Single Nucleotide Polymorphisms of Toll-Like Receptor 7 and Toll-Like Receptor 9 in Hepatitis C Virus Infection Patients from Central China

Abstract

PurposeTo analyze the correlation of polymorphisms of toll-like receptor 7 (TLR7) (rs179009) and toll-like receptor 9 (TLR9) (rs187084) in hepatitis C virus (HCV) infections in the Han population.Materials and MethodsThe genotypes of TLR7IVS2-151 in HCV infection were detected by Sanger sequencing using polymerase chain reaction-restriction fragment length polymorphism to determine the TLR9 T-1486C single nucleotide polymorphisms (SNP) for all enrolled patients.ResultsWe found no significant difference between males with spontaneous clearance of HCV versus those chronically infected [χ2=2.71, p=0.10, odd ratios (OR)=0.58, 95% confidence interval (CI) 0.31-1.11]. However, significant differences were found for the distribution of TLR7 (rs179009) in females (χ2=9.46, p=0.01). In females, a significant difference was also found between chronic hepatitis C and those with spontaneous clearance of HCV in terms of TLR7 IVS2-151G/A allele frequencies (χ2=9.50, p=0.00, OR=0.46, 95% CI 0.28-0.75). In HCV-infected patients, no significant association was found between the frequency of TLR9 genotypes and alleles.ConclusionThe site of TLR7 IVS2-151 (rs179009) G/A may be a factor for susceptibility of chronic HCV in the female Han population. TLR9T-1486C (rs18084) SNP may not play a major role in HCV infection. However, individual risk profiles for HCV infection did vary by sex and this relationship should be further investigated.

Sex-specific effects of TLR9 promoter variants on spontaneous clearance of HCV infection

“…Although Wei et al 40 conducted a similar study in chronically infected Chinese patients with HCV, they did not observe an association which may be due to their small cohort size.…”

Association of Toll-Like Receptor 3 and Toll-Like Receptor 9 Single Nucleotide Polymorphisms with Hepatitis C Virus Infection and Hepatic Fibrosis in Egyptian Patients

“…Similarly, Wei and others showed no significant association with regard to TLR-9 (rs187084) genotype and allele frequency between chronic HCV patients and subjects who spontaneously cleared the virus. 38 In humans, TLR-3-promoter region is responsible for maintenance of promoter integrality and promoter-specific virus responsive element. 39 In our study, the TLR-3 (_7 C/A) SNP within the promoter region is not associated with HCV infection, and our results are comparable to results in other ethnicities.…”

Toll-like receptor 7 Gln11Leu, c.4-151A/G, and +1817G/T polymorphisms in Crimean Congo hemorrhagic fever

“…However, patients with fatal cases of CCHF have a 7.63‐fold greater frequency of the GG genotype of TLR7 c.4‐151 A/G polymorphism (Table ). Wei et al [] found that the relationship between the TLR7 c.4‐151 A/G polymorphism and patients infected with HCV (hepatitis C virus) is dependent on gender. In one study it was revealed that TLR 7 has a considerable role in HCV infection because ssRNAs can be bound by TLR 7, giving rise to the production of high levels of the antiviral cytokine interferon‐α by DC [Colonna et al, ].…”