“…Each data point was estimated from the reported longest residencesofpeptides.R elated references were shown in the right parentheses, where asterisk indicate the capability for identification of single AA difference. Compared to the results obtained with wild-type (WT) biological nanopores(open circles),[25,28,29,44,48,54,66,67,69,73,[75][76][77][78][79][80][81] translocation velocities of peptides have been remarkably reduced by designingmutant nanopores( red solid circles),[30,70,82,83] introducing dipolar-like peptide structures (orange solid triangles),[57,58,[84][85][86] and optimizing solution conditions, such as temperatures (blue solid diamonds)[87,88] and pH (violet solid pentagons) [89]. Ideal velocitiesinsuggested region were coloured in light blue.…”