Single Nucleotide Polymorphisms in the 3' Untranslated Region of Vascular Endothelial Growth Factor Gene in Japanese Population with or without Renal Cell Carcinoma.

Abstract

We investigated native Japanese subjects whether C702T, C936T and G1612A polymorphisms in the 3' untranslated region (3'-UTR) of vascular endothelial growth factor (VEGF) gene are associated with the risk of renal cell carcinoma (RCC). Genomic DNAs from 145 RCC patients and 145 healthy controls were examined by polymerase chain reaction-based restriction fragment length polymorphism. Variant allele frequencies of C702T, C936T and G1612A were 0.00, 0.20 and 0.13 in the controls, respectively. The C702T and G1612A allele frequencies were significantly different between the Japanese population and the Caucasian population reported elsewhere. For each of C936T and G1612A polymorphisms, there was no statistically significant difference in the distribution of genotype frequencies between the cases and controls. Odds ratios and 95% confidence intervals computed by logistic regression analyses were not statistically significant. Stratification for the RCC cases according to pathological cell subtype, grade or stage failed to reveal any significant heterogeneity with respect to the genotype of each VEGF polymorphism. We revealed that there are significant ethnic differences in the C702T and G1612A allele frequencies, but suggested that C702T, C936T and G1612A polymorphisms in the 3'-UTR of VEGF gene are not associated with the risk of RCC, at least in Japanese population.

Influence of VEGF‐A gene variation and protein levels in breast cancer susceptibility and severity

“…10,11,[23][24][25] The role of these polymorphisms in diseases including solid cancers has been the subject of several recent publications. In the Ônon breast cancerÕ literature, the 21154G > A and the 2460C > T polymorphisms have been associated with prostate cancer risk, 26,27 while no significant associations have been found in patients with malignant melanoma, 28 renal cell cancer 29 and high grade gliomas. 30 In breast cancer, the 936C > T polymorphism was first reported in a study of 500 breast cancer patients and 500 matched controls, with reduced breast cancer risk in carriers of the T allele [OR 5 0.51; 95%CI 5 0.38-0.70].…”

Vascular endothelial growth factor +936C/T polymorphism and cancer risk in Asians: a meta-analysis

“…In total, 21 studies (Abe et al, 2002;Kataoka et al, 2006;Hsiao et al, 2007;Amano et al, 2008;Bae et al, 2008a,b;Chae et al, 2006Chae et al, , 2008Cheng et al, 2008;Ke et al, 2008;Hsing et al, 2008;Al-Moundhri et al, 2009;Tahara et al, 2009;Wu et al, 2009;Li et al, 2010;Kim et al, 2010;Bao et al, 2011;Beeghly-Fadiel et al, 2011;Kang et al, 2011;Zhou et al, 2011) comprising 8298 cancer cases and 8053 controls were included in the meta-analysis. The VEGF allele +936T frequency was 17.78% in cases and 17.84% in controls.…”

VEGF 936C>T is predictive of threshold retinopathy of prematurity in Japanese infants with a 30-week gestational age or less

“…Using the public National Center for Biotechnology Information Single Nucleotide Polymorphism database and available literature, we selected six VEGF candidate polymorphisms, ie, −1498T.C (rs833061) and −1154G.A (rs1570360) in the promoter region, −634C.G (rs2010963) and −7C.T (rs25648) in the 5' UTR, and 936C.T (rs3025039) and 1612G.A (rs10434) in the 3' UTR. Each polymorphism showed a minor allele frequency of at least 0.10 in the Japanese population, 23,[32][33][34][35] and all polymorphisms were investigated in terms of their association with various types of diseases or clinical situations. 22,[36][37][38][39] The six VEGF genotypes were evaluated using TaqMan R minor groove binding probe-based polymerase chain reaction, and confirmed by direct sequencing, as described previously.…”