Single‐Use Sensor Platforms Based on Carbon Nanotubes for Electrochemical Detection of DNA Hybridization Related to <i>Microcystis</i> spp.

Erdem, Arzum; Karadeniz, Hakan; Canavar, Pembe Ece; Çongur, Gülşah

doi:10.1002/elan.201100369

Cited by 30 publications

(11 citation statements)

References 36 publications

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“…On the other hand, the average R ct values were calculated as 1278.00±535.99 Ohm (RSD %, 41.94 %; n=2) and 707.00±98.99 Ohm (RSD %, 14 %; n=2) after following 50 μg/mL fsDNA immobilization onto the surface of unmodified PGE and CHIT‐PGE, respectively. This increase at R ct value could be a result of the negative charges coming from phosphate backbone of double stranded DNA preventing the redox couple, [Fe(CN) 6 ] 3−/4− from reaching the electrode surface .…”

Section: Resultsmentioning

confidence: 99%

Chitosan‐carbon Nanofiber Modified Single‐use Graphite Electrodes Developed for Electrochemical Detection of DNA Hybridization Related to Hepatitis B Virus

Ekşin

Erdem

2016

Electroanalysis

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1IntroductionHepatitis Bv irus (HBV) infection is am ajor worldwide public health problem with acute and chronic clinical consequences.H BV is one of the causative agents of viral hepatitis,t hat may lead to chronic hepatitis, cirrhosis, and primaryl iver cancer. Accordingt ot he WHO,m ore than 240 million people have chronic (long-term) liver infections and moret han 780 000 people die every year due to the consequences of Hepatitis B[ 1].T he goal of antiviral therapyofchronic hepatitis Bisthe sustained suppression or loss of detectable HBV DNAi ns erum, which is detected by nucleic acid amplification and hybridization techniques [2].T hereh as been ag rowing interest for fast, reliable and ultrasensitive biosensors for detection of trace amounts of DNA [ 3].E lectrochemical detection systemsa re amendablet om iniaturization using well established microfabrication techniques.Among the availablet echniques, electrochemical based biosensors have been one of the mosts uccessful ones that are offering less expensive,f aster,s impler, portable,a ccurate,f lexible for multiplexing and label free detectiono f aw ide range of proteins,n ucleic acids,e nzymes and other biomolecules.I na ddition, they are promising candidate for advanced biosensor platform [4,5].A fter the implementation of single and multi-walled carbonn anotubes (CNTs)[ 6],c arbonn anofibers (CNFs) [7,8],g raphene [9],g old nanowires,n anostructures [10] and other inorganic wires [11] to sensort echnologyh ave tremendously improved the sensitivity and detection limits for proteins and nucleic acids etc. [12].T he constructedb iosensors can be employed with conventional electrochemical techniques;s uch as cyclic voltammetry( CV) [13],d ifferential pulse voltammetry (DPV)[ 14],a nd electrochemicali mpendence spectroscopy (EIS) [14-17].Among carbon nanomaterials,C NFs have excellent conductive and structural properties.C NFs with ah igh aspect ratio have advantages in both electricalc onductivity and providing as pecific surface area [18].C NFs are extremely attractive for development of novel sensor surfaces,a st hey provide such properties as high surface area, non-toxicity,a cceptable biocompatibility [19][20][21][22]. In addition,C NF is produced 100 timesl ower in cost than single walled carbon nanotubes [ 23].Chitosan (CHIT), ap olysaccharide,i st he N-deacetylated derivative of chitin,w hich has ah igh content of hydroxyl and amino groups alongits chains [24].I thas been widely used for the development of biosensors due to its properties;m echanical strength,p ore size,b iocompatibility,e xcellent membrane-forming ability,i on-transport and ion-exchange [25,26].Abstract:C hitosan (CHIT) and carbon nanofiber( CNF) modified pencil graphite electrodes (PGEs) were developed for the first time in the presents tudy for enhanced monitoring of DNAh ybridization.C HIT-CNF modified PGE, CHIT modified PGE and unmodified PGE were firstly characterized by scanninge lectronem icroscopy (SEM),a nd the electrochemical behaviour of each electrode was investigated b...

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Section: Resultsmentioning

confidence: 99%

Chitosan‐carbon Nanofiber Modified Single‐use Graphite Electrodes Developed for Electrochemical Detection of DNA Hybridization Related to Hepatitis B Virus

Ekşin

Erdem

2016

Electroanalysis

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“…Nucleic acid analysis using biosensing strategies has been an attracting topic in many fields including gene analysis, clinical disease diagnosis, biological, environmental, pharmaceutical and forensic applications [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 ]. Several detection techniques such as optic [ 9 , 10 ], acoustic [ 11 ], quartz crystal microbalance [ 12 ], and different electrochemical techniques [ 1 , 2 , 4 , 5 , 6 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ] have been used for nucleic acid analysis.…”

Section: Introductionmentioning

confidence: 99%

“…Effective immobilization of oligonucleotides onto the surface of analysis platform has been one of the key features for the successful design of biosensor platforms [ 1 , 2 , 4 , 5 , 6 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. Ionic liquids (ILs) are known as low-melting organic salts and mostly liquid at room temperature.…”

Section: Introductionmentioning

confidence: 99%

“…The microcystins (MYC), which are a family of cyclic polypeptides produced by different species of cyanobacteria, are among the most frequently detected in fresh waters, and produce potent toxic effects. Consumption of contaminated waters and food (agricultural products, fish, prawns, and mollusks), can cause human diseases such as gastroenteritis and related diseases, allergic and irritation reactions, liver cancer and colorectal cancers in consequence of exposure to low MYC concentrations through dermal exposure and inhalation [ 14 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

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Development of Ionic Liquid Modified Disposable Graphite Electrodes for Label-Free Electrochemical Detection of DNA Hybridization Related to Microcystis spp.

Sengiz

Çongur

Erdem

2015

Sensors

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In this present study, ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate (IL)) modified pencil graphite electrode (IL-PGEs) was developed for electrochemical monitoring of DNA hybridization related to Microcystis spp. (MYC). The characterization of IL-PGEs was performed using microscopic and electrochemical techniques. DNA hybridization related to MYC was then explored at the surface of IL-PGEs using differential pulse voltammetry (DPV) technique. After the experimental parameters were optimized, the sequence-selective DNA hybridization related to MYC was performed in the case of hybridization between MYC probe and its complementary DNA target, noncomplementary (NC) or mismatched DNA sequence (MM), or and in the presence of mixture of DNA target: NC (1:1) and DNA target: MM (1:1).

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“…19,20 Au NPs can accelerate the electron transfer between the electrode surface and modiers, and further amplify the electrical response signals. 6,[40][41][42] Therefore, our biosensor is an economic, sensitive sensor for Microcystis spp. [23][24][25][26][27] Metal membranes on electrode surfaces, as a continuous lm, particles, colloids, monolayer, or electrostatically bound particles, should signicantly amplify electrochemical signals during biomolecular recognition, and this has greatly improved the sensitivity of DNA hybridization detection.…”

Section: Introductionmentioning

confidence: 99%

A sensitive electrochemical DNA biosensor for Microcystis spp. sequence detection based on an Ag@Au NP composite film

et al. 2015

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An electrochemical DNA biosensor was developed for detecting a gene sequence related to blooming genera of Cyanobacteria, Microcystis spp. In this sensor, gold nanoparticles (Au NPs) were electrodeposited on a gold electrode (GE) surface to enhance the amount of DNA immobilization. Silver was anchored on Au NPs to increase the electrochemical signal of hybridization because metallic films deposited as a continuous layer on a solid electrode can greatly enhance the rate of electron transfer. In the differential peak voltammograms, the peak current of the methylene blue (MB) hybridization indicator was about 10 times that of direct detection. Therefore, lower detection limits and a wider linear range for the biosensor were obtained. Based on silver enhancement, the change in the reduction peak current before and after hybridization (Di p ) of MB was linearly related to the concentration of the target DNA sequence in the range of 3.0 Â 10 À12 to 1.2 Â 10 À10 mol L À1 with a detection limit of 1.6 Â 10 À12 mol L À1 . In addition, the biosensor had good selectivity, and the base mismatch sequences from specific species could be distinguished.

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Single‐Use Sensor Platforms Based on Carbon Nanotubes for Electrochemical Detection of DNA Hybridization Related to Microcystis spp.

Cited by 30 publications

References 36 publications

Chitosan‐carbon Nanofiber Modified Single‐use Graphite Electrodes Developed for Electrochemical Detection of DNA Hybridization Related to Hepatitis B Virus

Chitosan‐carbon Nanofiber Modified Single‐use Graphite Electrodes Developed for Electrochemical Detection of DNA Hybridization Related to Hepatitis B Virus

Development of Ionic Liquid Modified Disposable Graphite Electrodes for Label-Free Electrochemical Detection of DNA Hybridization Related to Microcystis spp.

A sensitive electrochemical DNA biosensor for Microcystis spp. sequence detection based on an Ag@Au NP composite film

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