Based on the needs of detection and calibration with low-energy X-ray, we used the relationship characteristics of X-ray fluorescence, absorption and limit potential target excitation, developed the X-ray device with adjustable intensity (single photon to 10 7 /s), optional energy points (4 keV-20 keV), highly portable (≤1 kg), by the matching design of fluorescence energy conversion target and modulation means, coupled transport simulation of electron-photon in target, meanwhile, we solved the low-energy X-ray radiation field diagnose problems with HPGe detector, which calibrated with combined technique, including relatively wide energy efficiency simulation and single energy point of absolute efficiency calibration. In single-photon calibration field of soft X-ray pulsar navigation detect, the portable reference single energy radiation fields was applied effectively and got good result, the reference radiation fields provided perfect experiment means for the scientific study of pulsar navigation detecting, sun X-ray monitoring, etc.