Single-Nanoparticle Collision Electrochemistry Biosensor Based on an Electrocatalytic Strategy for Highly Sensitive and Specific Detection of H7N9 Avian Influenza Virus

Abstract

Single-nanoparticle collision electrochemistry (SNCE) has gradually become an attractive analytical method due to its advantages in analytical detection, such as a fast response, low cost, low sample consumption, and in situ real-time detection of analytes. However, the biological analyte's direct detection based on the SNCE blocking mode has the problems of low sensitivity and specificity. In this work, an SNCE biosensor based on SNCE electrocatalytic strategy was used for the detection of H7N9 AIV. Nucleic acid aptamers were introduced to recognize the target virus (H7N9 AIV). After the recognition event, ssDNA 1 was released and hybridized with another ssDNA 2 . Owing to the nicking endonuclease Nt.AlwI-mediated target nucleic acid cyclic amplification, one virus particle can indirectly induce the release of 4.2 × 10 6 Au NPs that can be counted by the SNCE electrocatalytic strategy. The high conversion efficiency greatly improved the detection sensitivity, and the detection limit was as low as 24.3 fg/mL. Therefore, the constructed biosensor can achieve a highly sensitive and specific detection of H7N9 AIV and show a great potential in bioanalytical application.

Aluminum(III)-Based Organic Nanofibrous Gels as an Aggregation-Induced Electrochemiluminescence Emitter Combined with a Rigid Triplex DNA Walker as a Signal Magnifier for Ultrasensitive DNA Assay

“…Notably, the performance comparison of the constructed sensing platform with other methods confirmed that the strategy possessed a wide linear range and low LOD, exhibiting its ultrasensitive analytical performance (Table S3). − Meanwhile, the target fDNA in dilute fresh human serum samples was quantified according to the above linear equation, and the corresponding target recovery was calculated to be 96–102%, indicating the preliminary reliability and practicability of this strategy in real samples (Table S4).…”

Artificial Intelligence-Assisted Multiparameter Size Discrimination of Silver Nanoparticles through Electrochemical Collision

“…Nanoparticles (NPs) have a wide range of applications in different fields, including electrocatalysis, electrochemical sensing, antimicrobial and virucidal research, due to their unique physical and chemical properties. − The properties of NPs are strongly related with their size and shape. − Single nanoparticle collision, as a prominent electrochemical detection method, can provide information about the surface charge, size distribution, aggregation, and other properties of single nanoparticle based on analysis of current signals generated by the collision between the nanoparticle and the electrode, − where the nanoparticle may adsorb and block the flux of redox-active molecules and autoelectrochemically react or enable a catalytic reaction . It is worth noting that this method has been used in the analysis of metal nanoparticles and quantification of analytes in the bioanalytical field at the single-particle level. − …”

Rapid Direct Detection of SARS-CoV-2 Aerosols in Exhaled Breath at the Point of Care

“…This methodology has limitations for mass testing applications due to long turnaround times and the need for sophisticated equipment and trained personnel. Electrochemical biosensors have previously been used for the detection of influenza − viruses (see Supporting Materials (SM) Table S2) and are rapidly emerging as an alternative to conventional clinical screening techniques. These sensing systems are simple, accurate, and possess a low limit of detection.…”