Single nucleotide polymorphisms in Heat Shock Protein (HSP) 90AA1 gene and their association with heat tolerance traits in Sahiwal cows

Kumar, Rakesh; Gupta, I. D.; Verma, Nishant; Vineeth, M R

doi:10.18805/ijar.7081

Cited by 8 publications

(12 citation statements)

References 21 publications

(19 reference statements)

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“…The respiration rate is a reliable physiological parameter for predicting heat stress in dairy cattle (Dalcin et al, 2016) and increases with THI. Published literature also revealed higher respiration rates in cattle during summer, when THI values increase (Kumar et al, 2015;Sailo et al, 2015a;Verma et al, 2015 andDas, 2014). The increase in respiration rate in summer as compared to other seasons may be due to the more demand for oxygen by the tissues in stressful conditions.…”

Section: Respiration Rate (Rr)mentioning

confidence: 92%

“…58 ± 0.38, 25.94 ± 0.38 and 21.90 ± 0.38 in summer, rainy and winter seasons respectively. Several authors (Kumar et al, 2015;Sailo et al, 2015a;Verma et al, 2015 andDas, 2014) also reported higher respiration rates of Sahiwal cattle and (Deb et al, 2013;Sailo et al, 2015b;Verma et al, 2015 andDas, 2014) in HF crossbred cattle during summer as compared to winter season. Increased respiration is an important physiological response, which aids in dissipation of excess body heat by vaporizing more moisture in the expired air (Atkins et al, 2018).…”

Section: Respiration Rate (Rr)mentioning

confidence: 95%

“…Higher values of heat tolerance coefficients were observed in summer when THI was above 83, as compared to the other two seasons in the present study. Kumar et al (2015), Sailo et al (2015a), Das, (2014…”

Section: Heat Tolerance Coefficientmentioning

confidence: 99%

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Effect of Seasons on Physiological Responses in Sahiwal and Crossbred Cows

Prasanna

Rao

Prakash

et al. 2021

IJAR

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Background: Growing demand for improving milk production and rising temperatures due to global warming has increased the thermal load on dairy animals. Physiological parameters such as respiration rate and body temperature mainly determine the adaptability of animals to climate stress. During genetic adaptation, Bos indicus cattle have acquired thermo tolerant genes and when exposed to heat stress conditions, the Bos indicus cattle have lower respiration rates and rectal temperatures than Bos taurus animals. The present study was aimed to study the effect of seasons on the physiological responses in Sahiwal and crossbred cows. Methods: A total of 50 crossbred cows maintained at Military dairy Farm, Secunderabad and 50 Sahiwal cows maintained at Livestock Farm Complex, College of Veterinary Science, Rajendranagar, PVNRTVU were utilized for the present investigation. The study was conducted during summer (THI = 83.71±0.01), rainy (THI = 71.37±0.01) and winter (THI = 66.69±0.01) seasons. Changes in respiration rate (RR), rectal temperature (RT) and heat tolerance coefficient (HTC) were observed in different seasons in Sahiwal and crossbred cows. Result: RR (breaths/ min) in Sahiwal cows during summer, rainy and winter were 28.56±0.38, 23.38±0.38 and 20.54±0.38 respectively whereas RR (breaths/ min) in crossbred cows were 44.58±0.38, 25.94±0.38 and 21.90±0.38 respectively. In Sahiwal cows RT (°C) during summer, rainy and winter were 38.52±0.03, 38.23±0.03 and 38.13±0.03 respectively whereas RT (°C) in crossbred cows were 39.22±0.02, 38.72±0.03 and 37.80±0.03 respectively. The magnitude of increase in RR, RT and HTC were found to be higher during summer compared to other seasons in both Sahiwal and crossbred cows. It was observed that Sahiwal cows are less sensitive to heat stress and are better able to regulate their body temperature than crossbred cows when environmental temperature increases during summer. The HTC values were lower in Sahiwal cows in all the seasons studied indicating better thermo tolerance when compared to the crossbred cows.

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Section: Respiration Rate (Rr)mentioning

confidence: 92%

Section: Respiration Rate (Rr)mentioning

confidence: 95%

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Effect of Seasons on Physiological Responses in Sahiwal and Crossbred Cows

Prasanna

Rao

Prakash

et al. 2021

IJAR

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“…HSP90AA1 belongs to the group of heat shock proteins (HSPs) which play a vital role in protecting cells from environmental stress. Kumar et al ( 113 ) conducted a study in Sahiwal cows to identify SNPs in exons 3, 7, 8, and 11 of HSP90AA1 and study its association with thermal tolerance. They reported a total of five SNPs (A1209G, A3292C, T4935C, T5218C, and A5224C) in this breed of indigenous cattle, among which two, A1209G and A3292C, had a significant association with heat tolerance traits.…”

Section: Biomarkers For Identifying Thermo-tolerant Dairy Cowmentioning

confidence: 99%

Climate-Resilient Dairy Cattle Production: Applications of Genomic Tools and Statistical Models

et al. 2021

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The current changing climate trend poses a threat to the productive efficacy and welfare of livestock across the globe. This review is an attempt to synthesize information pertaining to the applications of various genomic tools and statistical models that are available to identify climate-resilient dairy cows. The different functional and economical traits which govern milk production play a significant role in determining the cost of milk production. Thus, identification of these traits may revolutionize the breeding programs to develop climate-resilient dairy cattle. Moreover, the genotype–environment interaction also influences the performance of dairy cattle especially during a challenging situation. The recent advancement in molecular biology has led to the development of a few biotechnological tools and statistical models like next-generation sequencing (NGS), microarray technology, whole transcriptome analysis, and genome-wide association studies (GWAS) which can be used to quantify the molecular mechanisms which govern the climate resilience capacity of dairy cows. Among these, the most preferred option for researchers around the globe was GWAS as this approach jointly takes into account all the genotype, phenotype, and pedigree information of farm animals. Furthermore, selection signatures can also help to demarcate functionally important regions in the genome which can be used to detect potential loci and candidate genes that have undergone positive selection in complex milk production traits of dairy cattle. These identified biomarkers can be incorporated in the existing breeding policies using genomic selection to develop climate-resilient dairy cattle.

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“…The most common and temperature-sensitive is HSP90, which is thought to play an important role in environmental stress and thermal adaptation (Gade et al, 2010;Aritonang et al, 2017;Puteri et al, 2018). In summer, higher levels of expression of proteins of the HSP70 and HSP90 families have been observed in a variety of livestock species (Archana et al, tolerance, milk production, fertility, and disease susceptibility in livestock (Shergojry et al, 2014a;Kumar et al, 2015;Bhat et al, 2016). They may be useful as candidate gene markers for identifying animals with enhanced climatic resistance, immunological response, and performance (Hassan et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Identification of Polymorphism within Exon 8 of Bovine HSP90AA1 Gene using PCR-SSCP Technique

Prasanna¹,

Rao²,

Prakash³

et al. 2021

IJBSM

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Heat shock proteins (HSPs) are a type of molecular chaperones that aid in the recovery of stressed cells and serve as a major system for intracellular self-defense. A study was conducted during the year 2018–19 at College of Veterinary Science Rajendranagar, Hyderabad, Telengana State, India to find polymorphisms in exon 8 of the bovine HSP90AA1 gene in Sahiwal (n=50) and crossbred (n=50) cows. Blood samples were collected from the experimental animals and genomic DNA was isolated. Physiological parameters like body temperature and respiration rate for each animal were taken during the experimental period and the heat tolerance coefficient was calculated. The data on production and reproduction traits were obtained from the history sheets of the animals. To detect the polymorphism, a 539 bp fragment of the HSP90AA1 gene covering exon 8 was subjected to the Polymerase Chain Reaction-Single-Strand Conformation Polymorphism (PCR-SSCP) technique.The PCR-SSCP of exon 8 of HSP90AA1 gene yielded two genotypic patterns AA and AB corresponding to two allelic variants with frequencies of 0.85, 0.15, 0.81 and 0.19 in Sahiwal and crossbred cows, respectively. The PCR-SSCP patterns obtained were correlated with the physiological, productive, and reproductive traits in both Sahiwal and crossbred cows. The association analysis of SSCP patterns of the exon 8 of HSP90AA1 gene revealed non-significant effect in Sahiwal cows, although the AB genotype had a significantly longer service period in crossbred cows.

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Single nucleotide polymorphisms in Heat Shock Protein (HSP) 90AA1 gene and their association with heat tolerance traits in Sahiwal cows

Cited by 8 publications

References 21 publications

Effect of Seasons on Physiological Responses in Sahiwal and Crossbred Cows

Effect of Seasons on Physiological Responses in Sahiwal and Crossbred Cows

Climate-Resilient Dairy Cattle Production: Applications of Genomic Tools and Statistical Models

Identification of Polymorphism within Exon 8 of Bovine HSP90AA1 Gene using PCR-SSCP Technique

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