Single-nuclei multiomic analyses identify human cardiac lymphatic endothelial cells associated with coronary arteries in the epicardium

Travisano, Stanislao Igor; Harrison, Michael R.M.; Thornton, Matthew E.; Grubbs, Brendan H.; Quertermous, Thomas; Lien, Ching-Ling

doi:10.1016/j.celrep.2023.113106

Cited by 7 publications

(3 citation statements)

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“…To profile the development of the lymphatic vessels alongside the coronary artery vasculature, a recent study performed multiomic profiling (snRNA/ATAC-seq) of subepicardial endothelial cells in fetal hearts at 10 to 11 postconception weeks. 173 This study proposed an interaction between VEGF + arterial endothelial cells and lymphatic endothelial cells that shows a gene ontology analysis term enrichment for VEGF signaling; arterial endothelial cells may guide lymphatic endothelial cell differentiation to develop the lymphatics surrounding the coronary arteries. 173 Related to the immune function of lymphatic vessels, CCL21 + lymphatic endothelial cells have been detected by a single-cell study of endothelial cells in ventricular sections.…”

Section: Context: Cardiac Cells and Their Microenvironmentsmentioning

confidence: 95%

“… 173 This study proposed an interaction between VEGF + arterial endothelial cells and lymphatic endothelial cells that shows a gene ontology analysis term enrichment for VEGF signaling; arterial endothelial cells may guide lymphatic endothelial cell differentiation to develop the lymphatics surrounding the coronary arteries. 173 Related to the immune function of lymphatic vessels, CCL21 + lymphatic endothelial cells have been detected by a single-cell study of endothelial cells in ventricular sections. 174 Staining of human epicardial sections has indicated CCL21 expression in the left ventricle epicardial region.…”

Section: Context: Cardiac Cells and Their Microenvironmentsmentioning

confidence: 95%

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Revisiting Cardiac Biology in the Era of Single Cell and Spatial Omics

Palmer,

Rosenthal,

Teichmann

et al. 2024

Circulation Research

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Throughout our lifetime, each beat of the heart requires the coordinated action of multiple cardiac cell types. Understanding cardiac cell biology, its intricate microenvironments, and the mechanisms that govern their function in health and disease are crucial to designing novel therapeutical and behavioral interventions. Recent advances in single-cell and spatial omics technologies have significantly propelled this understanding, offering novel insights into the cellular diversity and function and the complex interactions of cardiac tissue. This review provides a comprehensive overview of the cellular landscape of the heart, bridging the gap between suspension-based and emerging in situ approaches, focusing on the experimental and computational challenges, comparative analyses of mouse and human cardiac systems, and the rising contextualization of cardiac cells within their niches. As we explore the heart at this unprecedented resolution, integrating insights from both mouse and human studies will pave the way for novel diagnostic tools and therapeutic interventions, ultimately improving outcomes for patients with cardiovascular diseases.

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Section: Context: Cardiac Cells and Their Microenvironmentsmentioning

confidence: 95%

Section: Context: Cardiac Cells and Their Microenvironmentsmentioning

confidence: 95%

Revisiting Cardiac Biology in the Era of Single Cell and Spatial Omics

Palmer,

Rosenthal,

Teichmann

et al. 2024

Circulation Research

View full text Add to dashboard Cite

show abstract

“…3,4 Furthermore, recent clinical studies have demonstrated that cardiac lymphangiogenesis occurs in patients experiencing primary or secondary hypertrophic or dilated cardiomyopathy. 5,6 Lymphatic vessels, located in the epicardium of human fetal hearts, are associated mainly with coronary arteries that produce VEGF-C (vascular endothelial growth factor C), 7 essential for coronary lymphangiogenesis during development. In embryos, after arteriovenous endothelial differentiation controlled by the NOTCH (neurogenic locus notch homolog protein) signal transduction pathway, the NR2F2/COUP-TFII (COUP transcription factor), SOX18 (SRY-related HMG-box), and PROX1 (prospero homeobox protein-1) transcription factors trigger the lymphatic differentiation program in venous vessels.…”

Section: See Cover Imagementioning

confidence: 99%