Single-tube four-target lateral flow assay detects human papillomavirus types associated with majority of cervical cancers

Barra, Maria; Chang, Megan; Salcedo, Mila P.; Schmeler, Kathleen; Scheurer, Michael; Maza, Mauricio; Lopez, Leticia; Alfaro, Karla; Richards-Kortum, Rebecca

doi:10.1016/j.ab.2024.115480

Analytical Biochemistry

2024

DOI: 10.1016/j.ab.2024.115480

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Single-tube four-target lateral flow assay detects human papillomavirus types associated with majority of cervical cancers

Maria Barra,

Megan Chang,

Mila P. Salcedo

et al.

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2024

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Cited by 2 publications

References 27 publications

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Isothermal Technologies for HPV Detection: Current Trends and Future Perspectives

Flores-Contreras,

González-González,

Trujillo-Rodríguez

et al. 2024

Pathogens

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The human papillomavirus (HPV) is a non-enveloped DNA virus transmitted through skin-to-skin contact that infects epithelial and mucosal tissue. It has over 200 known genotypes, classified by their pathogenicity as high-risk and low-risk categories. High-risk HPV genotypes are associated with the development of different types of cancers, including cervical cancer, which is a leading cause of mortality in women. In clinical practice and the market, the principal tests used to detect HPV are based on cytology, hybrid detection, and qPCR. However, these methodologies may not be ideal for the required timely diagnosis. Tests have been developed based on isothermal nucleic acid amplification tests (INAATs) as alternatives. These tests offer multiple advantages over the qPCR, such as not requiring specialized laboratories, highly trained personnel, or expensive equipment like thermocyclers. This review analyzes the different INAATs applied for the detection of HPV, considering the specific characteristics of each test, including the HPV genotypes, gene target, the limit of detection (LOD), detection methods, and detection time. Additionally, we discuss the tests available on the market that are approved by the Food and Drug Administration (FDA). Finally, we address the challenges and potential solutions for the large-scale implementation of INAATs, particularly in rural or underserved areas.

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Isothermal Technologies for HPV Detection: Current Trends and Future Perspectives

Flores-Contreras,

González-González,

Trujillo-Rodríguez

et al. 2024

Pathogens

View full text Add to dashboard Cite

show abstract

Isothermal Nucleic Acid Amplification for Point-of-Care Primary Cervical Cancer Screening

Lamsisi,

Benlghazi,

Kouach

et al. 2024

Viruses

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Human Papillomavirus (HPV) infection is a significant global health concern linked to various cancers, particularly cervical cancer. Timely and accurate detection of HPV is crucial for effective management and prevention strategies. Traditional laboratory-based HPV testing methods often suffer from limitations such as long turnaround times, restricted accessibility, and the need for trained personnel, especially in resource-limited settings. Consequently, there is a growing demand for point-of-care (POC) HPV testing solutions that offer rapid, easy-to-use, and convenient screening at the primary care level. This review provides a comprehensive overview of recent advancements and emerging technologies utilized in POC HPV testing using isothermal amplification methods, in addition to evaluating their diagnostic performance, sensitivity, specificity, and clinical utility compared to conventional laboratory-based assays, particularly in low-resource settings, where access to centralized laboratory facilities is limited. We provide insights into the potential of isothermal nucleic acid amplification to revolutionize cervical cancer screening and prevention efforts worldwide, with emphasis on the need for continued research, innovation, and collaboration to optimize the performance, accessibility, and affordability of POC HPV testing solutions, ultimately contributing to the worldwide effort towards the elimination of this disease.

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Single-tube four-target lateral flow assay detects human papillomavirus types associated with majority of cervical cancers

Cited by 2 publications

References 27 publications

Isothermal Technologies for HPV Detection: Current Trends and Future Perspectives

Isothermal Technologies for HPV Detection: Current Trends and Future Perspectives

Isothermal Nucleic Acid Amplification for Point-of-Care Primary Cervical Cancer Screening

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