Single-strand conformation polymorphism (SSCP) assays for major histocompatibility complex B genotyping in chickens

Goto, Ronald M.; Afanassieff, Marielle; Ha, Jennifer; Iglesias, Gabriela; Ewald, SJ; Briles, W. E.; Mm, Miller

doi:10.1093/ps/81.12.1832

Cited by 34 publications

(20 citation statements)

References 38 publications

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“…Birds were typed for the BG gene family using Southern hybridizations, as previously described (43). BF and BL types were determined using single strand conformation polymorphism (44). Microsatellite LEI0258 types were revealed by PCR, as previously published (45).…”

Section: Methodsmentioning

confidence: 99%

BG1 has a major role in MHC-linked resistance to malignant lymphoma in the chicken

Goto

Wang

Taylor

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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Pathogen selection is postulated to drive MHC allelic diversity at loci for antigen presentation. However, readily apparent MHC infectious disease associations are rare in most species. The strong link between MHC- B haplotype and the occurrence of virally induced tumors in the chicken provides a means for defining the relationship between pathogen selection and MHC polymorphism. Here, we verified a significant difference in resistance to gallid herpesvirus-2 (GaHV-2)-induced lymphomas (Marek's disease) conferred by two closely-related recombinant MHC- B haplotypes. We mapped the crossover breakpoints that distinguish these haplotypes to the highly polymorphic BG1 locus. BG1 encodes an Ig-superfamily type I transmembrane receptor-like protein that contains an immunoreceptor tyrosine-based inhibition motif (ITIM), which undergoes phosphorylation and is recognized by Src homology 2 domain-containing protein tyrosine phosphatase (SHP-2). The recombinant haplotypes are identical, except for differences within the BG1 3′-untranslated region (3′-UTR). The 3′-UTR of the BG1 allele associated with increased lymphoma contains a 225-bp insert of retroviral origin and showed greater inhibition of luciferase reporter gene translation compared to the other allele. These findings suggest that BG1 could affect the outcome of GaHV-2 infection through modulation of the lymphoid cell responsiveness to infection, a condition that is critical for GaHV-2 replication and in which the MHC- B haplotype has been previously implicated. This work provides a mechanism by which MHC- B region genetics contributes to the incidence of GaHV-2-induced malignant lymphoma in the chicken and invites consideration of the possibility that similar mechanisms might affect the incidence of lymphomas associated with other oncogenic viral infections.

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Section: Methodsmentioning

confidence: 99%

BG1 has a major role in MHC-linked resistance to malignant lymphoma in the chicken

Goto

Wang

Taylor

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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“…To verify the genotypes of BR5 DNA samples, they were analyzed in the context of parental haplotypes (B19 and B21) for BG by Southern hybridization (29), and for MHC I and MHC II by single-strand conformational polymorphism patterns (30). Genotypes for LEI0258 were defined using a LEI0258 primer pair (31).…”

Section: Haplotype Breakpoint Mappingmentioning

confidence: 99%

Extended Gene Map Reveals Tripartite Motif, C-Type Lectin, and Ig Superfamily Type Genes within a Subregion of the Chicken MHC-B Affecting Infectious Disease

Shiina

Briles

Goto

et al. 2007

The Journal of Immunology

116

149

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MHC haplotypes have a remarkable influence on whether tumors form following infection of chickens with oncogenic Marek’s disease herpesvirus. Although resistance to tumor formation has been mapped to a subregion of the chicken MHC-B region, the gene or genes responsible have not been identified. A full gene map of the subregion has been lacking. We have expanded the MHC-B region gene map beyond the 92-kb core previously reported for another haplotype revealing the presence of 46 genes within 242 kb in the Red Jungle Fowl haplotype. Even though MHC-B is structured differently, many of the newly revealed genes are related to loci typical of the MHC in other species. Other MHC-B loci are homologs of genes found within MHC paralogous regions (regions thought to be derived from ancient duplications of a primordial immune defense complex where genes have undergone differential silencing over evolutionary time) on other chromosomes. Still others are similar to genes that define the NK complex in mammals. Many of the newly mapped genes display allelic variability and fall within the MHC-B subregion previously shown to affect the formation of Marek’s disease tumors and hence are candidates for genes conferring resistance.

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“…Several alternative methods have been developed to screen individual genetic variation directly in the domestic chicken, including single-strand conformation polymorphism (Goto et al 2002), automated sequencing (Livant & Ewald 2005) and by using adjacent microsatellites variation ( Fulton et al 2006). All these methods have different advantages, but we used the alternative method of reference strand conformation analysis (RSCA).…”

Section: (A) Study Speciesmentioning

confidence: 99%

Cryptic preference for MHC-dissimilar females in male red junglefowl,Gallus gallus

et al. 2008

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An increasing number of studies test the idea that females increase offspring fitness by biasing fertilization in favour of genetically compatible partners; however, few have investigated or controlled for corresponding preferences in males. Here, we experimentally test whether male red junglefowl, Gallus gallus, prefer genetically compatible females, measured by similarity at the major histocompatibility complex (MHC), a key gene complex in vertebrate immune function. Theory predicts that because some degree of MHC heterozygosity favours viability, individuals should prefer partners that carry MHC alleles different from their own. While male fowl showed no preference when simultaneously presented with an MHC-similar and an MHC-dissimilar female, they showed a 'cryptic' preference, by allocating more sperm to the most MHC-dissimilar of two sequentially presented females. These results provide the first experimental evidence that males might respond to the MHC similarity of a female through differential ejaculate expenditure. By revealing that cryptic male behaviours may bias fertilization success in favour of genetically compatible partners, this study demonstrates the need to experimentally disentangle male and female effects when studying preferences for genetically compatible partners.

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Single-strand conformation polymorphism (SSCP) assays for major histocompatibility complex B genotyping in chickens

Cited by 34 publications

References 38 publications

BG1 has a major role in MHC-linked resistance to malignant lymphoma in the chicken

BG1 has a major role in MHC-linked resistance to malignant lymphoma in the chicken

Extended Gene Map Reveals Tripartite Motif, C-Type Lectin, and Ig Superfamily Type Genes within a Subregion of the Chicken MHC-B Affecting Infectious Disease

Cryptic preference for MHC-dissimilar females in male red junglefowl,Gallus gallus

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