Single Nucleotide Polymorphisms linked to the SlMYB12 Gene that Controls Fruit Peel Color in Domesticated Tomatoes (Solanum lycopersicum L.)

Kim, Bichsaem; Kim, Nahui; Kang, Jum-Soon; Choi, Young-Kiu; Sim, Sung-Chur; Min, Sung Ran; Park, Young-Hoon

doi:10.7235/hort.2015.15032

Cited by 6 publications

(4 citation statements)

References 19 publications

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“…With the rapid development of high-throughput sequencing technology and reduction in sequencing costs, the use of resequencing data to fine map genes and develop InDel markers closely linked to genes has been extended to several crops, including cucumbers [ 26 ], tomatoes [ 27 ], Brassica napus [ 28 ], melons [ 29 ], and watermelons [ 30 ]. Genes that regulate fruit shape and peel colour have been identified in many crops, and gene-linked InDel markers related to fruit shape have been developed in watermelons [ 31 ], cucumbers [ 32 ], and melons [ 33 ]; those associated with peel colour have been developed in peppers [ 34 ], eggplants [ 35 ], tomatoes [ 36 ], cucumbers [ 37 ], chieh qua [ 3 ], melons [ 38 ], and watermelons [ 39 ]. Developing InDel markers linked to genes using InDel-marker-assisted selection breeding can determine phenotypes from genotypes, reduce costs, and improve process efficiency.…”

Section: Discussionmentioning

confidence: 99%

Development and Application of InDel Markers Linked to Fruit-Shape and Peel-Colour Genes in Wax Gourd

Huang

et al. 2022

Genes

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The wax gourd is commonly grown in many countries because of its high nutritional and economic value. While the genes for the fruit shape and peel colour of wax gourd have been reported, the InDel markers linked to these genes remain undeveloped. In this study, the InDel markers linked to fruit-shape (Bch02G016830) and peel-colour (Bch05G003950) genes were developed from resequenced data. We used 120 inbred lines, 536 isolated populations, and 4 commercial hybrids to evaluate the validity and application value of the InDel markers. The accuracy rates of nine pairs of fruit-shape InDel markers (GX1-GX9) were 84.16–91.66% in 120 inbred lines. The accuracy rates of 27 pairs of peel-colour InDel markers (PS1-PS27) within approximately 3.0 Mb upstream and 3.0 Mb downstream of the peel-colour gene were 100% and those of 6 pairs of peel-colour InDel markers (PS28-PS33) within 3.0–20 Mb upstream and downstream of the peel-colour gene were 55.83–90% in 120 inbred lines. The purity of four commercial hybrids determined using GX1, GX2, PS13, and PS14 was highly consistent with the field results for purity determination. Our results provide important information for genetic linkage map construction, molecular-marker-assisted selective breeding, and purity determination of wax gourd hybrids.

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Section: Discussionmentioning

confidence: 99%

Development and Application of InDel Markers Linked to Fruit-Shape and Peel-Colour Genes in Wax Gourd

Huang

et al. 2022

Genes

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“…аллелей генов High pigment (hp-1, hp-2 dg ), обеспечивающих высокое накопление пигментов в плодах [14,15]; -аллелей гена SlMYB12 (Y и y) регулирующего накопление флавоноида халкона в кожуре плодов томата [16,17].…”

Section: материалы и методы исследованийunclassified

“…ние особенностей накопления ликопина в плодах при сочетании вышеописанных аллелей с аллелями гена SlMYB12 (Y и y), регулирующего накопление флавоноида халкона в кожуре плодов томата. Сочетание аллелей og c и b с аллелем Y дает малиновую и красную окраску, а с аллелем y -розовую [16,17]. Используя разработанные ранее маркеры [10], в том числе гена SlMYB12, было проведено ДНК-типирование изучаемых аллелей и определение уровня накопления каротиноидов в образцах, в том числе шести розовоплодных гибридов селекции ССА «Ильинична», ВНИИО -филиал ФГБНУ ФНЦО.…”

Section: результаты и их обсуждениеunclassified

Development of high-lycopene tomato hybrids using conventional breeding techniques and molecular markers

2020

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Relevance. High lycopene fruit content has been regarded as a very important genetic trait in tomato breeding. Use lycopene molecular markers in combination with conventional breeding techniques allowed us to create hybrids with high lycopene accumulation, excellent organoleptic qualities, high yield production and resistance to pathogens, and to effectively optimize our breeding programmes for commercial greehouses production.Material and Methods. In this study tomato samples including selected lines and hybrids with various allelic combinations of genes determining carotene accumulation, and other genetic traits, such as disease resistance and yield production were tested. Introgression of spontaneous and induced mutations was used to increase carotenoid levels (og and hp) and improve fruit technological qualities (nor, alc, rin). The research material was tomato collection, mutants, breeding lines and hybrids listed in the State Register Russian Federation tomato hybrids of breeding SS Agrofirm "Ilyinichna" VNIIO branch of the All-Russian Scientific Research Institute of Vegetable Growing – Branch of the FSBSI Federal Scientific Vegetable Center. DNA typing of fruit quality genes was performed at the Institute of Genetics and Cytology of the National Academy of Sciences of Belarus.Results. New domestic hybrids for industrial greenhouses, which characterised by improved organoleptic qualities and technological traits were developed with the help of phasedcross-breeding that allowed to combine the genes nor, rin, alc, leading to an extension of the shelf life with the genes B, og, hp1, etc., contributing to an increase in carotenoid content in fruits. It was established that for targeted selection and hybridization, despite the negative influence of the nor, rin, alc genes it is possible to raise the level of carotenoids to average values. Correlation between lycopene concentration in fruits and high temperature and level of insolation was confirmed. It was shown that pink-fruited forms contain significantly more lycopenethanred-fruitedones. Different all eliccombinations of structural genes involved in carotenoids biosynthesis and regulatory genes that provided maximal accumulation of lycopene in hybrid swithred and pink fruits were revealed. Hybrids with the combination of high concentrations of sugar (° Brix), dry matter and maximal lycopene values, combined defining excellent taste were selected: Prekrasnaiya lady, Olya, Quadrille, Victoria. New F1 hybrids one for industrial greenhouses: G950, G956, G960, Magistral and pink fruited G12897, surpassed the Dutch standard in productivity up to 21%, and in tastes/organoleptic qualities for 1-1.8 points.

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“…При этом B. Kim и соавт. при изучении полиморфизма данного гена связывают нарушение синтеза халкона с присутствием семи однонуклеотидных замен в экзонах, не находя отличий в промоторной области гена [8].…”

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Analysis of SlMYB12 gene polymorphism determining chalcone-naringenin biosynthesis in the skin of tomato fruits and its effect on the lycopene accumulation.

et al. 2020

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Efficiency in detecting of tomato forms with no chalcone-naringenin flavonoid in pink-fruited and yellow-fruited forms was evaluated using DNA markers for various polymorphisms of the SlMYB12 gene. The closest relationship between a phenotype with the transparent skin of fruits and a deletion in the promoter region of the SlMYB12 gene was shown. The highest efficiency in the detection of the recessive y allele of the regulatory SlMYB12 gene, leading to the chalcone-naringenin synthesis disruption and skin transparency, was established by a combination of markers MYB12-603delaF1/603del-aR6 (Myb-603del aF1/R6) and MYB12-603del-aF1/603del-aR5 (Myb12 aF1/R5). Fruit coloration peculiarities were shown depending on a combination of the structural alleles of a carotenoid biosynthesis pathway and SlMYB12 gene alleles. A combination of this y allele with the alleles of the gene of the lycopene-β-cyclase beta (b) and old gold crimson (ogc ) allows selecting pink and raspberry forms respectively. In tomato accessions with yellow and orange fruits, the y allele provides pale shades of the main coloration determined by carotenoid biosynthesis genes (yellow flesh (r), tangerine (t), Beta (B)). The presence of SNP T → C of the SlMYB12 gene (171476848 position of chromosome 1) was identified in 80 % of accessions with the transparent skin of fruits of the evaluated collection. The effect of the recessive y allele of the SlMYB12 gene on an increase in the lycopene concentration of tomato fruits in a combination with b, ogc alleles was shown. Using MAC methods by fruit quality genes, including the SlMYB12 gene, the cherry tomato variety Malinovyj koktel with a high lycopene accumulation was developed and included in the State Register

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Single Nucleotide Polymorphisms linked to the SlMYB12 Gene that Controls Fruit Peel Color in Domesticated Tomatoes (Solanum lycopersicum L.)

Cited by 6 publications

References 19 publications

Development and Application of InDel Markers Linked to Fruit-Shape and Peel-Colour Genes in Wax Gourd

Development and Application of InDel Markers Linked to Fruit-Shape and Peel-Colour Genes in Wax Gourd

Development of high-lycopene tomato hybrids using conventional breeding techniques and molecular markers

Analysis of SlMYB12 gene polymorphism determining chalcone-naringenin biosynthesis in the skin of tomato fruits and its effect on the lycopene accumulation.

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