Single-nucleus profiling of adult mice sub-ventricular zone after blast-related traumatic brain injury

Li, Manrui; Chen, Xiameng; Yang, Qixiang; Cao, Shuqiang; Wyler, Steven C.; Yuan, Ruixuan; Zhang, Lingxuan; Liao, Miao; Lv, Meili; Wang, Feng; Guo, Yadong; Zhou, Jihong; Zhang, Lin; Xie, Xiaoqi; Liang, Weibo

doi:10.1038/s41597-022-01925-y

Cited by 3 publications

(2 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Expression matrixes were loaded into R (v.4.1.3) using the function Read10× in Seurat (v.1.1) ( Stuart et al 2019 ; R Core Team 2023 ) and then merged together by column (detailed data set descriptions of the sham group have been reported in our recent data descriptor) ( Li et al 2023 ; ). This resulted in a total of 16,754 cells.…”

Section: Methodsmentioning

confidence: 99%

Single-nucleus transcriptomic mapping uncovers targets for traumatic brain injury

Yang,

Zhang,

et al. 2023

Genome Res.

Self Cite

View full text Add to dashboard Cite

The subventricular zone (SVZ) is a neurogenic niche that contributes to homeostasis and repair after brain injury. However, the effects of mild traumatic brain injury (mTBI) on the divergence of the regulatory DNA landscape within the SVZ and its link to functional alterations remain unexplored. In this study, we meticulously mapped the transcriptome atlas of murine SVZ and its responses to mTBI at the single-cell level. We observed cell-specific gene expression changes following mTBI and unveiled diverse cell-to-cell interaction networks that influence a wide array of cellular processes. Moreover, we reported novel neurogenesis lineage trajectories and related key transcription factors, which we subsequently validated through loss-of-function experiments. Specifically, we validated the role ofTcf7l1, a cell cycle gene regulator, in promoting neural stem cell differentiation towards the neuronal lineage after mTBI, providing a potential target for regenerative medicine. Overall, our study profiles an SVZ transcriptome reference map, which underlies the differential cellular behavior in response to mTBI. The identified key genes and pathways that may ameliorate brain damage or facilitate neural repair serve as valuable resources for drug discovery in the context of mTBI.

show abstract

Section: Methodsmentioning

confidence: 99%

Single-nucleus transcriptomic mapping uncovers targets for traumatic brain injury

Yang,

Zhang,

et al. 2023

Genome Res.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Currently, two points of Neurogenesis in the brain are well known: the subventricular zone (SVZ), adjacent to the lateral ventricles, and the subgranular zone (SGZ) of the dentate gyrus (DG) of the hippocampus ( Altman and Das, 1966 ; Altman, 1969a , b ; Altmann et al, 2019 ). In addition, according to some data, Neurogenesis can also occur in the hippocampus, cerebral cortex, striatum, amygdala, and substantia nigra after brain damage ( Gould et al, 2000 ; Li et al, 2023 ; Woitke et al, 2023 ; Yang J.L. et al, 2023 ).…”

Section: Targeting Adult Neurogenesis To Enhance Memory Abilities Usi...mentioning

confidence: 99%

Nicotinic acetylcholine receptors and learning and memory deficits in Neuroinflammatory diseases

2023

View full text Add to dashboard Cite

Animal survival depends on cognitive abilities such as learning and memory to adapt to environmental changes. Memory functions require an enhanced activity and connectivity of a particular arrangement of engram neurons, supported by the concerted action of neurons, glia, and vascular cells. The deterioration of the cholinergic system is a common occurrence in neurological conditions exacerbated by aging such as traumatic brain injury (TBI), posttraumatic stress disorder (PTSD), Alzheimer’s disease (AD), and Parkinson’s disease (PD). Cotinine is a cholinergic modulator with neuroprotective, antidepressant, anti-inflammatory, antioxidant, and memory-enhancing effects. Current evidence suggests Cotinine’s beneficial effects on cognition results from the positive modulation of the α7-nicotinic acetylcholine receptors (nAChRs) and the inhibition of the toll-like receptors (TLRs). The α7nAChR affects brain functions by modulating the function of neurons, glia, endothelial, immune, and dendritic cells and regulates inhibitory and excitatory neurotransmission throughout the GABA interneurons. In addition, Cotinine acting on the α7 nAChRs and TLR reduces neuroinflammation by inhibiting the release of pro-inflammatory cytokines by the immune cells. Also, α7nAChRs stimulate signaling pathways supporting structural, biochemical, electrochemical, and cellular changes in the Central nervous system during the cognitive processes, including Neurogenesis. Here, the mechanisms of memory formation as well as potential mechanisms of action of Cotinine on memory preservation in aging and neurological diseases are discussed.

show abstract

The role of astrocyte in neuroinflammation in traumatic brain injury

He,

Zhang,

Yang

et al. 2024

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

View full text Add to dashboard Cite

Single-nucleus profiling of adult mice sub-ventricular zone after blast-related traumatic brain injury

Cited by 3 publications

References 31 publications

Single-nucleus transcriptomic mapping uncovers targets for traumatic brain injury

Single-nucleus transcriptomic mapping uncovers targets for traumatic brain injury

Nicotinic acetylcholine receptors and learning and memory deficits in Neuroinflammatory diseases

The role of astrocyte in neuroinflammation in traumatic brain injury

Contact Info

Product

Resources

About