable polyester, poly(lactide-co-glycolide) (PLGA)/d-p48 nanofibers were electrospun and implanted subcutaneously to determine the possibility of monitoring in vivo degradation by fluorescence during 12 weeks. The results showed that the location and the contour of PLGA/d-p48 nanofibrous scaffolds could be clearly visualized using an animal fluorescent imaging system. The fluorescent intensities decreased gradually with the degradation of the scaffolds. No side effects on liver and kidney were found during the detection. This study indicates that the fluorescent PDI-cored dendritic star macromolecule can be used as a stable bioimaging indicator for biodegradable aliphatic polyesters in vivo.

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An ultrasensitive aptasensor for Ochratoxin A using hexagonal core/shell upconversion nanoparticles as luminophores

Dai

Duan

et al. 2017

Biosensors and Bioelectronics

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Single-molecule detection of chaperonin dynamics through polarization rotation modulation of CdSe QD luminescence imaging

Cited by 3 publications

References 15 publications

Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

Visualization of in vivo degradation of aliphatic polyesters by a fluorescent dendritic star macromolecule

An ultrasensitive aptasensor for Ochratoxin A using hexagonal core/shell upconversion nanoparticles as luminophores

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