Single nucleotide polymorphisms reveal multiple introductions into France of Plasmopara halstedii, the plant pathogen causing sunflower downy mildew

Delmotte, François; Giresse, Xavier; Richard-Cervera, S.; M’Baya, Jessica; Vear, Félicity; Tourvieille, Jeanne; Walser, Pascal; Labrouhe, Denis Tourvieille de

doi:10.1016/j.meegid.2008.02.012

Cited by 86 publications

(111 citation statements)

References 39 publications

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“…Delmotte et al (2008) and Ahmed et al (2012) grouped race 304 a genetic clade and race 314 in another cluster; however, this association was identified in the present work. Delmotte et al (2008) and Ahmed et al (2012) grouped races 704 and 714 together in the same genetic clade; however, this association was not identified in the present work and in data by Sakr (2011aSakr ( , 2014bSakr ( , 2014c. Our results underlined non correlation between EST genotypes (Table 5 and Fig.…”

Section: Discussioncontrasting

confidence: 82%

“…The 12 EST-derived markers were used because the other molecular markers were non-specific, insufficiently polymorphic within P. halstedii and no genetic structure in P. halstedii populations was identified by using these markers (Delmotte et al, 2008). For 20 single zoosporangium isolates tested, DNA was isolated from infected plant tissue then the 12 polymorphic EST-derived markers (Delmotte et al, 2008) were used to genotype P. halstedii single zoosporangium isolates.…”

Section: Dna Extraction and Molecular Typingmentioning

confidence: 99%

“…For 20 single zoosporangium isolates tested, DNA was isolated from infected plant tissue then the 12 polymorphic EST-derived markers (Delmotte et al, 2008) were used to genotype P. halstedii single zoosporangium isolates. The polygenetic relations between the 20 isolates were obtained by building a Neighbour-joining (NJ) tree (Jin and Chakraborty, 1993) using Populations 1.2.28 Software (Librado and Rozas, 2009).…”

Section: Dna Extraction and Molecular Typingmentioning

confidence: 99%

“…The interactions between sunflower plants and P. halstedii on the level of pathogenicity have been the object of several studies: virulence and aggressiveness (Tourvieille de Labrouhe et al, 2000, 2010Delmotte et al, 2008;Sakr, 2011aSakr, , 2011bSakr, , 2011cSakr, , 2012Sakr, , 2014aSakr, , 2014bSakr, , 2014cSakr, , 2015aSakr, , 2015bAhmed et al, 2012). Virulence has been defined as specific disease-causing abilities and aggressiveness as non-specific disease-causing abilities ( Van der Plank, 1968).…”

mentioning

confidence: 99%

“…It displays a gene-for-gene interaction with its host plant and shows physiological races (pathotypes) capable of infecting a variable range of sunflower genotypes. Indeed, it has been possible to identify up to 35 races, with different virulence patterns (Delmotte et al, 2008;Ahmed et al, 2012;Sakr, 2014a). To date, the genetic background for avirulence Avr genes in P. halstedii correspondent to Pl sunflower resistance genes has not been investigated (Virányi and Spring, 2011;Sakr, 2015a).…”

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confidence: 99%

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Can Pl Resistance Gene in Sunflower Line D4 be Used to Differentiate the Pathogenicity in Four Plasmopara halstedii (Sunflower Downy Mildew) Races 304, 314, 704 and 714?

Sakr¹

2015

Acta Phytopathologica et Entomologica Hungarica

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In order to clarify the role of Pl PMI3 resistance gene in sunflower differential lines D4 for differentiation the pathogenicity in Plasmopara halstedii (sunflower downy mildew), analyses were carried out in two groups including four pathotypes which overcome and do not overcome Pl PMI3 (Pl gene has still not been mapped) resistance gene. Based on the reaction for the P. halstedii isolates to sunflower hybrids varying only in Pl resistance genes, there were no virulence differences for the two groups. Index of aggressiveness was calculated for pathogen isolates and revealed the presence of significant differences between isolates of races 304 and 314; however, there were no aggressiveness differences for 7xx races. Regarding the life-history trait and the genetic architecture of the pathogen: there were morphological and genetic variations for the four P. halstedii isolates without a correlation with pathogenic diversity. The importance of the Pl PMI3 resistance gene to differentiate the pathogenicity in sunflower downy mildew was discussed.

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