2019
DOI: 10.1016/j.cell.2019.05.029
|View full text |Cite
|
Sign up to set email alerts
|

Single-Molecule Nanoscopy Elucidates RNA Polymerase II Transcription at Single Genes in Live Cells

Abstract: Highlights d Real-time single-molecule detection in high-background, crowded cellular locales d Imaging and tracking Pol II at single genes during the transcription cycle d Organization, dynamics, and interdependencies of enhancer-bound regulatory factors d Dissection of enhancer mechanisms at the endogenous

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

11
177
1

Year Published

2019
2019
2023
2023

Publication Types

Select...
4
3
3

Relationship

0
10

Authors

Journals

citations
Cited by 137 publications
(189 citation statements)
references
References 93 publications
11
177
1
Order By: Relevance
“…In summary, we have presented evidence that large distances separate coordinately expressed reporter genes regulated by a shared enhancer. These distances are consistent with the hub model, whereby clusters or condensates of Pol II or associated factors are recruited to active foci (2)(3)(4)21). Further support for this view is the correlation between the distances of separation and the size of transcriptional bursts (summarized in Fig.…”
Section: Significancesupporting
confidence: 82%
“…In summary, we have presented evidence that large distances separate coordinately expressed reporter genes regulated by a shared enhancer. These distances are consistent with the hub model, whereby clusters or condensates of Pol II or associated factors are recruited to active foci (2)(3)(4)21). Further support for this view is the correlation between the distances of separation and the size of transcriptional bursts (summarized in Fig.…”
Section: Significancesupporting
confidence: 82%
“…Furthermore, advances in live cell imaging will complement the continually improving structural and mechanistic models of Pol II transcription. Despite recent progress (Liu and Tjian 2018), many basic questions remain unanswered, such as (1) how genomes are organized in the three-dimensional space of the nucleus (Furlong and Levine 2018), (2) how transcriptional bursting occurs (Donovan et al 2019;Rodriguez et al 2019;Stavreva et al 2019), (3) how enhancer-promoter interactions are controlled (Lim et al 2018;Li et al 2019), (4) how enhancers actually work to activate gene expression (Benabdallah et al 2019;Heist et al 2019), (5) how gene expression patterns are maintained (i.e., active vs. repressed) through mitosis (Teves et al 2018), and so on. Also, what set of cofactors are essential for these processes, and which are redundant, context-specific, or cell type-specific?…”
Section: Concluding Remarks and Future Directionsmentioning
confidence: 99%
“…Transcriptional bursting of eukaryotic genes is the universal principle of gene expression with burst frequency ranging from minutes to hours and burst size from one to hundreds of transcripts (Chubb et al, 2006;Li et al, 2019;Nicolas et al, 2017). Given an RNAPII elongation speed of 3.8 kb/min (Singh and Padgett, 2009), short genes (<20 kb) are expressed within seconds to minutes and, therefore, at any given moment, a cell with a highly expressed short gene can have either both alleles active or pausing, or only one allele active.…”
Section: Highly Expressed Genes Are Expressed Biallelically and Exhibmentioning
confidence: 99%