Single-Step Fabrication of Gd ₂ O ₃ @SiO ₂ Nanoparticles for use as MRI Contrast Agents by Pulsed Laser Ablation in Liquid

Abstract

Gd2O3@SiO2 nanoparticles with a core-shell structure are synthesized by pulsed laser ablation in liquid (PLAL) in single steps. A Gd2O3 target immersed in tetraethyl orthosilicate (TEOS) is ablated by a microsecond Nd:YAG laser, which induces the generation of a Gd2O3 plasma plume and pyrolysis of the TEOS. We propose that the moment Gd2O3 nanoparticles are formed they will be coated immediately by SiO2 and directly synthesized Gd2O3@SiO2 core-shell nanoparticles. These particles obtain high 𝑟1 relaxivity of 5.26 s −1 mM −1 and are used as 𝑇1-weighted magnetic resonance imaging contrast agents. It is shown that the PLAL technique is promising for fabricating core-shell structure nanomaterial with potential medical applications.

Toxicity evaluation of Gd2O3@SiO2 nanoparticles prepared by laser ablation in liquid as MRI contrast agents in vivo

“…30 A Nd:YAG laser (wavelength λ=1,064 nm) with 6 µs pulse duration at 100 Hz was focused on the Gd 2 O 3 target. The ablated samples were collected by centrifuge to exclude large particles prior to further applications in vitro and in vivo.…”

“…Moreover, the dynamic enhancement curve of xenografted tumor and liver demonstrated a "fast-in and fast-out" pattern ( Figure 5B). 40 Based on our previous studies, 30,36,39 the optimized times for MRI imaging are a function of the probe and target tissue. Gd-DTPA quickly drops to baseline due to renal clearance.…”

“…[23][24][25][26] Characterization of the immune response to these agents is particularly relevant, 27,28 especially in vivo. [28][29][30] These studies are important to understand the relationship between NP structure and toxicity and thus provide the rational suggestions on the design of contrast agents in the future. 8 To the best of our knowledge, we present here the most extensive characterization of LAL NP in vivo toxicity, pharmacokinetics, and pharmacodynamics.…”

“…In our previous research, Gd-NPs have been successfully synthesized by LAL in a single step and shown to be sensitive MRI contrast agents. 30 However, their toxicity, pharmacokinetics, and in vivo utility have not yet been investigated. Here we report the systematic evaluation of Gd-NP toxicity including cell viability and apoptosis, reactive oxygen species (ROS) assays, changes to lymphocyte populations, serum cytokine levels, pharmacokinetics, and pharmacodynamics.…”

PROPERTIES OF MESOPOROUS AMORPHOUS-NANOCRYSTALLINE Gd2O3-SiO2 POWDERS PREPARED BY PULSED ELECTRON BEAM EVAPORATION

“…В частности, в отдельных работах показана возможность создавать биосовместимую оболочку из оксида кремния на поверхности наночастиц 23 Gd O [4][5][6]. Цель настоящей работы заключалась в получении и исследовании свойств мезопористых нанопорошков (НП) оксида гадолиния, покрытых биосовместимой оболочкой из оксида кремния, с помощью испарения импульсным электронным пучком на установке НАНОБИМ-2 [7] в вакууме (остаточное давление 4 Па).…”