Single-step, paper-based concentration and detection of a malaria biomarker

Pereira, David Y.; Chiu, Ricky Y.T.; Zhang, Samantha C.L.; Wu, Benjamin M.; Kamei, Daniel T.

doi:10.1016/j.aca.2015.04.040

Cited by 49 publications

(28 citation statements)

References 20 publications

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“…A micellar aqueous two-phase system (APTS), composed of non-ionic Triton X-114 surfactant, was used for concentrating of biomarkers in a sample. Compared to a conventional LFA, this developed LFA demonstrated a 10-fold detection limit improvement [140].…”

Section: The Current Application Of Gold Nanoparticles Based Lfamentioning

confidence: 98%

Lateral flow assays: Principles, designs and labels

Bahadır

Sezgintürk

2016

TrAC Trends in Analytical Chemistry

500

315

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Section: The Current Application Of Gold Nanoparticles Based Lfamentioning

confidence: 98%

Lateral flow assays: Principles, designs and labels

Bahadır

Sezgintürk

2016

TrAC Trends in Analytical Chemistry

500

315

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“…Although several particle-based biomarker enrichment systems have emerged, most of them suffer from long assay times, need for a cold storage-chain, poor enrichment efficiencies, and most significantly, have not been shown to function in a whole blood matrix. 20–22 …”

Section: A Introductionmentioning

confidence: 99%

Magnetically-enabled biomarker extraction and delivery system: towards integrated ASSURED diagnostic tools

Bauer

Kimmel

Adams

et al. 2017

Analyst

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Diagnosis of asymptomatic malaria poses a great challenge to global disease elimination efforts. Healthcare infrastructure in rural settings cannot support existing state-of-the-art tools necessary to diagnose asymptomatic malaria infections. Instead, lateral flow immunoassays (LFAs) are widely used as a diagnostic tool in malaria endemic areas. While LFAs are simple and easy to use, they are unable to detect low levels of parasite infection. We have developed a field deployable Magnetically-enabled Biomarker Extraction And Delivery System (mBEADS) that significantly improves limits of detection for several commercially available LFAs. Integration of mBEADS with leading commercial Plasmodium falciparum malaria LFAs improves detection limits to encompass an estimated 95% of the disease reservoir. This user-centered mBEADS platform makes significant improvements to a previously cumbersome malaria biomarker enrichment strategy by improving reagent stability, decreasing the processing time 10-fold, and reducing the assay cost 10-fold. The resulting mBEADS process adds just three minutes and less than $0.25 to the total cost of a single LFA, thus balancing sensitivity and practicality to align with the World Health Organization’s ASSURED criteria for point-of-care (POC) testing.

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“…The limit of detection of the device for detection of P. falciparum is 2.9 ± 1.2 ng mL −1 . In order to reduce the limit of detection, a hybrid paper-based diagnostic device has been fabricated for the detection of a malaria biomarker, Plasmodium lactate dehydrogenase (pLDH) [59]. pLDH is a glycolytic enzyme which forms in the asexual stages of malaria parasites.…”

Section: Malariamentioning

confidence: 99%

“…Over the past few decades, a variety of paper-based devices have been developed for the diagnosis of malaria parasites [50,51,[54][55][56][57][58][59][60][61][62]]. An automated two-dimensional paper device has been fabricated for the detection of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) [54].…”

Section: Malariamentioning

confidence: 99%