Single symbiotic cell transcriptome sequencing of coral

“…The fresh kidney sample was placed into the GEXSCOPE Tissue Preservation Solution (Singleron Biotechnologies) immediately at 2-8°C (15). After washed by Hanks Balanced Salt Solution (HBSS) for three times, biopsy sample was cut into small pieces (1-2 mm).…”

Section: Kidney Sample Processing and Single-cell Dissociationmentioning

confidence: 99%

“…Subsequently, single cell suspension was loaded onto the microfluidic chip. The single cell RNA-seq libraries were prepared following the manufacturer's protocols (Singleron GEXSCOPE Single Cell RNA-seq Library Kit, Singleron Biotechnologies) (15). The captured single cell RNAseq libraries were sequenced were sequenced by using an Illumina HiSeq X10 instrument and 150 bp paired-end reads.…”

Section: Library Preparation and Preprocessing Of Scrna-seq Datamentioning

confidence: 99%

A Partial Picture of the Single-Cell Transcriptomics of Human IgA Nephropathy

et al. 2021

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The molecular mechanisms underlying renal damage of IgA nephropathy (IgAN) remain incompletely defined. Here, single-cell RNA sequencing (scRNA-seq) was applied to kidney biopsies from IgAN and control subjects to define the transcriptomic landscape at single-cell resolution. We presented a comprehensive scRNA-seq analysis of human renal biopsies from IgAN. We showed for the first time that IgAN mesangial cells displayed increased expression of several novel genes including MALAT1, GADD45B, SOX4, and EDIL3, which were related to cell proliferation and matrix accumulation. The overexpressed genes in tubule cells of IgAN were mainly enriched in inflammatory pathways including TNF signaling, IL-17 signaling, and NOD-like receptor signaling. Furthermore, we compared the results of 4 IgAN patients with the published scRNA-Seq data of healthy kidney tissues of three human donors in order to further validate the findings in our study. The results also verified that the overexpressed genes in tubule cells from IgAN patients were mainly enriched in inflammatory pathways including TNF signaling, IL-17 signaling, and NOD-like receptor signaling. The receptor-ligand crosstalk analysis revealed potential interactions between mesangial cells and other cells in IgAN. IgAN patients with overt proteinuria displayed elevated genes participating in several signaling pathways compared with microproteinuria group. It needs to be mentioned that based on number of mesangial cells and other kidney cells analyzed in this study, the results of our study are preliminary and needs to be confirmed on larger number of cells from larger number of patients and controls in future studies. Therefore, these results offer new insight into pathogenesis and identify new therapeutic targets for IgAN.

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“…The fresh renal biopsy was immediately put into the GEXSCOPE Tissue Preservation Solution (Singleron Biotechnologies) at 2-8°C [18]. The biopsy sample was washed three times with Hanks' Balanced Salt Solution (HBSS), and subsequently cut into small pieces (1-2 mm).…”

Section: Kidney Tissue Processing and Single-cell Dissociationmentioning

confidence: 99%

“…Then the single cell suspension was loaded onto a microfluidic chip. The preparation of scRNA-seq library was performed following the manufacturer's protocol (Singleron GEXSCOPE Single Cell RNA-seq Library Kit, Singleron Biotechnologies) [18].…”

Section: Library Preparation and Scrna-seq Data Preprocessingmentioning

confidence: 99%

Single-cell RNA Sequencing Reveals Transcriptional Signatures and Cell-cell Crosstalk in Patients With Hypertensive Nephropathy

Tang

Lin

Shen

et al. 2021

Preprint

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BackgroundHypertensive nephropathy (HTN) is one of the leading causes of end-stage renal disease, yet the precise mechanisms and cell-specific gene expression changes are still unknown. This study used single-cell RNA sequencing (scRNA-seq) to explore novel molecular mechanisms and gene targets for HTN for the first time. Methods: The gene expression profiles of renal biopsy samples obtained from HTN patients and healthy living donor controls were determined by scRNA-seq technology. Distinct cell clusters, differential gene expression, cell-cell interaction and potential signaling pathways involved in HTN were determined. Results18 distinct cell clusters were identified in kidney from HTN and control subjects. Endothelial cells overexpressed LRG1 , a pleiotropic factor linked to apoptosis and inflammation, providing a potential novel molecular target. HTN endothelium also overexpressed genes linked to cellular adhesion, extracellular matrix accumulation and inflammation. In HTN patients, mesangial cells highly expressed proliferation related signatures ( MGST1 , TMSB10, EPS8 and IER2 ) not detected in renal diseases before. The upregulated genes in tubules of HTN were mainly participating in inflammatory signatures including IFN-γ signature, IL-17 signaling and TLR signaling. Specific gene expression of kidney-resident CD8 + T cells exhibited a proinflammatory, chemotactic and cytotoxic phenotype. Furthermore, receptor-ligand interaction analysis indicated cell-cell crosstalk in kidney contributes to recruitment and infiltration of inflammatory cells into kidneys, and fibrotic process in hypertensive renal injury. ConclusionsIn summary, our data identifies a distinct cell-specific gene expression profile, pathogenic signaling pathways and potential cell-cell communications in the pathogenesis of HTN. These findings will provide a promising novel landscape for mechanisms and treatment of HTN.

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Single symbiotic cell transcriptome sequencing of coral

Cited by 7 publications

References 36 publications

Single-cell RNA-seq analysis identifies unique chondrocyte subsets and reveals involvement of ferroptosis in human intervertebral disc degeneration

Single-cell RNA-seq analysis identifies unique chondrocyte subsets and reveals involvement of ferroptosis in human intervertebral disc degeneration

A Partial Picture of the Single-Cell Transcriptomics of Human IgA Nephropathy

Single-cell RNA Sequencing Reveals Transcriptional Signatures and Cell-cell Crosstalk in Patients With Hypertensive Nephropathy

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