Single-quantum-dot-based DNA nanosensor

Zhang, Chun Yang; Yeh, Hsin Chih; Kuroki, Marcos T.; Wang, Tza Huei

doi:10.1038/nmat1508

Cited by 920 publications

(501 citation statements)

References 30 publications

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“…1-5 These properties make QDs powerful tools for labeling and optical imaging in biological, 3,6-11 biomedical, 12-18 and chem-/bio-sensing applications. [19][20][21][22] Imaging cellular events at the single-molecule level is a particular area where the superior brightness and photostability of QDs excel as compared to more typical dye and fluorescent protein imaging reagents. 11 Notwithstanding, the optimal design of QDs for single-molecule imaging in live cells presents a unique set of challenges.…”

Section: Introductionmentioning

confidence: 99%

Compact Biocompatible Quantum Dots Functionalized for Cellular Imaging

et al. 2008

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We present a family of water-soluble quantum dots (QDs) that exhibit low nonspecific binding to cells, small hydrodynamic diameter, tunable surface charge, high quantum yield, and good solution stability across a wide pH range. These QDs are amenable to covalent modification via simple carbodiimide coupling chemistry, which is achieved by functionalizing the surface of QDs with a new class of heterobifunctional ligands incorporating dihydrolipoic acid, a short poly(ethylene glycol) (PEG) spacer, and an amine or carboxylate terminus. The covalent attachment of molecules is demonstrated by appending a rhodamine dye to form a QD-dye conjugate exhibiting fluorescence resonance energy transfer (FRET). High-affinity labeling is demonstrated by covalent attachment of streptavidin, thus enabling the tracking of biotinylated epidermal growth factor (EGF) bound to EGF receptor on live cells. In addition, QDs solubilized with the heterobifunctional ligands retain their metal-affinity driven conjugation chemistry with polyhistidine-tagged proteins. This dual functionality is demonstrated by simultaneous covalent attachment of a rhodamine FRET acceptor and binding of polyhistidine-tagged streptavidin on the same nanocrystal to create a targeted QD, which exhibits dual-wavelength emission. Such emission properties could serve as the basis for ratiometric sensing of the cellular receptor's local chemical environment.

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Section: Introductionmentioning

confidence: 99%

Compact Biocompatible Quantum Dots Functionalized for Cellular Imaging

et al. 2008

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“…Quantum dots modified with streptavidin are then added which bind the biotin moiety thereby generating the FRET (C. Y. Zhang, Yeh, Kuroki, & Wang, 2005). The complex thereby emits light through photobioluminescence and FRET thereby generating an intense signal.…”

Section: Genosensorsmentioning

confidence: 99%

“…The complex is illuminated with light (488 nm) that induces photoluminescence and FRET of the QD and Cy5 respectively (b). The different wavelength emitted light is detected via a microfluid-type device with illumination using an argon laser (488 nm) using duel photodiodes (c) (Reprinted with permission from Zhang et al, 2005).…”

Section: Genosensorsmentioning

confidence: 99%

Developments in nanoparticles for use in biosensors to assess food safety and quality

Warriner

Reddy

Namvar

et al. 2014

Trends in Food Science & Technology

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Highlights•Background to the evolution of biosensor based on nanoparticles.•Application of nanoparticles in biosensor devices.•Overview of biosensor formats applied in food testing.•Examples of integrated biosensor systems for food sampling.•Prospects and challenges in nanosensors in pathogen testing.The following will provide an overview on how advances in nanoparticle technology have contributed towards developing biosensors to screen for safety and quality markers associated with foods. The novel properties of nanoparticles will be described and how such characteristics have been exploited in sensor design will be provided. All the biosensor formats were initially developed for the health care sector to meet the demand for point-of-care diagnostics. As a consequence, research has been directed towards miniaturization thereby reducing the sample volume to nanolitres. However, the needs of the food sector are very different which may ultimately limit commercial application of nanoparticle based nanosensors. IntroductionThrough evolution we have developed senses to enable us to differentiate good foods from bad. The art of cheese making, brewing and viticulture has relied on the producer using their senses to assess quality. Establishing if a food was safe was more problematic and typically relied on tasters to ensure dishes provided to their masters were sound. Clearly, analytical testing is a more reliable and objective means of assessing the quality and safety of foods. Laboratory testing provided a basis for developing protocols and limits that could be applied to foods to define quality or safety. Yet, the expense and delay in acquiring results remain critical limitations. Consequently, there is an on-going demand for biosensors that can be used outside the laboratory environment to assess the safety and quality of foods on-site.In many instances the needs of the food industry mirrored that of the health sector where rapid diagnostics would enhance treatments and prognosis. The health sector provides a more lucrative market for diagnostic companies so it is not unexpected to find that the majority of research to date has been towards developing biosensors to meet the market demands. Consequently, those biosensors encountered in the food sector are derived from those devices initially fabricated to meet the needs of the health care sector. Within this context, the following review provides a brief history on how the field of biosensors developed and the contribution made by advances in nanoparticle technology. The efforts in transferring biosensor technologies to the food sector will be provided with a focus on safety, and to a degree, quality assessment. The review does not attempt to provide an exhaustive list of advances given the numerous quality reviews are already published in this area (Arugula and Simonian,

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“…Facile and reliable methods for detection of DNA are of vital importance to medical diagnosis, mutational analysis, gene therapy, biological studies, and specific genomic techniques 1, 2, 3. The innovative assays for DNA detection by conjugated polyelectrolytes (CPEs) have been reported recently, which combine the unique optical amplification properties of π‐conjugated polymers and the electrostatic behaviors of polyelectrolytes 4, 5, 6.…”

Section: Introductionmentioning

confidence: 99%

Conjugated Polymer Nanoparticles for Label‐Free and Bioconjugate‐Recognized DNA Sensing in Serum

Bao

Zai

et al. 2015

Advanced Science

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Hybridbio/‐synthetic sensory conjugated polymer nanoparticles (CPNs) are developed for selective label‐free detection of target ssDNA in serum. Carboxylic acid‐functionalized anionic polyfluorene nanoparticles are rationally designed as signal amplifying unit to bioconjugate with amine functionalized single stranded oligonucleotides as a receptor. The covalent DNA coating can signiﬁcantly improve the photostability of the DNA‐bioconjugated CPNs over a wide range of buffer conditions. Better ssDNA discrimination for the DNA‐bioconjugated CPNs sensor is achieved owing to increased interchain interactions and more efficient exciton transport in nanoparticles. The distinguishable ﬂuorescent color for DNA‐bioconjugated CPNs in the presence of target ssDNA allows naked‐eye detection of ssDNA under UV irradiation.

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Single-quantum-dot-based DNA nanosensor

Cited by 920 publications

References 30 publications

Compact Biocompatible Quantum Dots Functionalized for Cellular Imaging

Compact Biocompatible Quantum Dots Functionalized for Cellular Imaging

Developments in nanoparticles for use in biosensors to assess food safety and quality

Conjugated Polymer Nanoparticles for Label‐Free and Bioconjugate‐Recognized DNA Sensing in Serum

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