Singlet-Contrast Magnetic Resonance Imaging

Eills, James; Cavallari, Eleonora; Kircher, Raphael; G, Di Matteo; Carrera, Carla; Dagys, Laurynas; Mh, Levitt; Ivanov, Konstantin L.; Aime, Silvio; Reineri, Francesca; Münnemann, Kerstin; Budker, Dmitry; Buntkowsky, Gerd; Knecht, Stephan

doi:10.26434/chemrxiv.12863639.v1

Cited by 4 publications

(4 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This allows observation of only the hyperpolarized component, thus making cis‐ 15 N 2 ‐ABZ an interesting molecule, that can be used in singlet‐contrast MRI experiments. [31] …”

Section: Discussionmentioning

confidence: 99%

Hyperpolarization of cis‐¹⁵N₂‐Azobenzene by Parahydrogen at Ultralow Magnetic Fields**

et al. 2021

View full text Add to dashboard Cite

The development of nuclear spins hyperpolarization, and the search for molecules that can be efficiently hyperpolarized is an active area in nuclear magnetic resonance. In this work we present a detailed study of SABRE SHEATH (signal amplification by reversible exchange in shield enabled alignment transfer to heteronuclei) experiments on 15 N 2 -azobenzene. In SABRE SHEATH experiments the nuclear spins of the target are hyperpolarized through transfer of spin polarization from parahydrogen at ultralow fields during a reversible chemical process. Azobenzene exists in two isomers, trans and cis. We show that all nuclear spins in cis-azobenzene can be efficiently hyperpolarized by SABRE at suitable magnetic fields. Enhancement factors (relative to 9.4 T) reach up to 3000 for 15 N spins and up to 30 for the 1 H spins. We compare two approaches to observe either hyperpolarized magnetization of 15 N/ 1 H spins, or hyperpolarized singlet order of the 15 N spin pair. The results presented here will be useful for further experiments in which hyperpolarized cis-15 N 2 -azobenzene is switched by light to trans-15 N 2 -azobenzene for storing the produced hyperpolarization in the long-lived spin state of the 15 N pair of trans-15 N 2 -azobenzene.[ + ] Deceased

show abstract

“…This allows observation of only the hyperpolarized component, thus making cis‐ 15 N 2 ‐ABZ an interesting molecule, that can be used in singlet‐contrast MRI experiments. [31] …”

Section: Discussionmentioning

confidence: 99%

Hyperpolarization of cis‐¹⁵N₂‐Azobenzene by Parahydrogen at Ultralow Magnetic Fields**

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Commonly the inequivalence is caused by a chemical shift difference between the two protons that is greater than their mutual J-coupling. However, if the protons are close to chemical or magnetic equivalence, the non-magnetic singlet state remains close to an eigenstate, and either electromagnetic pulses [188] or further chemical reactions [189,190] are needed to release the singlet order to generate observable hyperpolarized signals.…”

Section: Hydrogenative Phipmentioning

confidence: 99%

Synergies between Hyperpolarized NMR and Microfluidics: A Review

Eills¹,

Hale²,

Utz³

2021

Preprint

Self Cite

View full text Add to dashboard Cite

Hyperpolarized nuclear magnetic resonance and lab-on-a-chip microfluidics are two dynamic, but until recently quite distinct, fields of research. Recent developments in both areas increased their synergistic overlap. By microfluidic integration, many complex experimental steps can be brought together onto a single platform. Microfluidic devices are therefore increasingly finding applications in medical diagnostics, forensic analysis, and biomedical research. In particular, they provide novel and powerful ways to culture cells, cell aggregates, and even functional models of entire organs. Nuclear magnetic resonance is a non-invasive, high-resolution spectroscopic technique which allows real-time process monitoring with chemical specificity. It is ideally suited for observing metabolic and other biological and chemical processes in microfluidic systems. However, its intrinsically low sensitivity has limited its application. Recent advances in nuclear hyperpolarization techniques may change this: under special circumstances, it is possible to enhance NMR signals by up to 5 orders of magnitude, which dramatically extends the utility of NMR in the context of microfluidic systems. At the same time, hyperpolarization requires complex chemical and/or physical manipulations, which in turn may benefit from microfluidic implementation. In fact, many hyperpolarization methodologies rely on processes that are more efficient at the micro-scale, such as molecular diffusion, penetration electromagnetic radiation into the sample, or restricted molecular mobility on a surface. In this review we examine the confluence between the fields of hyperpolarization-enhanced NMR and microfluidics, and assess how these areas of research have mutually benefited one another, and will continue to do so.

show abstract

“…Recently, Ellis et al presented how fumarate could be hyperpolarized via chemical reaction of acetylene dicarboxylate with para-enriched hydrogen gas leading to para-fumarate. Then, the proton singlet order in fumarate could be released as antiphase NMR signals by enzymatic conversion to malate in D2O [81].…”

Section: Long-lived Statesmentioning

confidence: 99%

Hyperpolarization via dissolution dynamic nuclear polarization: new technological and methodological advances

Pinon

Capozzi

Ardenkjær-Larsen

2020

Magn Reson Mater Phy

View full text Add to dashboard Cite

Dissolution-DNP is a method to boost liquid-state NMR sensitivity by several orders of magnitude. The technique consists in hyperpolarizing samples by solid-state dynamic nuclear polarization at low temperature and moderate magnetic field, followed by an instantaneous melting and dilution of the sample happening inside the polarizer. Although the technique is well established and the outstanding signal enhancement paved the way towards many applications precluded to conventional NMR, the race to develop new methods allowing higher throughput, faster and higher polarization and longer exploitation of the signal is still vivid. In this work, we review the most recent advances on dissolution-DNP methods trying to overcome the original technique's shortcomings. The review describes some of the new approaches in the field, firstly, in terms of sample formulation and properties, secondly, in terms of instrumentation.

show abstract

Singlet-Contrast Magnetic Resonance Imaging

Cited by 4 publications

References 59 publications

Hyperpolarization of cis‐¹⁵N₂‐Azobenzene by Parahydrogen at Ultralow Magnetic Fields**

Hyperpolarization of cis‐¹⁵N₂‐Azobenzene by Parahydrogen at Ultralow Magnetic Fields**

Synergies between Hyperpolarized NMR and Microfluidics: A Review

Hyperpolarization via dissolution dynamic nuclear polarization: new technological and methodological advances

Contact Info

Product

Resources

About

Singlet-Contrast Magnetic Resonance Imaging

Cited by 4 publications

References 59 publications

Hyperpolarization of cis‐15N2‐Azobenzene by Parahydrogen at Ultralow Magnetic Fields**

Hyperpolarization of cis‐15N2‐Azobenzene by Parahydrogen at Ultralow Magnetic Fields**

Synergies between Hyperpolarized NMR and Microfluidics: A Review

Hyperpolarization via dissolution dynamic nuclear polarization: new technological and methodological advances

Contact Info

Product

Resources

About

Hyperpolarization of cis‐¹⁵N₂‐Azobenzene by Parahydrogen at Ultralow Magnetic Fields**

Hyperpolarization of cis‐¹⁵N₂‐Azobenzene by Parahydrogen at Ultralow Magnetic Fields**