Single Nucleotide Polymorphisms in Gh, Ghr, Ghsr and Insulin Candidate Genes in Chicken Breeds of Vietnam

Khoa, Do Vo Anh; Khang, Nguyen Thi Kim; Ngu, Nguyen Trong; Joseph, Matey,; Loan, Huynh Thi Phuong; Thúy, Nguyễn Thị Diệu

doi:10.15580/gjas.2013.3.042613589

Cited by 9 publications

(12 citation statements)

References 15 publications

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“…Polymorphism study in the g.565G>A GHR locus found that the AA genotype had the highest frequency (0.726) than the AG (0.172) and GG (0.102) in overall population. Similar result was reported in Vietnamese (0.86, Khoa et al, 2013), Noi (1.00, Khoa et al, 2013), Xinghua (1.00, Lei et al, 2007), and White Plymouth Rock (1.00, Lei et al, 2007) chickens. Ouyang et al (2008) could not detect the g.565G>A GHR polymorphism using DHPLC detection in Leghorn layer, White Recessive Rock broiler, Taihe Silkies, and Xinghua chickens.…”

Section: Discussionsupporting

confidence: 88%

The Associations of GH and GHR Genes with Carcass Components in Indonesian Kampung and Broiler Chicken Cross

Khaerunnisa

Jakaria

Arief

et al. 2017

Med Pet

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The chicken growth hormone (GH) and its receptor (growth hormone receptor, GHR) play important roles in chicken performances due to their crucial functions in growth. The variations of GH and GHR genes were then thought to be associated with the variations of the performances. This experiment was designed to identify the g.2248G>A GH and the g.565G>A GHR loci polymorphisms and to evaluate their associations with carcass components in Kampung and broiler chicken cross. A total of 215 chickens including 4 chicken populations (Kampung, Cobb broiler, F1, and F2 Kampung x broiler chicken cross) were screened to identify polymorphism using PCR-RFLP technique with EcoRV and Eco72I restriction enzyme for GH and GHR loci, respectively. The carcass components were recorded at 26 weeks of age on F2 Kampung and broiler chicken cross (42 chickens) for association study. Both the g.2248G>A GH and the g.565G>A GHR loci were polymorphic with two alleles (G and A) and three genotypes (GG, AG, and AA). The GG genotype and the G allele of GH locus were predominant in all chicken populations. While in GHR locus, the AA genotype and the A allele were found to be higher in all chicken populations. The association study showed that the g.565G>A GHR locus polymorphism had significant effect on carcass components, including live weight, carcass weight, breast weight, thighs weight, breast muscle weight, and thighs muscle weight. There was no significant association was found between the g.2248G>A GH genotype and carcass components. It could be concluded that the g.2248G>A GH and the g.565G>A GHR loci were polymorphic in Kampung and broiler chicken cross and the g.565G>A GHR locus was accosiated with carcass components. This g.565G>A GHR SNP might be an important candidate marker for chicken growth and muscle mass improvement. Keywords: Kampung chicken cross, growth hormone, growth hormone receptor, gene polymorphisms, carcass ABSTRAKHormon pertumbuhan dan reseptor hormon pertumbuhan memiliki peran penting dalam performa pertumbuhan ayam. Tujuan penelitian ini adalah mengidentifikasi keragaman gen hormon pertumbuhan (lokus g.2248G>A GH) dan gen reseptor hormon pertumbuhan (lokus g.565G>A GHR) dan mengevaluasi pengaruhnya pada komponen karkas pada ayam silangan Kampung dan broiler. Penentuan genotipe dilakukan pada 215 ekor ayam, yang terdiri atas 4 populasi (Kampung, ras pedaging strain Cobb, silangan F1 dan F2 Kampung x ras pedaging strain Cobb) menggunakan enzim pemotong EcoRV dan Eco72I masing-masing untuk fragmen lokus g.2248G>A GH dan lokus g.565G>A GHR. Analisis asosiasi dengan komponen karkas dilakukan pada populasi ayam silangan F2 Kampung x ras pedaging strain Cobb umur 26 minggu. Kedua lokus tersebut bersifat polimorfik dengan dua alel yang ditemukan (alel A dan G) dan tiga genotipe (genotipe GG, AG, dan AA). Genotipe GG dan alel G pada lokus g.2248G>A GH ditemukan dalam frekuensi tertinggi di semua populasi yang diuji. Sementara pada lokus g.565G>A GHR, genotipe AA dan alel A ditemukan paling dominan. Analisis asosiasi...

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Section: Discussionsupporting

confidence: 88%

The Associations of GH and GHR Genes with Carcass Components in Indonesian Kampung and Broiler Chicken Cross

Khaerunnisa

Jakaria

Arief

et al. 2017

Med Pet

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“…At G662A of GH gene, the AA genotype frequency in Mia breed was higher (0.50) as compared to the GG frequency (0.03). This result is consistent with a previous study, which showed that the AA genotype was major and GG was minor in local chicken breeds in Vietnam (Khoa et al, 2013). At the C423T SNP of GH gene, CC genotype had a higher frequency than TT.…”

Section: Results and Discussion Polymorphisms Of Ins And Gh Genessupporting

confidence: 93%

“…For the T3737C SNP of INS, no CC genotype was observed. In previous studies, the CC genotype was observed with quite low frequency: 1% in the Coob lines and 3% in the Noi chicken breed ( Khoa et al, 2013). The reason of non-occurrence of the CC genotype in this study is probably due to the low frequency of the C allele in this population and the small sample size (expected number of CC under HWE conditions is only 1.72).…”

Section: Results and Discussion Polymorphisms Of Ins And Gh Genescontrasting

confidence: 61%

Association of single nucleotide polymorphisms in the insulin and growth hormone gene with growth traits of Mia Chicken

Thinh

Tuấn

Vinh

et al. 2019

IJAR

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This study was conducted in Mia chicken breed to evaluate the association between four single nucleotide polymorphisms (SNPs), in insulin (INS) and growth hormone (GH) genes, with growth traits. Three genotypes for the SNP A3971G of INS gene and the SNP G662A and C423T of GH gene were present in the population while only two genotypes were found in the Mia chicken breed for SNP T3737C of INS gene (TT and TC). The SNP T3737C INS gene and G662A GH gene had significant association with growth traits (P < 0.05). A significant association of T3737C INS gene with body weight (BW) was observed at 10 to 12 weeks of age and average daily gain (ADG) at 6-8 weeks of age. The SNP G662A of the GH gene was significantly associated (P < 0.05) with BW of Mia chicken at ages from 7 to 14 weeks and with ADG (4-6; 6-8; 8-10; 10-12 and 2-16 weeks). Chicken with the GG genotype had greater BW and ADG compared to the other genotypes. The results demonstrated that this SNP G662A GH gene may be used as a candidate marker gene for genetic improvement of growth traits in Mia chicken breed.

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“…En 12 12 and En 40 are the number of eggs for 12 and 40 weeks of productive period, respectively; Ew 30 and Ew 52 are the egg weight on the week 30 and the week 52 weeks of life, respectively; MspI, SacI are restriction endonucleases. Refer to the body of the article for genotype description by allelic variants.…”

Section: Productivity Character Genotypementioning

confidence: 99%

“…It was shown that several different types of SNP's (G565A, A1512T, etc.) affecting the intronic and exonic parts, as well as nontranslated 5 and 3 gene regions, are present [8,12,13]. It was found that the allelic variants of the GHR gene are associated with quality indices of shell and live weight, as well as with chicken dwarfism.…”

mentioning

confidence: 99%

Polymorphism of Growth Hormone, Growth Hormone Receptor, Prolactin and Prolactin Receptor Genes in Connection With Egg Production in Poltava Clay Chicken

Kulibaba¹

2015

S-h. biol.

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A b s t r a c tThe molecular assisted selection in animals is based on polymorphism study of desired gene alleles related to economic important traits, particularly productivity parameters. Allelic variants can be a result of different modification of DNA nucleotide composition, i.e. spot mutations leading to a single nucleotide polymorphism (SNP), insertions and deletions (indel), etc. Anyway, targeted selection necessitates detecting gene polymorphism and estimating its relationship with productivity. The genes encoding proteins which are involved in the growth and development regulation, such as hormones, are considered the most perspective and attractive candidates. So far as the physiological effect of any hormone is known to be directly related to its receptor, it is advisable to study gene polymorphism of both hormones and their receptors. In the Ukraine the relationship between polymorphism of the genes mentioned hereinabove and hen performance was not still studied. Therefore these stipulate the relevance and novelty of our investigation presented in the paper. The genetic structure of Poltava Clay chicken egg-and-meat breed (line 14, n = 98, laboratory population of State Poultry Research Station of the National Academy of Agrarian Sciences of Ukraine) was studied on growth hormone (GH), growth hormone receptor (GHR), prolactin (PRL) and prolactin receptor genes (PRLR). Gene polymorphism was determined by PCR-RFLP analysis and by comparative analysis of amplified fragment length when studying insertion in the prolactin locus. The growth hormone, growth hormone receptor and prolactin genes were polymorphic. The frequencies obtained were as follows: 0.372 and 0.628 for alleles C and T (PRL-AluI), respectively, in GH-MspI (intron 1) 0.908 for alleles A, 0.020 for B, 0.072 for C; in GH-SacI (the intron 4) 0.036 for A, 0.964 for B; in GHR-NspI 0.280 for A, 0.720 for B. The prolactin gene (24 indel) and prolactin receptor gene were monomorphic. The relationship of different genotypes of prolactin, growth hormone, and growth hormone receptor genes with egg productivity was studied. All studied polymorphic loci were in Hardy-Weinberg equilibrium in population of Poltava clay chicken breed. It is shown the significant differences in the productivity of individuals of different genotypes for the loci of prolactin (PRL-AluI) and growth hormone (GH-SacI). The egg production for 12 weeks in the individuals with genotype CC-PRL exceeded that in the individuals with genotype TT-PRL and amounted to 75.38±2.33 and 67.82±1.21, respectively (P < 0.01). Egg production for 40 weeks in the chickens with genotype CC-PRL reached 201.50±8.43, and in TT-PRL hens it was 188.32±3.45. The egg weight at 30 th week of life was 54.80±1.44 g in the individuals with CC-PRL genotype against 50.95±0.54 g in the individuals with CT-PRL genotype (P < 0.05). At the same time the heterozygous individuals with genotype AB (SacI +/SacI) on growth hormone gene were characterized by a significantly higher egg production during 12 week productive...

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Single Nucleotide Polymorphisms in Gh, Ghr, Ghsr and Insulin Candidate Genes in Chicken Breeds of Vietnam

Cited by 9 publications

References 15 publications

The Associations of GH and GHR Genes with Carcass Components in Indonesian Kampung and Broiler Chicken Cross

The Associations of GH and GHR Genes with Carcass Components in Indonesian Kampung and Broiler Chicken Cross

Association of single nucleotide polymorphisms in the insulin and growth hormone gene with growth traits of Mia Chicken

Polymorphism of Growth Hormone, Growth Hormone Receptor, Prolactin and Prolactin Receptor Genes in Connection With Egg Production in Poltava Clay Chicken

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