Single Nucleotide Polymorphisms of <i>Helicobacter pylori dup</i>A that Lead to Premature Stop Codons

Moura, Sílvia B.; Costa, Rafael; Anacleto, Charles; Rocha, Gifone Aguiar; Rocha, Andréia Maria Camargos; Queiroz, Dulciene Maria Magalhães

doi:10.1111/j.1523-5378.2011.00933.x

Cited by 11 publications

(12 citation statements)

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“…In our study, we have found the rate of frameshift mutations along the length of dupA gene was [34% (17/50)] high in the Indian population. Similar trend has also been observed in other populations [ 19 , 20 , 24 ]. On the other hand, 66% (33/50) of the dupA positive strains had no stop codon and were considered as intact dupA known as dupA1 .…”

Section: Discussionsupporting

confidence: 90%

“…However, the role of dupA as a virulence marker is still debated [ 9 - 17 ]. It has been reported that dupA gene is highly polymorphic as frameshift mutation found along the length of the gene that leads to truncated protein and the rate of frameshift mutation varies geographically around the world [ 12 , 18 - 20 ]. Thus, mere detection of dupA gene by PCR is not adequate to characterize this variable gene.…”

Section: Introductionmentioning

confidence: 99%

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Association of Intact dupA (dupA1) rather than dupA1 cluster with duodenal ulcer in Indian population

et al. 2015

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BackgroundThe duodenal ulcer promoting gene (dupA) and dupA cluster in Helicobacter pylori have been described as a risk factor for duodenal ulcer development in some populations. Polymorphic gene dupA can be divided into two groups, intact dupA1 (long or short type based on the presence or absence of 615-bp extra sequences at the 5′ region) having complete reading frame and other truncated dupA2 having frame-shift mutation. This study was aimed to elucidate the role of dupA of H. pylori and their clusters in the disease manifestation of Indian population.MethodsA total of 170 H. pylori strains were screened for the presence of dupA, dupA alleles and dupA cluster by PCR and sequencing. Pro-inflammatory cytokine (IL-8) with different dupA variant H. pylori stimulated gastric epithelial cells (AGS cells) was measured by ELISA.ResultsA total of 50 strains (29.4%) were positive for dupA among the tested 170 strains. The prevalence of dupA1 in duodenal ulcer (DU) and non-ulcer dyspepsia (NUD) populations was found to be 25.5% (25/98) and 11.1% (8/72), respectively and 16.4% (28/170) of the tested strains had dupA1, cagA and vacAs1m1 positive. The distribution of long and short type dupA1 has not been significantly associated with the disease outcome. The dupA cluster analysis showed that 10.2% (10/98) and 8.3% (6/72) strains were positive among DU and NUD, respectively. IL-8 production was significantly higher in dupA1+, cagA+, vacA+ (902.5 ± 79.01 pg/mL) than dupA2+, cagA+, vacA+ (536.0 ± 100.4 pg/mL, P = 0.008) and dupA−, cagA+, vacA+ (549.7 ± 104.1 pg/mL, P = 0.009). Phylogenetic analysis of dupA indicated that the Indian H. pylori strains clustered with East Asian strains but distinct from Western strains. This is the first known genetic element of Indian H. pylori that is genetically closer to the East Asian strains but differed from the Western strains.ConclusionsThe intact dupA1 was significantly associated with DU than NUD (P = 0.029) but the dupA1 cluster has no role in the disease manifestation at India (P = 0.79). Thus, dupA1 can be considered as a biomarker for DU patients in India.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Association of Intact dupA (dupA1) rather than dupA1 cluster with duodenal ulcer in Indian population

et al. 2015

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“…The Family Helicobacteraceae association with an increased risk of developing duodenal ulcer and protection against gastric atrophy, intestinal metaplasia and gastric carcinoma (Lu et al 2005;Moura et al 2012). However, as observed for other H. pylori virulence markers, the association between dupA and disease is dependent on the geographic origin of the H. pylori strains (Argent et al 2004;Hussein et al 2008;Schmidt et al 2009).…”

Section: Helicobacter Pylorimentioning

confidence: 94%

The Family Helicobacteraceae

et al. 2014

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“…By assigning strains with intact genes only as dup A‐positive, Moura et al. could demonstrate its association with DU in a Brazilian population and Queiroz et al. provided further evidence in support of dup A association with decreased risk of gastric cancer.…”

Section: Bacterial Virulence Factorsmentioning

confidence: 98%

Pathogenesis of Helicobacter pylori Infection

Delahay

Rugge

2012

Helicobacter

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Although Helicobacter pylori infection is highly prevalent in the global human population, the majority of infected individuals remain asymptomatic. A complex combination of host, environmental, and bacterial factors are considered to determine susceptibility and severity of outcome in the subset of individuals that develop clinical disease. These factors collectively determine the ability of H. pylori to colonize the gastric mucosa and profoundly influence the nature of the interaction that ensues. Many studies over the last year provide new insight into H. pylori virulence strategies and the activities of critical bacterial determinants that modulate the host environment. These latter include the secreted proteins CagA and VacA and adhesins BabA and OipA, which directly interact with host tissues. Observations from several studies extend the functional repertoire of CagA and the cag type IV secretion system in particular, providing further mechanistic understanding of how these important determinants engage and activate host signalling pathways important in the development of disease.

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Single Nucleotide Polymorphisms of Helicobacter pylori dupA that Lead to Premature Stop Codons

Cited by 11 publications

References 11 publications

Association of Intact dupA (dupA1) rather than dupA1 cluster with duodenal ulcer in Indian population

Association of Intact dupA (dupA1) rather than dupA1 cluster with duodenal ulcer in Indian population

The Family Helicobacteraceae

Pathogenesis of Helicobacter pylori Infection

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