Single-step fermentation of agricultural hemp residues for hydrogen and ethanol production

Agbor, Valery; Zurzolo, Francesco; Blunt, Warren; Dartiailh, Christopher; Cicek, Nazim; Sparling, Richard

doi:10.1016/j.biombioe.2014.03.027

Cited by 27 publications

(16 citation statements)

References 30 publications

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“…Differences in feedstock type and composition [ 6 – 8 ], time of harvest [ 3 , 9 ] and pretreatment strategies [ 10 , 11 ] have all been previously assessed in regard to C. thermocellum -mediated conversion to ethanol. However, little has been reported regarding the effect that substrate loading has on C. thermocellum solubilization and biofuel production despite the realization that feedstock loadings in excess of > 100 g/L carbohydrate are considered essential for industrialization and economic viability of cellulosic ethanol [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

The effect of switchgrass loadings on feedstock solubilization and biofuel production by Clostridium thermocellum

Verbeke¹,

García²,

Elkins³

2017

Biotechnol Biofuels

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BackgroundEfficient deconstruction and bioconversion of solids at high mass loadings is necessary to produce industrially relevant titers of biofuels from lignocellulosic biomass. To date, only a few studies have investigated the effect of solids loadings on microorganisms of interest for consolidated bioprocessing. Here, the effects that various switchgrass loadings have on Clostridium thermocellum solubilization and bioconversion are investigated.Results Clostridium thermocellum was grown for 10 days on 10, 25, or 50 g/L switchgrass or Avicel at equivalent glucan loadings. Avicel was completely consumed at all loadings, but total cellulose solubilization decreased from 63 to 37% as switchgrass loadings increased from 10 to 50 g/L. Washed, spent switchgrass could be additionally hydrolyzed and fermented in second-round fermentations suggesting that access to fermentable substrates was not the limiting factor at higher feedstock loadings. Results from fermentations on Avicel or cellobiose using culture medium supplemented with 50% spent fermentation broth demonstrated that compounds present in the supernatants from the 25 or 50 g/L switchgrass loadings were the most inhibitory to continued fermentation.ConclusionsRecalcitrance alone cannot fully account for differences in solubilization and end-product formation between switchgrass and Avicel at increased substrate loadings. Experiments aimed at separating metabolic inhibition from inhibition of hydrolysis suggest that C. thermocellum’s hydrolytic machinery is more vulnerable to inhibition from switchgrass-derived compounds than its fermentative metabolism.

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Section: Introductionmentioning

confidence: 99%

The effect of switchgrass loadings on feedstock solubilization and biofuel production by Clostridium thermocellum

Verbeke¹,

García²,

Elkins³

2017

Biotechnol Biofuels

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“…Alternatively, consolidated bioprocessing production (CBP) has been developed; in this process, cellulase production, enzymatic saccharification and fermentation can be accomplished in a single step by cellulolytic microorganisms. Hence, CBP is an attractive alternative due to its simpler operation, lower energy input and costs and higher conversion efficiency (Carere et al 2008b); hydrogen and ethanol co-production via CBP using lignocellulose and agricultural hemp residues, respectively, has been demonstrated (Agbor et al 2014;Ho et al 2011). …”

Section: Lignocellulosic Residuesmentioning

confidence: 98%

Mini review: hydrogen and ethanol co-production from waste materials via microbial fermentation

Soo

Yap

Hon

et al. 2015

World J Microbiol Biotechnol

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The simultaneous production of hydrogen and ethanol by microorganisms from waste materials in a bioreactor system would establish cost-effective and time-saving biofuel production. This review aims to present the current status of fermentation processes producing hydrogen accompanied by ethanol as a co-product. We outlined the microbes used and their fundamental pathways for hydrogen and ethanol fermentation. Moreover, we discussed the exploitation of renewable and sustainable waste materials as promising feedstock and the limitations encountered. The low substrate bioconversion rate in hydrogen and ethanol co-production is regarded as the primary constraint towards the development of large scale applications. Thus, microbes with an enhanced capability have been generated via genetic manipulation to diminish the inefficiency of substrate consumption. In this review, other potential approaches to improve the performance of co-production through fermentation were also elaborated. This review will be a useful guide for the future development of hydrogen and ethanol co-production using waste materials.

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“…Industrial hemp might be therefore one of the top solutions since energy can be produced through biomass production. [17,[20][21][22]. Due to the high biogas yield (3066 m 3 /ha), which is in the upper range of many other plant straws such as clover grass (2900-4000 m 3 /ha), industrial hemp is a fairly good opportunity for long term biogas production.…”

Section: Tetrahydrocannabinol (δ9-thc) [4]mentioning

confidence: 99%

“…The plants still being green, hemp is harvested for hempseeds when it begins to shatter. Nevertheless, about 70 per cent of seeds will be ripe at this time, and the moisture is about [22][23][24][25][26][27][28][29][30] When hemp is cultivated for its fibre, the harvesting usually takes part between early bloom and seed set, depending on the quality of in the stems harvesting with combine might cause problems as the fibres wrapping in the combine can cause After the hemp is cut, it must undergo a process called retting. The bonds between the two different types of fibres in the hemp plant, the bast (the outer long fibres) and the hurds (inner The most common process for that matter of fact is called field retting and involves leaving the crop in the field for up to five weeks to allow a field decomposition process to occur.…”

Section: Harvestingmentioning

confidence: 99%

Cannabis, the Plant of the Unlimited Possibilities

Dölle

Kurzmann

2019

AIR

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This review paper gives an overview of Cannabis Sativa, also known as hemp, which has been cultivated and used century's B.C. due to its medical, hallucinogenic and agricultural properties. Cannabis has been described in 1938 as the billion-dollar plant but has lost its value in the U.S. due to regulatory and legislative issues. Hemp has seen as new push In the U.S. with the introduction of the 2018 Farm Bill which allows on a federal level to grow hemp, pending on individual state regulations. Currently, industrial hemp production is allowed in at least 38 U.S. States under strict regulations. Today hemp is used in counties that do not have as strict regulations as the U.S. in a variety of applications such as beauty products, carpets, cooking oil, personal care products and textiles. Hemp can be considered as an alternative feedstock due to its low lignin and high cellulose level for biofuel application as an alternative to replace petroleum-based fuels and gases. In the U.S. hemp research in these areas has stalled due to the complexity of the law. Beside industrial application such as ropes, textiles, shoes, etc., hemp today is used in pharmaceutical and medical applications, by extracting Δ9-tetrahydrocannabinol and cannabinoids from the leaves and fluorescence of the hemp plant that contain no more than of up to 0.3% of these compounds. The use of hemp plants with higher levels are strictly forbidden in most countries. Several extraction processes of cannabinoids from hemp are used They all use a solvent for extraction but differ in terms of efficiency, usage range and other factors influencing the extraction. Nonetheless, overconsumption of cannabis products can be associated with several side effects, that can cause serious physiological and psychological damage in the human body may cause serious damage.

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Single-step fermentation of agricultural hemp residues for hydrogen and ethanol production

Cited by 27 publications

References 30 publications

The effect of switchgrass loadings on feedstock solubilization and biofuel production by Clostridium thermocellum

The effect of switchgrass loadings on feedstock solubilization and biofuel production by Clostridium thermocellum

Mini review: hydrogen and ethanol co-production from waste materials via microbial fermentation

Cannabis, the Plant of the Unlimited Possibilities

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