Single-step production of autologous bovine platelet concentrate for clinical applications in cattle

Constant, Caroline; Desrochers, A.; Gagnon, Carl A.; Provost, Chantale; Nichols, Sylvain; Marchionatti, Emma; Gara-Boivin, Carolyn

doi:10.3168/jds.2021-21108

Cited by 4 publications

(7 citation statements)

References 45 publications

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“…This correlation confirms the naturally occurring defense reaction of the body triggered by cytokine release and observed in connection with stress, inflammation initiated at any time, including subclinical and clinical mastitis with an adequate increase in the number of somatic cells in milk [31]. Moreover, this dependence even may be used for therapeutic administration of bovine platelet concentrates in the treatment of mastitis or metritis in cattle [9].…”

Section: Discussionsupporting

confidence: 78%

Correlations between Selected Blood Markers of Hematological Profile and Milk Components in Dairy Cows

Mordak,

Popiel,

Dobrzański

et al. 2024

Preprint

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Clinical and laboratory diagnostics are particularly important in monitoring the internal homeostasis of the body in high-yielding animals, especially in dairy cows, whose metabolism is under constant pressure to obtain high parameters of milk production and reproduction. Knowledge of laboratory test results and relationships based on significant correlations between various milk and blood parameters may be very useful in veterinary diagnostics and helpful in cattle practice in order to protect animal health and the quality of milk production. The aim of the study was to analyze the correlation between selected hematological biomarkers such as white blood corpuscles (WBC), red blood corpuscles (RBC), hemoglobin concentration (HGB), hematocrit (HCT), platelets (PLT), erythrocyte mean corpuscular volume (MCV), mean corpuscular hemoglobin content (MHC), mean corpuscular hemoglobin concentration (MCHC) and selected quantitative-qualitative parameters of cow’s milk such as current milk production, somatic cells count (SCC), colony of bacteria forming units (CFU), milk fat (MF), milk protein (MP), milk lactose (ML), milk solids (S), solids non-fat in milk (SNF), daily milk production. Samples were collected three times at daily intervals from 11 clinically healthy late-lactation dairy cows obtaining 33 sets of test results. This repeated study of cows allowed for obtaining a homogeneous group in order to determine statistical correlations between selected blood and milk parameters. Many significant correlations were found between the examined parameters. In cows, significant positive correlations were recorded between milk production and HGB, HCT, MCV or MHC. Significant negative correlations were also found between SCC and HGB, SCC and HCT, SNF and MCV, SNF and MHC, and between MF and RBC.

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Section: Discussionsupporting

confidence: 78%

Correlations between Selected Blood Markers of Hematological Profile and Milk Components in Dairy Cows

Mordak,

Popiel,

Dobrzański

et al. 2024

Preprint

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“…While this is the most clinically relevant approach, it is possible that varying this protocol may result in greater or lesser stratification between leukocytes, erythrocytes, and platelets, producing a more heterogeneous PRP. Constant et al reported that an increased number of rotations per minute for a shorter period increased the platelet enrichment of the PRP sample without changing leukocyte or erythrocyte concentrations of the PRP when processing cow blood 17 . However, they pierced the buffy coat during aspiration in every sample, which further confounds comparison of the results with this study's results.…”

Section: Discussionmentioning

confidence: 58%

“…Constant et al reported that an increased number of rotations per minute for a shorter period increased the platelet enrichment of the PRP sample without changing leukocyte or erythrocyte concentrations of the PRP when processing cow blood. 17 However, they pierced the buffy coat during aspiration in every sample, which further confounds comparison of the results with this study's results. The methods used in this study only sampled the most proximal and most distal (buffy-coat adjacent) PRP, which limited the ability to comment on the distribution of cellular components at all levels of the PRP.…”

Section: Discussionmentioning

confidence: 70%

The effect of two platelet‐rich plasma aspiration techniques on plasma cellular concentrations using a double syringe gravitational centrifugation system

Jones¹,

Frederick²,

Cross³

2023

Veterinary Surgery

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ObjectiveTo identify which aspiration technique increased plasma platelet concentration and which technique minimized plasma leukocyte and erythrocyte concentrations using a gravitational double‐syringe platelet rich plasma (PRP) system.Study designControlled laboratory study.AnimalsThirty adult dogs.MethodsWhole blood was collected into two autologous conditioned plasma (ACP) syringes and an ethylenediaminetetraacetic acid (EDTA tube) (control samples). The ACP syringes were centrifuged for 5 min at 1500 rpm. The proximal 2 mL of plasma from one ACP syringe was deposited in an EDTA tube (preflash samples). Plasma from the second ACP syringe was withdrawn until the buffy coat was pierced, producing a “flash” of red blood cells, agitated and deposited into an EDTA tube (flash samples). Complete blood counts were performed.ResultsMean plasma platelet concentrations of the control, preflash, and flash samples were 2.4 × 105/dL, 3.3 × 105/dL and 4.1 × 105/dL, respectively. The mean platelet concentration of the flash samples was 7.9 × 104/dL higher than the preflash samples (p = .005). The mean platelet concentration was lower in the control samples than the preflash (p = .002) and flash (p < .0001) samples. The median plasma leukocyte concentration of the preflash samples (0/dL) was lower than in the flash samples (2.4 × 103/dL) (p = .001). The median plasma hematocrit value of the preflash samples (0%) was lower than in the flash samples (1.0%) (p = .002).ConclusionThe flash method is not necessary to produce a PRP sample.Clinical significanceBoth methods produced PRP. However, clinicians should avoid aspirating the buffy coat when processing PRP for therapies where leukocytes and erythrocytes are contraindicated.

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“…PCs are prepared using gravitational centrifugation techniques, standard cell separators, or autologous selective filtration techniques [ 11 ]. Research into the effects of PCs in cattle has increased considerably in recent years; in particular, evidence has shown the positive use of PRP for treating reproductive problems and in vitro production of bovine embryos [ 4 , 5 , 12 , 13 , 14 ]. A recent study has shown the efficacy of PRP in reducing the use of antibiotics in subclinical mastitis [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…While numerous studies have investigated the in vitro effects of PRP [ 13 , 14 , 19 , 21 ], translating these findings into effective in vivo applications remains a subject of ongoing research and debate. The present study seeks to address this gap in the literature by systematically reviewing the existing body of knowledge regarding the in vivo utilisation of bovine PRP (PRP) within the same specimens.…”

Section: Introductionmentioning

confidence: 99%

Clinical Application of Platelet Concentrates in Bovine Practice: A Systematic Review

Caterino,

Della Valle,

Aragosa

et al. 2023

Veterinary Sciences

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Platelet concentrates (PCs) have become widely used in veterinary and human medicine. The PCs consist mainly of supraphysiological concentrations of platelets and, therefore, growth factors (GFs) which are stored within platelet α-granules. Among PCs, Platelet-Rich Plasma (PRP) is characterised by low-density fibrin. Research on the effect of PCs in cattle has surged in recent years; in particular, evidence has shown the positive use of PRP for treating reproductive problems, in vitro production of bovine embryos, sole ulcers and udder diseases. The aim of this report is to critically review, in accordance with the PRISMA guidelines, the available literature reporting clinical application in the bovine practice of PRP. Three bibliographic databases PubMed, Web of Science and Scopus were used for a broad search of “platelet concentrates” OR “PRP” OR “platelet-rich plasma” OR “PRF” OR “platelet-rich fibrin” AND “cows” OR “cattle”. From 1196 papers, only six met the inclusion criteria. Two papers described the use of PRP in mastitis, two papers in uterine dysfunction and two papers in ovarian dysfunction. PRP offered a low-cost, easily obtained therapeutic option and showed positive results for these patients. However, given the different pathologies and definitions involved, further studies are necessary to assess its full clinical potential.

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Single-step production of autologous bovine platelet concentrate for clinical applications in cattle

Cited by 4 publications

References 45 publications

Correlations between Selected Blood Markers of Hematological Profile and Milk Components in Dairy Cows

Correlations between Selected Blood Markers of Hematological Profile and Milk Components in Dairy Cows

The effect of two platelet‐rich plasma aspiration techniques on plasma cellular concentrations using a double syringe gravitational centrifugation system

Clinical Application of Platelet Concentrates in Bovine Practice: A Systematic Review

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