Singlet oxygen-based photoelectrochemical detection of DNA

Antitumor Effect of Photodynamic Therapy/Sonodynamic Therapy/Sono-Photodynamic Therapy of Chlorin e6 and Other Applications

“…Photocurrent response can be detected by dissolved DNA probes labeled with chlorin e6 and erythrosine in the presence of a redox reporter, hydroquinone, according to the findings of Shanmugam. 90 The authors point out that after additional optimization, this method can be used in future applications such as polymerase chain reaction, which is not applicable due to its limitations and can be an effective substitute for colorimetric detection when rapid detection of specific nucleic acid targets is required. In addition, the authors point out that this method can detect specific nucleic acid targets more quickly than colorimetric detection.…”

“…This probe can emit dual-wavelength fluorescence when excited at 410 nm and can determine the amount of OH radicals present in vivo. Photocurrent response can be detected by dissolved DNA probes labeled with chlorin e6 and erythrosine in the presence of a redox reporter, hydroquinone, according to the findings of Shanmugam . The authors point out that after additional optimization, this method can be used in future applications such as polymerase chain reaction, which is not applicable due to its limitations and can be an effective substitute for colorimetric detection when rapid detection of specific nucleic acid targets is required.…”

A Photosensitized Singlet Oxygen (1O2) Toolbox for Bio-Organic Applications: Tailoring 1O2 Generation for DNA and Protein Labelling, Targeting and Biosensing

“…In their work, the group exploits commercially available and known DNA probes bearing a chromophore (type II PSs) and explores the detectability of these conjugates onto a gold electrode in different set-ups: probes directly captured on the gold surface, dissolved in solution and captured on a surface of magnetic beads, functionalized with complementary capture probes. Here, hydroquinone was used as a redox marker, allowing amperometric detection of the target [ 212 ].…”

SARS-CoV-2 detection enabled by a portable and label-free photoelectrochemical genosensor using graphitic carbon nitride and gold nanoparticles

“…The separation between excitation and signal detection can result in unique characteristics such as low background noise, low detection limit, and high sensitivity. Moreover, compared to electrochemiluminescence, PEC-based sensors are low-cost and allow miniaturization [18] using microelectronics devices, such as Arduino [19] , [20] , [21] , which can be built for current measurement and/or light source control via its components and programming script, making it a powerful tool for miniaturized PEC development.…”