Single Multiplex Polymerase Chain Reaction To Detect Diverse Loci Associated with Diarrheagenic<i>Escherichia coli</i>

López-Saucedo, Catalina; Cerna, Jorge F.; Villegas-Sepulveda, Nicolás; Thompson, R. A.; Velázquez, F. Raúl; Torres, Javier; Tarr, Phillip I.; Estrada-García, Teresa

doi:10.3201/eid0901.010507

Cited by 164 publications

(109 citation statements)

References 23 publications

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“…15,16 The polymerase chain reaction (PCR) for detecting virulence genes is commonly used, and PCR modifications such as multiplex PCR make them suitable as a routine test in developing countries, where expensive analyses are difficult to perform. 17,18 Phylogenetic studies show that E. coli strains associated with disease in humans has diversified and distributed into four distinct clonal groups. The commensal E. coli strains tend to associate within phylogenetic groups A and B1 and the extra-intestinal pathotypes within phylogenetic groups B2 and D. [19][20][21] We used a multiplex PCR based on the one described by López-Saucedo et al for detecting EPEC, ETEC, EHEC, and EIEC.…”

Section: Introductionmentioning

confidence: 99%

Diarrheagenic Escherichia coli in Children from Costa Rica

Pérez¹,

Gómez‐Duarte²,

Arias³

2010

The American Society of Tropical Medicine and Hygiene

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Abstract. More than 5,000 diarrheal cases per year receive medical care at the National Children's Hospital of Costa Rica, and nearly 5% of them require hospitalization. A total of 173 Escherichia coli strains isolated from children with diarrhea were characterized at the molecular, serologic, and phenotypic level. Multiplex and duplex polymerase chain reactions were used to detect the six categories of diarrheagenic E. coli . Thirty percent (n = 52) of the strains were positive, indicating a high prevalence among the pediatric population. Enteropathogenic E. coli and enteroinvasive E. coli pathotypes were the most prevalent (21% and 19%, respectively). Pathogenic strains were distributed among the four E. coli phylogenetic groups A, B1, B2, and D, with groups A and B1 the most commonly found. This study used molecular typing to evaluate the prevalence of diarrheagenic E. coli reported in Costa Rica and demonstrated the importance of these pathotypes in the pediatric population.

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Section: Introductionmentioning

confidence: 99%

Diarrheagenic Escherichia coli in Children from Costa Rica

Pérez¹,

Gómez‐Duarte²,

Arias³

2010

The American Society of Tropical Medicine and Hygiene

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“…Moreover, the analysis of molecular biomarkers has been used for some specific medical diagnoses. For examples, fecal protein serves as a sample for enzyme-linked immunosorbent assays (ELISA) (11), and fecal DNA has been examined for the identification of intestinal bacteria (12) and viruses causing gastroenteritis (13). For the purpose of analyzing gene expression, fecal total RNA must first be purified from frozen feces (14).…”

Section: Introductionmentioning

confidence: 99%

A homologue of the Drosophila headcase protein is a novel tumor marker for early-stage colorectal cancer

Chien

Chang²,

Yang

et al. 2006

Oncol Rep

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Abstract. The altered expression of certain genes is frequently detected as a hallmark of malignant tumors. These differentially expressed genes have the potential to become molecular markers. We purified the fecal mRNA from patients with colorectal cancer to identify novel candidates by using oligonucleotide microarray hybridization. We identified 21 upregulated and 22 downregulated candidates with significantly altered expression in patient fecal samples that have not been previously characterized. A gene encoding a homologue of the Drosophila headcase protein (HECA) was further examined due to its high ranking and possible importance in some human cancers. A tendency towards increased expression of HECA was dependent upon the clinicopathological stage. In this report, healthy individuals expressed less HECA, either in their blood samples or feces. Moreover, we detected upregulated HECA in blood and fecal samples of patients with colorectal cancer, and its expression level was shown to be significantly correlated with disease status. Our data show that HECA may be an early-stage classifier of colorectal cancer that can discriminate between late-and early-stage disease. In conclusion, this study is the first to analyze differentially expressed genes in the feces of colorectal cancer patients using oligonucleotide microarrays. The data suggest that HECA expression levels in feces may be an effective classifier for early-stage colorectal cancer.

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“…The aap gene is under the control of aggR. Earlier finding showed that EAEC isolates carrying aggR and aap may be phylogenetically or pathogenetically linked [3,11]. Dispersin is a secreted lowmolecular weight protein (10.2 kDa) that coats the bacterial surface and promotes dispersal of EAEC on the intestinal mucosa.…”

Section: Discussionmentioning

confidence: 99%