Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron near Infrared Beam

Han, Molong; Smith, Daniel; Ng, Soon Hock; Katkus, Tomas; Rajeswary, Aravind Simon John Francis; Praveen, Periyasamy Angamuthu; Bambery, Keith R.; Vongsvivut, Jitraporn; Juodkazis, Saulius; Anand, Vijayakumar

doi:10.3390/bios12121073

Cited by 6 publications

(5 citation statements)

References 20 publications

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“…The proposed method is not limited only to applications in the FTIRm system of the Australian Synchrotron but can be applied to other advanced imaging systems with complicated optical configurations. Recently, single shot phase imaging has been demonstrated at the Australian Synchrotron [22]. Scanning holography is a milestone in the history of holography [10].…”

Section: Discussionmentioning

confidence: 99%

Computational Imaging at the Infrared Beamline of the Australian Synchrotron Using the Lucy–Richardson–Rosen Algorithm

Ng,

Anand,

Han

et al. 2023

Applied Sciences

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The Fourier transform infrared microspectroscopy (FTIRm) system of the Australian Synchrotron has a unique optical configuration with a peculiar beam profile consisting of two parallel lines. The beam is tightly focused using a 36× Schwarzschild objective to a point on the sample and the sample is scanned pixel by pixel to record an image of a single plane using a single pixel mercury cadmium telluride detector. A computational stitching procedure is used to obtain a 2D image of the sample. However, if the imaging condition is not satisfied, then the recorded object’s information is distorted. Unlike commonly observed blurring, the case with a Schwarzschild objective is unique, with a donut like intensity distribution with three distinct lobes. Consequently, commonly used deblurring methods are not efficient for image reconstruction. In this study, we have applied a recently developed computational reconstruction method called the Lucy–Richardson–Rosen algorithm (LRRA) in the online FTIRm system for the first time. The method involves two steps: training step and imaging step. In the training step, the point spread function (PSF) library is recorded by temporal summation of intensity patterns obtained by scanning the pinhole in the x-y directions across the path of the beam using the single pixel detector along the z direction. In the imaging step, the process is repeated for a complicated object along only a single plane. This new technique is named coded aperture scanning holography. Different types of samples, such as two pinholes; a number 3 USAF object; a cross shaped object on a barium fluoride substrate; and a silk sample are used for the demonstration of both image recovery and 3D imaging applications.

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Section: Discussionmentioning

confidence: 99%

Computational Imaging at the Infrared Beamline of the Australian Synchrotron Using the Lucy–Richardson–Rosen Algorithm

Ng,

Anand,

Han

et al. 2023

Applied Sciences

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“…From this recorded intensity distribution, the phase information has to be extracted which is carried out using the rapidly converging phase-retrieval algorithm as shown in Figure 2 [8][9][10]. There are two planes of interest namely sample plane and sensor plane with two complex amplitudes ψ1 and ψ2 respectively with their intensities either known or measurable i.e., Airy pattern from pinhole and diffraction pattern from the sample.…”

Section: Methodsmentioning

confidence: 99%

Three-dimensional phase imaging with near infrared synchrotron beam using phase-retrieval algorithm

Han

Smith

et al. 2023

Practical Holography XXXVII: Displays, Materials, and Applications

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The near infrared (NIR) part of the infrared synchrotron beam is usually dumped to improve the signal to noise ratio of spectral imaging. In this study, this NIR synchrotron beam has been extracted and used for three-dimensional (3D) phase imaging. A pinhole was inserted in the path of the fork shaped NIR synchrotron beam and the Airy diffraction pattern was aligned with biochemical samples and the diffracted intensity distribution was captured using an image sensor sensitive to NIR. A phase retrieval algorithm was used to estimate the 3D phase distribution at the object plane from the recorded intensity distribution.

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“…The fabricated devices were tested with the synchrotron IR beam using an optical configuration as shown in Figure 9 similar to a recently demonstrated phase imaging concept [44]. The IRM beamline at the Australian Synchrotron consists of two IR microscopes, one with Globar™ IR source and one with synchrotron IR beam with a high brightness.…”

Section: Experiments With Synchrotron Ir Beammentioning

confidence: 99%

Extending the Depth of Focus of Infrared Microscope Using a Binary Axicon Fabricated on Barium Fluoride

Han,

Smith,

Kahro

et al. 2024

Preprint

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Axial resolution is one of the most important characteristics of a microscope. In all microscopes, a high axial resolution is desired in order to discriminate information efficiently along the longitudinal direction. However, when studying thick samples that do not contain laterally overlapping information, a low axial resolution is desirable as information from multiple planes can be recorded simultaneously from a single camera shot instead of plane-by-plane mechanical refocusing. In this study, we increased the focal depth of an infrared microscope non-invasively by introducing a binary axicon fabricated on barium fluoride substrate close to the sample. Preliminary results of imaging thick and sparse silk fibers showed an improved focal depth with a slight decrease in lateral resolution and increase in background noise.

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Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron near Infrared Beam

Cited by 6 publications

References 20 publications

Computational Imaging at the Infrared Beamline of the Australian Synchrotron Using the Lucy–Richardson–Rosen Algorithm

Computational Imaging at the Infrared Beamline of the Australian Synchrotron Using the Lucy–Richardson–Rosen Algorithm

Three-dimensional phase imaging with near infrared synchrotron beam using phase-retrieval algorithm

Extending the Depth of Focus of Infrared Microscope Using a Binary Axicon Fabricated on Barium Fluoride

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