Single-walled carbon nanotubes (SWCNTs) inhibit heat shock protein 90 (HSP90) signaling in human lung fibroblasts and keratinocytes

Ong, Li-Chu; Tan, Yin; Tan, Boon Shing; Chung, Felicia Fei‐Lei; Cheong, Soon-Keng; Leong, Chee‐Onn

doi:10.1016/j.taap.2017.06.024

Cited by 13 publications

(4 citation statements)

References 67 publications

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“…Because the SG-CNTs became structurally damaged (Figure 1D), and because carboxylic groups are induced after partial degradation of CNTs by ROS-mediated oxidation,33 the remaining residues of CNTs would be more hydrophilic and more biocompatible, which might explain the decrease in ROS levels as SG-CNT degradation decreased. In addition, recent studies have shown that SWNTs could inhibit the heat shock protein 90 (HSP90)34 and that inhibition of HSP90 could reduce the NADPH oxidases 35. This might be another reason for the regulation of ROS levels following the CNT exposure.…”

Section: Discussionmentioning

confidence: 99%

<p>Time-dependent degradation of carbon nanotubes correlates with decreased reactive oxygen species generation in macrophages</p>

Yang¹,

Zhang²,

Nakajima³

et al. 2019

IJN

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Introduction and objective: With the increase in carbon nanotube-based products on the commercial market, public concern regarding the possible toxicity of these nanomaterials has attracted much attention. Although previous studies found no obvious toxicity related to carbon nanotubes (CNTs), their safety has not been established because long-term evaluation is still needed. In vitro assays are used to understand the toxicity of nanomaterials. However, the data published so far were generated in short-term assays in which cells are continuously exposed to CNTs. Therefore, the objective of this study is to quantitatively assess the relative long-term cytotoxicity and degradation of CNTs after uptake by macrophages. Methods: We used macrophage cell line of RAW 264.7 and primary rat Kupffer cells to investigate macrophage uptake of CNTs as well as their quantity changes up to a relatively late time point after uptake (7 days) by measuring optical absorbance in the near infrared region and Raman spectra of CNTs in the cell lysates. The time-dependent cytotoxicity was evaluated by measuring reactive oxygen species (ROS), glutathione, cell viability, and caspase 3/7 activity in 1–7 days. Results: CNTs were degraded by approximately 25–30% within first 4 days after uptake; however, and no additional degradation occurred for the remainder of the 7-day test period. Generation of ROS by macrophages decreased as CNT degradation occurred, returning to control levels by Day 7. In the meantime, the glutathione level gradually recovered over time. There were no changes in cell viability or caspase 3/7 activation during CNT degradation. Conclusion: These results confirm that degradation of CNTs by macrophages is associated with ROS generation. The data also suggest that CNT cytotoxicity decreases as they are degraded.

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Section: Discussionmentioning

confidence: 99%

<p>Time-dependent degradation of carbon nanotubes correlates with decreased reactive oxygen species generation in macrophages</p>

Yang¹,

Zhang²,

Nakajima³

et al. 2019

IJN

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“…Besides their ability to upregulate NF-κB in a concentration-dependent mode, they stimulate its binding to DNA, initiating the transcription for mediators involved in inflammation and cell death [88]. In addition, the downstream pathways associated with SWCNTs effects on HaCaT cells seemed to be dependent upon inhibition of the activity of heat shock protein 90 (HSP90), a molecular chaperone facilitating proteins folding and assembly, assuring the conformational stability and functionality of several HSP90 client proteins, including AKT, CDK4 and BCL-2, involved in a series of pathways modulating cellular exo/endocytosis, signal transduction, chromatin remodeling, cell growth and proliferation [89].…”

Section: Mechanisms Of Toxicitymentioning

confidence: 99%

“…In vivo and in vitro toxicokinetic data and adverse effects of carbon nanotubes at the skin level (N/A: data not available). [ 85,[87][88][89]92] a N-SWCNTs (Nikkiso-SWCNTs); SG-SWCNTs (super growth SWCNTs); N-MWCNTs (Nikkiso-MWCNTs); MWNT-7 (Mitsui product of MWCNTs).…”

Section: Tablementioning

confidence: 99%

CARBON-BASED nanomaterials and SKIN: An overview

Colletta

Pelin

Sosa

et al. 2022

Carbon

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“…For instance, ZnO NPs are observed to be harmful to both in vitro (MRC-5 cell line) and in vivo (Drosophila) models, resulting in a significant increase in oxidative stress, decreased cellular viability genotoxicity and impairment of egg-to-adult viability (Ng et al, 2017). Also, SWCNT treated MRC-5 cells has shown a reduction in cell viability, significantly increased apoptosis and necrosis (Ong et al, 2017). In this context, the primary question arises whether this toxicological behaviour is based on the type of NPs or because of common characteristics shared by diverse NPs.…”

Section: Introductionmentioning

confidence: 99%

ZnO nanoparticles and SWCNT induced general stress response pathway in HepG2 cells at non-cytotoxic doses revealed by RNA sequencing

Mittal

Ali

Kaul

2022

Preprint

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Nanoparticles (NPs) are important in a variety of sectors, including disease diagnostics, medicine, nutrition, and many other industries. The risk of human exposure demands an early evaluation of both the basic dynamics of NPs' interaction with biological systems and their potential consequences. Deciphering these occurrences will provide critical information regarding the health hazards and safety advantages associated with next-generation nanoformulations in clinical practice. We examined the HepG2 cell line in a systematic manner to determine the cellular response to single-walled carbon nanotubes (SWCNTs) and zinc oxide (ZnO) NPs. With the use of high-throughput transcriptomic methods, we found that both NPs induce comparable dysregulation of the endocytic and proteasomal complex genes in liver hepatocellular carcinoma cells, at levels (> 80 percent cell viability) that do not cause over-toxicity at early incubation period (6 h). SWCNT and ZnO NPs were shown to enter cells through clathrin-mediated pathways, affecting cytoskeleton gene expression, DNA damage and repair, protein ubiquitination, and cell transcriptional machinery. Our findings indicate that early response strategies activate stress-related mechanisms.

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Single-walled carbon nanotubes (SWCNTs) inhibit heat shock protein 90 (HSP90) signaling in human lung fibroblasts and keratinocytes

Cited by 13 publications

References 67 publications

<p>Time-dependent degradation of carbon nanotubes correlates with decreased reactive oxygen species generation in macrophages</p>

<p>Time-dependent degradation of carbon nanotubes correlates with decreased reactive oxygen species generation in macrophages</p>

CARBON-BASED nanomaterials and SKIN: An overview

ZnO nanoparticles and SWCNT induced general stress response pathway in HepG2 cells at non-cytotoxic doses revealed by RNA sequencing

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