Single-Shot 3D Topography of Transmissive and Reflective Samples with a Dual-Mode Telecentric-Based Digital Holographic Microscope

Abstract

Common path DHM systems are the most robust DHM systems as they are based on self-interference and are thus less prone to external fluctuations. A common issue amongst these DHM systems is that the two replicas of the sample’s information overlay due to self-interference, making them only suitable for imaging sparse samples. This overlay has restricted the use of common-path DHM systems in material science. The overlay can be overcome by limiting the sample’s field of view to occupy only half of the imaging field of view or by using an optical spatial filter. In this work, we have implemented optical spatial filtering in a common-path DHM system using a Fresnel biprism. We have analyzed the optimal pinhole size by evaluating the frequency content of the reconstructed phase images of a star target. We have also measured the accuracy of the system and the sensitivity to noise for different pinhole sizes. Finally, we have proposed the first dual-mode common-path DHM system using a Fresnel biprism. The performance of the dual-model DHM system has been evaluated experimentally using transmissive and reflective microscopic samples.

Image Processing Techniques for Improving Quality of 3D Profile in Digital Holographic Microscopy Using Deep Learning Algorithm

“…Digital holographic microscopy (DHM) is the application of DH in the field of microscopy. Unlike traditional microscopes, DHM offers the capability of 3D imaging without the need for physical sectioning [ 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ]. It achieves rapid and non-destructive 3D reconstruction of samples through the image processing of holograms.…”

“…It achieves rapid and non-destructive 3D reconstruction of samples through the image processing of holograms. This technology is particularly well-suited for fields such as biomedical research [ 7 ], materials science [ 8 ], and microelectronics [ 9 ]. It enables the observation of details in the microscopic world, including living cells, micro-nano structures, and material surfaces [ 10 ].…”