Single-step isolation method for six glycoforms of human α1-acid glycoprotein by hydroxylapatite chromatography and study of their binding capacities for disopyramide

Enantioselective Binding of Propranolol, Disopyramide, and Verapamil to Human α1‐Acid Glycoprotein

“…Kuroda et al 17 studied the binding of PL and VP enantiomers to AGP separated by concanavalin A lectin affinity chromatography and suggested the branching type of glycan chains of AGP does not play a significant role in the magnitude of the binding of both drugs and enantioselectivity. On the other hand, Kishino et al 18 studied the influence of glycans of AGP glycoforms, which were separated by hydroxyapatite chromatography, on the binding of DP and showed that the binding decreased with an increase in the extent of branching of the glycan chains. However, the ratio of genetic variants in these AGP glycoforms was not determined.…”

Relationship between human IgG structure and retention time in hydroxyapatite chromatography with sodium‐phosphate gradient elution

“…reported that various glycoforms of human serum alpha1-acid glycoprotein can be separated by phosphate gradient hydroxyapatite chromatography and that glycoforms with highly branched glycan chains tended to elute earlier than those with poorly branched glycan chains [38]. Furthermore, Melcher et al reported that among mutants of human lysozyme, the one with the highest carbohydrate content eluted first from hydroxyapatite [39].…”

Enantioselective binding of disopyramide to α 1 -acid glycoprotein and its variants

“…At least six glycoforms of AAG could be separated by hydroxyapatite chromatography. We previously reported that variants 1 and 2 have a significantly lower binding capacities than other variants [8].…”

“…More recently Herve et al [7] reported that DP binds to only one of the AAG proteins/variants (A variants). We previously reported the existence of at least six variants of AAG and the dierences in the binding activities of each AAG variant to racemic DP [8]. Variants 1 and 2 showed signi®cantly lower binding activities than other variants.…”

“…All other reagents were of the highest grade available commercially and were used without further puri®cation. The separation of AAG variants was carried out according to the method developed in this laboratory [8]. AAG (ca.…”