Single-Walled Carbon Nanotube (SWCNT)-induced interstitial fibrosis in the lungs of rats is associated with increased levels of PDGF mRNA and the formation of unique intercellular carbon structures that bridge alveolar macrophages In Situ

Mangum, James B.; Turpin, Elizabeth A.; Antao-Menezes, Aurita; Cesta, Mark F.; Bermudez, Edilberto; Bonner, James C.

doi:10.1186/1743-8977-3-15

Cited by 118 publications

(57 citation statements)

References 22 publications

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“…Macrophages undergoing “frustrated phagocytosis” and formation of FBGC in response to MWCNT have been reported earlier after intraperitoneal injection using the same material as in our study [10]. Mangum et al [25] found carbon bridges between macrophages after a single oropharyngeal aspiration of SWCNT in rats. Giant cells arise from a fusion of macrophages in response to large foreign material [26].…”

Section: Discussionsupporting

confidence: 84%

Inhalation of rod-like carbon nanotubes causes unconventional allergic airway inflammation

et al. 2014

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BackgroundCarbon nanotubes (CNT) represent a great promise for technological and industrial development but serious concerns on their health effects have also emerged. Rod-shaped CNT are, in fact, able to induce asbestos-like pathogenicity in mice including granuloma formation in abdominal cavity and sub-pleural fibrosis. Exposure to CNT, especially in the occupational context, happens mainly by inhalation. However, little is known about the possible effects of CNT on pulmonary allergic diseases, such as asthma.MethodsWe exposed mice by inhalation to two types of multi-walled CNT, rigid rod-like and flexible tangled CNT, for four hours a day once or on four consecutive days. Early events were monitored immediately and 24 hours after the single inhalation exposure and the four day exposure mimicked an occupational work week. Mast cell deficient mice were used to evaluate the role of mast cells in the occurring inflammation.ResultsHere we show that even a short-term inhalation of the rod-like CNT induces novel innate immunity-mediated allergic-like airway inflammation in healthy mice. Marked eosinophilia was accompanied by mucus hypersecretion, AHR and the expression of Th2-type cytokines. Exploration of the early events by transcriptomics analysis reveals that a single 4-h exposure to rod-shaped CNT, but not to tangled CNT, causes a radical up-regulation of genes involved in innate immunity and cytokine/chemokine pathways. Mast cells were found to partially regulate the inflammation caused by rod-like CNT, but also alveaolar macrophages play an important role in the early stages.ConclusionsThese observations emphasize the diverse abilities of CNT to impact the immune system, and they should be taken into account for hazard assessment.Electronic supplementary materialThe online version of this article (doi:10.1186/s12989-014-0048-2) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 84%

Inhalation of rod-like carbon nanotubes causes unconventional allergic airway inflammation

et al. 2014

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“…These results demonstrate that the inhalation uptake of aerosolized SWNTs produces stronger, acute, and chronic inflammatory reactions in the lungs of mice compared with PA with aggregated SWNTs. In fact, progressive and dose-dependent oxidative damages and interstitial fibrosis were among the major recurrent findings that were observed in most of the pulmonary toxicity studies for SWNTs (Mangum et al 2006;Park et al 2011;Shvedova et al 2005Shvedova et al , 2007Tyurina et al 2011).…”

Section: Pulmonary Toxicitymentioning

confidence: 97%

Toxicity of single-walled carbon nanotubes

et al. 2014

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Carbon nanotubes (CNTs) are an important class of nanomaterials, which have numerous novel properties that make them useful in technology and industry. Generally, there are two types of CNTs: single-walled nanotubes (SWNTs) and multi-walled nanotubes. SWNTs, in particular, possess unique electrical, mechanical, and thermal properties, allowing for a wide range of applications in various fields, including the electronic, computer, aerospace, and biomedical industries. However, the use of SWNTs has come under scrutiny, not only due to their peculiar nanotoxicological profile, but also due to the forecasted increase in SWNT production in the near future. As such, the risk of human exposure is likely to be increased substantially. Yet, our understanding of the toxicological risk of SWNTs in human biology remains limited. This review seeks to examine representative data on the nanotoxicity of SWNTs by first considering how SWNTs are absorbed, distributed, accumulated and excreted in a biological system, and how SWNTs induce organ-specific toxicity in the body. The contradictory findings of numerous studies with regards to the potential hazards of SWNT exposure are discussed in this review. The possible mechanisms and molecular pathways associated with SWNT nanotoxicity in target organs and specific cell types are presented. We hope that this review will stimulate further research into the fundamental aspects of CNTs, especially the biological interactions which arise due to the unique intrinsic characteristics of CNTs.

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“…Exposure of CNTs exerts negative effects on the normal physiological functions of respiratory system, cardiovascular system, and immune system, inducing inflammation, granulomas and sub-pleural fibrosis 12–14 , progression of atherosclerosis 15, 16 , and allergic immune responses 17 . The highly phagocytic macrophages represent the first line of defense against pathogens and particles and are one of the major cell types involved in uptake and clearance of these particles 18 . They are well recognized as a major source of pro-inflammatory and fibrogenic mediators, including cytokines, growth factors, and reactive oxygen species (ROS) 19 .…”

Section: Introductionmentioning

confidence: 99%

Length effects on the dynamic process of cellular uptake and exocytosis of single-walled carbon nanotubes in murine macrophage cells

Cui

Wan

Yang

et al. 2017

Sci Rep

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Cellular uptake and exocytosis of SWCNTs are fundamental processes determining their intracellular concentration and effects. Despite the great potential of acid-oxidized SWCNTs in biomedical field, understanding of the influencing factors on these processes needs to be deepened. Here, we quantitatively investigated uptake and exocytosis of SWCNTs in three lengths-630 (±171) nm (L-SWCNTs), 390 (±50) nm (M-SWCNTs), and 195 (±63) nm (S-MWCNTs) in macrophages. The results showed that the cellular accumulation of SWCNTs was a length-independent process and non-monotonic in time, with the most SWCNTs (3950 fg/cell) accumulated at 8 h and then intracellular SWCNTs dropped obviously with time. The uptake rate of SWCNTs decreased with increasing concentration, suggesting that intracellular SWCNTs accumulation is a saturable process. After refreshing culture medium, we found increasing SWCNTs in supernatant and decreasing intracellular SWCNTs over time, confirming the exocytosis occurred. Selective inhibition of endocytosis pathways showed that the internalization of SWCNTs involves several pathways, in the order of macropinocytosis> caveolae-mediated endocytosis> clathrin-dependent endocytosis. Intriguingly, clathrin-mediated endocytosis is relatively important for internalizing shorter SWCNTs. The dynamic processes of SWCNTs uptake and exocytosis and the mechanisms revealed by this study may render a better understanding on SWCNT toxicity and facilitate the design of CNT products with mitigated toxicity and desired functions.

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Single-Walled Carbon Nanotube (SWCNT)-induced interstitial fibrosis in the lungs of rats is associated with increased levels of PDGF mRNA and the formation of unique intercellular carbon structures that bridge alveolar macrophages In Situ

Cited by 118 publications

References 22 publications

Inhalation of rod-like carbon nanotubes causes unconventional allergic airway inflammation

Inhalation of rod-like carbon nanotubes causes unconventional allergic airway inflammation

Toxicity of single-walled carbon nanotubes

Length effects on the dynamic process of cellular uptake and exocytosis of single-walled carbon nanotubes in murine macrophage cells

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