Single Nucleotide Polymorphisms within the Bovine DLK1-DIO3 Imprinted Domain Are Associated with Economically Important Production Traits in Cattle

Magee, David A.; Berry, D.P.; Berkowicz, E. W.; Sikora, K. M.; Howard, Dawn J.; Mullen, Michael P.; Evans, R.D.; Spillane, Charles; MacHugh, David E.

doi:10.1093/jhered/esq097

Cited by 29 publications

(20 citation statements)

References 38 publications

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“…In transgenic mice, skeletal muscle DLK1 is overexpressed, caused hypertrophy traits and affected muscle growth; similarly, a double muscled trait was caused in sheep (Davis et al, 2004;Magee et al, 2011;Bi and Kuang, 2012). In the present study, DLK1 expression was higher in the longissimus dorsi of males than in females at different growth stages after birth.…”

Section: Discussionsupporting

confidence: 55%

Association between DLK1 and IGF-I gene expression and meat quality in sheep

Su¹,

Sun²,

Li³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The aim of the present study was to detect delta-like 1 homolog (DLK1) and insulin-like growth factor-I (IGF-I) gene expression in the longissimus dorsi of Hu sheep at different growth stages and study the association between these genes and meat quality. The diameter and density of muscle fibers and tenderness of the longissimus dorsi were measured. Growth stage, but not sex, significantly affected DLK1 and IGF-I expression. DLK1 and IGF-I expression in the sheep longissimus dorsi gradually increased with growth, but also decreased during some periods. These results suggest that different growth stages significantly affect DLK1 and IGF-I gene expression in sheep muscle tissue. The expression of DLK1 and IGF-I genes were positively and significantly (P < 0.01) correlated with muscle fiber diameter and muscle fiber shear stress, and negatively and significantly (P < 0.01) correlated with muscle fiber density. Muscle fiber diameter was positively and significantly (P < 0.01) correlated with muscle fiber shear stress, and negatively and significantly (P < 0.01) correlated with muscle fiber density. In addition,

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Section: Discussionsupporting

confidence: 55%

Association between DLK1 and IGF-I gene expression and meat quality in sheep

Su¹,

Sun²,

Li³

et al. 2014

Genet. Mol. Res.

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“…The basis for this non-Mendelian effect on intrauterine growth remains unexplained, but increasing evidence strongly suggest that epigenetic inheritance involving imprinted genes is in play (Duselis et al, 2005; Jiang et al, 2007; Loschiavo et al, 2007; Cheverud et al, 2008; Hager et al, 2008; Wolf et al, 2008). Recently, a handful of known and putative imprinted genes have reportedly been associated with quantitative traits in beef cattle (Magee et al, 2010, 2011; Berkowicz et al, 2011; Chen et al, 2011; Sikora et al, 2011). …”

Section: Introductionmentioning

confidence: 99%

Genome Scan for Parent-of-Origin QTL Effects on Bovine Growth and Carcass Traits

Imumorin¹,

Kim²,

Lee³

et al. 2011

Front. Gene.

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Parent-of-origin effects (POE) such as genomic imprinting influence growth and body composition in livestock, rodents, and humans. Here, we report the results of a genome scan to detect quantitative trait loci (QTL) with POE on growth and carcass traits in Angus × Brahman cattle crossbreds. We identified 24 POE–QTL on 15 Bos taurus autosomes (BTAs) of which six were significant at 5% genome-wide (GW) level and 18 at the 5% chromosome-wide (CW) significance level. Six QTL were paternally expressed while 15 were maternally expressed. Three QTL influencing post-weaning growth map to the proximal end of BTA2 (linkage region of 0–9 cM; genomic region of 5.0–10.8 Mb), for which only one imprinted ortholog is known so far in the human and mouse genomes, and therefore may potentially represent a novel imprinted region. The detected QTL individually explained 1.4 ∼ 5.1% of each trait’s phenotypic variance. Comparative in silico analysis of bovine genomic locations show that 32 out of 1,442 known mammalian imprinted genes from human and mouse homologs map to the identified QTL regions. Although several of the 32 genes have been associated with quantitative traits in cattle, only two (GNAS and PEG3) have experimental proof of being imprinted in cattle. These results lend additional support to recent reports that POE on quantitative traits in mammals may be more common than previously thought, and strengthen the need to identify and experimentally validate cattle orthologs of imprinted genes so as to investigate their effects on quantitative traits.

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“…Most of the known imprinted genes were identified in mice and humans, and studies in other organisms primarily have confirmed the imprinting status of previously identified genes (12,13). However, studies in domestic livestock have revealed variation in normal phenotypes associated with imprinted genes for muscle growth and insulin-like growth factor-2 (IGF2) in pigs (14), for the delta-like 1 homolog (DLK) region in sheep (15), and for production traits in dairy cattle (16). In an investigation of horse, donkey, mule, and hinny pregnancies, peak serum concentrations of the placental hormone equine chorionic gonadotropin (eCG) were markedly higher when the sire was a horse than in pregnancies in which the sire was a donkey (17).…”

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confidence: 99%

Paternally expressed genes predominate in the placenta

Wang

Miller

Harman

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

154

143

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The discovery of genomic imprinting through studies of manipulated mouse embryos indicated that the paternal genome has a major influence on placental development. However, previous research has not demonstrated paternal bias in imprinted genes. We applied RNA sequencing to trophoblast tissue from reciprocal hybrids of horse and donkey, where genotypic differences allowed parent-of-origin identification of most expressed genes. Using this approach, we identified a core group of 15 ancient imprinted genes, of which 10 were paternally expressed. An additional 78 candidate imprinted genes identified by RNA sequencing also showed paternal bias. Pyrosequencing was used to confirm the imprinting status of six of the genes, including the insulin receptor ( INSR ), which may play a role in growth regulation with its reciprocally imprinted ligand, histone acetyltransferase-1 ( HAT1 ), a gene involved in chromatin modification, and lymphocyte antigen 6 complex, locus G6C, a newly identified imprinted gene in the major histocompatibility complex. The 78 candidate imprinted genes displayed parent-of-origin expression bias in placenta but not fetus, and most showed less than 100% silencing of the imprinted allele. Some displayed variability in imprinting status among individuals. This variability results in a unique epigenetic signature for each placenta that contributes to variation in the intrauterine environment and thus presents the opportunity for natural selection to operate on parent-of-origin differential regulation. Taken together, these features highlight the plasticity of imprinting in mammals and the central importance of the placenta as a target tissue for genomic imprinting.

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Single Nucleotide Polymorphisms within the Bovine DLK1-DIO3 Imprinted Domain Are Associated with Economically Important Production Traits in Cattle

Cited by 29 publications

References 38 publications

Association between DLK1 and IGF-I gene expression and meat quality in sheep

Association between DLK1 and IGF-I gene expression and meat quality in sheep

Genome Scan for Parent-of-Origin QTL Effects on Bovine Growth and Carcass Traits

Paternally expressed genes predominate in the placenta

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