Although the most preferred treatment methods in cancer treatment are still surgery and chemotherapy, microwave ablation, one of the minimally invasive thermal ablation techniques, is increasingly used in the clinic for patients who cannot afford the risks of these treatment methods. In this study, microwave ablation with a new NiTi ring antenna was performed on a freshly slaughtered beef liver as an Ex Vivo application. Design and optimization was carried out in the CST Microwave studio. Ex Vivo MWA application was carried out at 2.45 GHz, using 50 W microwave power for 5 minutes. The lowest width of the ablation zone formed along the x-axis was 14.58 mm, the highest width was 28.61 mm, the length of the ablation area along the y-axis was 58.032 mm, and the area of the ablation zone was approximately 5.44 cm2. These results show that the proposed NiTi ring antenna has the ability to achieve a sufficient thermal lesion in terms of ablation zone dimensions.