Singlet oxygen and plants

Knox, John; Dodge, A. D.

doi:10.1016/s0031-9422(00)83147-7

Cited by 313 publications

(182 citation statements)

References 142 publications

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“…To our knowledge, artemisinin responded to lead and salt-induced oxidative stress by increasing or decreasing according to the doses of the stressors in the soil (Qureshi et al, 2005) or planting in soil with high salinity (Qian et al, 2007); increased in response to a frost (Wallaart et al, 2000); and significantly increased in response to a mild potassium deficiency (Ferreira, 2007). These authors attributed artemisinin accumulation to the generation of active oxygen species in the plant in response to stress, based on the report that high levels of peroxide can be formed in plants under stress triggered by limited water, high UV-B levels, chilling conditions or tissue damage caused by pathogens or frost (Knox and Dodge, 1985).…”

Section: Discussionmentioning

confidence: 99%

Water deficit effect on the accumulation of biomass and artemisinin in annual wormwood(Artemisia annua L., Asteraceae)

Marchese

Ferreira²,

Rehder

et al. 2010

Braz. J. Plant Physiol.

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Despite the importance of Artemisia annua L. as the only source of the anti-parasitic drug artemisinin, little is known on the effects of biotic and abiotic stress on artemisinin accumulation. Water deficit is the most limiting factor on plant growth, however it can trigger secondary metabolite accumulation, depending on the plant growth stage and intensity. A. annua cultivated in growth chambers was submitted to five water deficit treatments (watered, 14, 38, 62 e 86 hours without irrigation). Water deficits of 38 and 62 hours (Ψw = -1.39 and -2.51 MPa, respectively) increased leaf artemisinin content, but only 38 hours led to a significant increase in both leaf and plant artemisinin (29%), with no detriment to plant biomass production. The other treatments had no effect on, or decreased artemisinin accumulation. A. annua plants tolerated well water deficit treatments, including the most severe water deficit applied (Ψw -3.97 MPa or 86 hs without irrigation) and recovered their turgor pressure after rehydration. These results suggest that moderate water deficit prior to harvesting the crop may not only reduce time and costs in drying the crop, but can also induce artemisinin accumulation, both of which increase crop profit margins. Results also suggest that artemisinin could be part of A. annua chemical system of defense against water deficit.Key words: Malaria; secondary metabolism; terpene; drought; water stress. resuMoA despeito da importância da Artemisia annua L. como a única fonte da droga anti-parasitária artemisinina, muito pouco é sabido sobre o papel dessa molécula em plantas sob estresses biótico e abiótico. A deficiência hídrica é o principal fator limitante no crescimento vegetal, mas pode induzir a acumulação de metabólitos secundários, dependendo da intensidade e fase de desenvolvimento da planta. Plantas de A. annua cultivadas em câmaras de crescimento foram submetidas a cinco tratamentos (plantas irrigadas e com 14, 38, 62 e 86 horas sem irrigação). Deficiências hídricas de 38 e 62 horas (Ψw = -1.39 e -2.51 MPa, respectivamente) aumentaram o conteúdo foliar de artemisinina, mas somente a deficiência hídrica de 38 horas induziu um Braz. J. Plant Physiol., 22(1): 1-9, 2010 2 J. A. MARCHESE et al. aumento significativo da molécula em folhas (29%) e planta inteira, sem afetar o acúmulo de biomassa. Os outros tratamentos não afetaram significativamente o conteúdo de artemisinina. As plantas de A. annua apresentaram uma boa tolerância a deficiência hídrica, incluindo o tratamento mais severo (Ψw -3.97 MPa ou 86 h sem irrigação) e recuperaram a pressão de turgor após a reidratação. Estes resultados sugerem que uma deficiência hídrica moderada imediatamente antes da colheita de A. annua pode não somente reduzir o tempo e os custos de secagem da cultura, mas também induzir ao acúmulo de artemisinina, condições que, conjuntamente, são benéficas para o cultivo commercial da espécie. Finalmente, estes resultados sugerem que a artemisinina pode ser parte do sistema químico de defesa da A. annua c...

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Section: Discussionmentioning

confidence: 99%

Water deficit effect on the accumulation of biomass and artemisinin in annual wormwood(Artemisia annua L., Asteraceae)

Marchese

Ferreira²,

Rehder

et al. 2010

Braz. J. Plant Physiol.

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“…Furthermore, species with high levels of foliar anthocyanin seem to be common in environments characterized by low soil moisture (Spyropoulos and Mavormmatis, 1978), and are more tolerant of drought conditions (Paine et al, 1992). Carotenoids are responsible for quenching of singlet oxygen (Knox and Dodge, 1985) hence their comparative levels in a variety may determine its relative tolerance. Salt stress is a complex phenomenon that involves morphological and developmental changes.…”

Section: Analysesmentioning

confidence: 99%

Salt-Induced Changes in Antioxidative Enzyme Activities in Shoot Tissues of Two Atriplex Varieties

Kachout¹,

Hamza²,

Bouraoui³

et al. 2013

Not Bot Hort Agrobot Cluj

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This study examined the influence of salt levels on antioxidant activity and content of carotenoids and anthocyanins of the A. hortensis leaves using two varieties: green orach (var. purpurea) and red orach (var. rubra). Seeds of Atriplex were exposed to 0, 90, 180 and 260 mM NaCl for 3 months and seeds were sown in an earthen pot. Overall levels of ascorbate peroxidase (APX) and glutathione reductase (GR) activity were significantly elevated. Salt stress caused a significant decline in tissue concentrations of catalase (CAT) and superoxide dismutase (SOD). However, 90 mM NaCl did not modify these parameters, which remains similar to control values. Activities of APX and CAT were increase whether the shoots of A. hortensis var. purpurea were grown in the presence of 180 mM NaCl. Thus although some indications of oxidative stress accompany exposure of this salt-tolerant Atriplex varieties to salinity, mechanisms appear to exist within its shoot tissue to permit the tolerance of such oxidative stress. High salt concentration in the culture medium provokes oxidative damage in Atriplex leaves and induces a general increase in antioxidant enzyme activity. In particular, NaCl toxicity decreased content of carotenoids. It also decreased the concentration of anthocyanin pigments in leaves of Atriplex. This work therefore provides a starting point towards a better understanding of the role of antioxidant enzyme in the plant response against salt stress.

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“…They further demonstrated that the HRinduced lipid peroxidation could be inhibited by the addition of SOD (1 1). Doke (4) ('02) is an excited state of molecular oxygen which can be generated in a number of ways including the spontaneous dismutation of two 2-radicals, and by the interaction of 02 with the triplet state of photosensitizing compounds such as Chl (7,13). '02 is a very potent inducer of lipid peroxidation and membrane damage (13).…”

mentioning

confidence: 99%

“…Doke (4) ('02) is an excited state of molecular oxygen which can be generated in a number of ways including the spontaneous dismutation of two 2-radicals, and by the interaction of 02 with the triplet state of photosensitizing compounds such as Chl (7,13). '02 is a very potent inducer of lipid peroxidation and membrane damage (13). There has been one brief report that 3-carotene (a singlet oxygen quencher) increased bacterial populations in an incompatible host when coinjected into leaves, suggesting an involvement of '02 in the HR (16).…”

mentioning

confidence: 99%

Effects of Singlet Oxygen Quenchers and pH on the Bacterially Induced Hypersensitive Reaction in Tobacco Suspension Cell Cultures

Salzwedel¹,

Daub²,

Huang³

1989

Plant Physiol.

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Suspension cell cultures of Nicotiana tabacum L. inoculated with the incompatible pathogen Pseudomonas syringae pv pisi undergo a hypersensitive reaction. Addition of the singlet oxygen quencher bixin to cell suspensions had no effect on hypersensitive cell death. Addition of the singlet oxygen quencher 1,4-diazabicyclo octane (DABCO) increased the medium pH and delayed the onset of cell death. This delay was eliminated when cell suspensions were buffered, and could also be induced by increasing medium pH with KOH. Bixin and DABCO also did not suppress the hypersensitive reaction in tobacco leaves. These data do not support a role for singlet oxygen in the hypersensitive reaction. Medium pH, however, appears to be a critical factor in cell suspension cultures.Several reports have suggested that active oxygen-induced lipid peroxidation may play a role in the HR,3 a defense reaction commonly induced by plant pathogens following inoculation of incompatible hosts. Keppler and Novacky (10) reported increases in lipid peroxidation during the HR in inoculated cucumber cotyledons and postulated that this increased lipid peroxidation leads to the typical loss of electrolytes during the HR. They further demonstrated that the HRinduced lipid peroxidation could be inhibited by the addition of SOD (1 1 ('02) is an excited state of molecular oxygen which can be generated in a number of ways including the spontaneous dismutation of two 2-radicals, and by the interaction of 02 with the triplet state of photosensitizing compounds such as Chl (7, 13). '02 is a very potent inducer of lipid peroxidation and membrane damage (13). There has been one brief report that 3-carotene (a singlet oxygen quencher) increased bacterial populations in an incompatible host when coinjected into leaves, suggesting an involvement of '02 in the HR (16). The purpose ofthis study was to further investigate a possible role of '02 in the HR.In this study we investigated the effects oftwo singlet oxygen quenchers, DABCO and the carotenoid carboxylic acid bixin, on the HR induced in leaves and in cell suspension cultures of tobacco following inoculation with the incompatible pathogen Pseudomonas syringae pv pisi. Both ofthese compounds are potent 102 quenchers and have been shown to protect tobacco cell suspension cultures against photosensitizer-generated 102 (3). Suspension cultured cells have been used in previous studies of bacterially induced HR (1,2). In this study, changes occurring in cells undergoing the HR were followed by using the fluorescent vital stain FDA (15, 19). Decreased staining of cells by FDA has been correlated with progress of the HR in cell suspension cultures (9). MATERIALS AND METHODS BacteriaCultures ofPseudomonas syringae pv pisi Young, Dye, and Wilkie were originally obtained from R. N. Goodman, University of Missouri. Cultures were grown for 24 h at 28°C on nutrient agar (Difco) with 0.5% yeast extract and 1.0% dextrose (NYDA) at pH 7.0. Bacteria were suspended in sterile water or 50 mM Mes buffer (pH 6.0) and the c...

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Singlet oxygen and plants

Cited by 313 publications

References 142 publications

Water deficit effect on the accumulation of biomass and artemisinin in annual wormwood(Artemisia annua L., Asteraceae)

Water deficit effect on the accumulation of biomass and artemisinin in annual wormwood(Artemisia annua L., Asteraceae)

Salt-Induced Changes in Antioxidative Enzyme Activities in Shoot Tissues of Two Atriplex Varieties

Effects of Singlet Oxygen Quenchers and pH on the Bacterially Induced Hypersensitive Reaction in Tobacco Suspension Cell Cultures

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