2018
DOI: 10.1101/430371
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Single-molecule live cell imaging of Rep reveals the dynamic interplay between an accessory replicative helicase and the replisome

Abstract: DNA replication requires strategies to cope with nucleoprotein barriers that impair the efficient translocation of the replisome. Biochemical and genetic studies indicate accessory helicases play essential roles in continuity of replication in the presence of nucleoprotein barriers, but how they operate in the native cellular environment is unclear. With high-speed single-molecule microscopy we determine the dynamic patterns of localization of genomically-encoded fluorescent protein constructs of the bacterial… Show more

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Cited by 3 publications
(2 citation statements)
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“…Because TADR uses Rep helicase from E. coli, it may cause offtarget mutations in the chromosome, where Rep helicase normally participates in DNA replication (29). Consistent with this expectation, removal of the initiation sequence for CisA decreased mutagenesis only 4.2-fold (Fig.…”
Section: Maximizing On-target and Minimizing Off-target Mutagenesissupporting
confidence: 62%
“…Because TADR uses Rep helicase from E. coli, it may cause offtarget mutations in the chromosome, where Rep helicase normally participates in DNA replication (29). Consistent with this expectation, removal of the initiation sequence for CisA decreased mutagenesis only 4.2-fold (Fig.…”
Section: Maximizing On-target and Minimizing Off-target Mutagenesissupporting
confidence: 62%
“…Accessory replicative helicases are required in cells in order to facilitate the progression of the bacterial replication machinery called the replisome [36] through nucleoprotein complexes that would otherwise block replication [37][38][39][40][41][42][43]. Rep is the accessory replicative helicase in Escherichia coli bacteria that removes protein blocks to DNA replication bound to DNA as well as helping to restart the stalled replication machinery [44]. It contains four subdomains (1A, 2A, 1B and 2B), of which 2B can rotate around a hinge region allowing for a significant conformational change in the protein [27,28,[45][46][47][48][49][50] (see Figure 2a, b).…”
Section: Introductionmentioning
confidence: 99%