Singlet oxygen detection in biological systems: Uses and limitations

Koh, Eugene; Fluhr, Robert

doi:10.1080/15592324.2016.1192742

Cited by 35 publications

(23 citation statements)

References 52 publications

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“…Several spectroscopic and microscopic techniques have been used to detect 1 O 2 formation in photosynthetic organisms both in vitro and in vivo 2 , 18 , 19 . Whereas in vitro 1 O 2 detection affords information on the quantification of 1 O 2 formation, in vivo techniques provide evidence on the spatiotemporal characterization of 1 O 2 within the plant cells.…”

Section: Introductionmentioning

confidence: 99%

Singlet oxygen imaging using fluorescent probe Singlet Oxygen Sensor Green in photosynthetic organisms

Prasad

Sedlářová

Pospı́šil

2018

Sci Rep

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Formation of singlet oxygen (1O2) was reported to accompany light stress in plants, contributing to cell signaling or oxidative damage. So far, Singlet Oxygen Sensor Green (SOSG) has been the only commercialized fluorescent probe for 1O2 imaging though it suffers from several limitations (unequal penetration and photosensitization) that need to be carefully considered to avoid misinterpretation of the analysed data. Herein, we present results of a comprehensive study focused on the appropriateness of SOSG for 1O2 imaging in three model photosynthetic organisms, unicellular cyanobacteria Synechocystis sp. PCC 6803, unicellular green alga Chlamydomonas reinhardtii and higher plant Arabidopsis thaliana. Penetration of SOSG differs in both unicellular organisms; while it is rather convenient for Chlamydomonas it is restricted by the presence of mucoid sheath of Synechocystis, which penetrability might be improved by mild heating. In Arabidopsis, SOSG penetration is limited due to tissue complexity which can be increased by pressure infiltration using a shut syringe. Photosensitization of SOSG and SOSG endoperoxide formed by its interaction with 1O2 might be prevented by illumination of samples by a red light. When measured under controlled conditions given above, SOSG might serve as specific probe for detection of intracellular 1O2 formation in photosynthetic organisms.

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Section: Introductionmentioning

confidence: 99%

Singlet oxygen imaging using fluorescent probe Singlet Oxygen Sensor Green in photosynthetic organisms

Prasad

Sedlářová

Pospı́šil

2018

Sci Rep

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“…(b) Pearson's r correlation matrix of nanolipid formulation LD 50 values in OVCAR-5 cells with their respective photochemical and photophysical properties. Individual plots are represented for LD50 values with the rate of DADB decay (c), rate of SOSG increase (d), rate of photobleaching (e), degree of retention of photoactivity (f), and fluorescence quantum yield (g). The only statistically significant correlation exists between the degree of retention of photoactivity and rate of SOSG increase (h, r 2 = 0.9179, P < 0.05).…”

mentioning

confidence: 99%

Nanolipid Formulations of Benzoporphyrin Derivative: Exploring the Dependence of Nanoconstruct Photophysics and Photochemistry on Their Therapeutic Index in Ovarian Cancer Cells

Obaid

Jin

Bano

et al. 2018

Photochem & Photobiology

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With the rapidly emerging designs and applications of light-activated, photodynamic therapy (PDT)-based nanoconstructs, photonanomedicines (PNMs), an unmet need exists to establish whether conventional methods of photochemical and photophysical characterization of photosensitizers are relevant for evaluating new PNMs in order to intelligently guide their design. As a model system, we build on the clinical formulation of benzoporphyrin derivative (BPD), Visudyne , by developing a panel of nanolipid formulations entrapping new lipidated chemical variants of BPD with differing chemical, photochemical and photophysical properties. These are 16:0 and 20:0 lysophosphocholine-BPD (16:0/20:0 BPD-PC), DSPE-PEG-BPD and BPD-cholesterol. We show that Visudyne was the most phototoxic formulation to OVCAR-5 cells, and the least effective was liposomal DSPE-PEG-BPD. However, these differences did not match their optical, photophysical and photochemical properties, as the static BPD quenching was highest in Visudyne, which also exhibited the lowest generation of singlet oxygen. Furthermore, we establish that OVCAR-5 cell phototoxicity also does not correlate with rates of photosensitizer photobleaching and fluorescence quantum yields in any nanolipid formulations. These findings warrant critical future studies into subcellular targets and molecular mechanisms of phototoxicity of photodynamic nanoconstructs, as more reliable prognostic surrogates for predicting efficacy to appropriately and intelligently guide their design.

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“…In quantitative as well as qualitative determination and detection of NO, 2‐4‐carboxyphenyl‐4,4,5,5‐tetramethylimidazoline‐1‐oxyl‐3‐oxide (cPTIO), a NO scavenger may be utilized to check the effect of NO on plants and cultured cells (D'Alessandro et al ). Apart from this, electron paramagnetic resonance (EPR) spectroscopy may be used for a more sensitive way to detect ROS production (Koh and Fluhr ).…”

Section: Nitric Oxide and Root Developmentmentioning

confidence: 99%

NO and ROS implications in the organization of root system architecture

Prakash

Vishwakarma

Singh

et al. 2020

Physiologia Plantarum

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Over the past decades the role of nitric oxide (NO) and reactive oxygen species (ROS) in signaling and cellular responses to stress has witnessed an exponential trend line. Despite advances in the subject, our knowledge of the role of NO and ROS as regulators of stress and plant growth and their implication in signaling pathways is still partial. The crosstalk between NO and ROS during root formation offers new domains to be explored, as it regulates several plant functions. Previous findings indicate that plants utilize these signaling molecules for regulating physiological responses and development. Depending upon cellular concentration, NO either can stimulate or impede root system architecture (RSA) by modulating enzymes through post-translational modifications. Similarly, the ROS signaling molecule network, in association with other hormonal signaling pathways, control the RSA. The spatial regulation of ROS controls cell growth and ROS determine primary root and act in concert with NO to promote lateral root primordia. NO and ROS are two central messenger molecules which act differentially to upregulate or downregulate the expression of genes pertaining to auxin synthesis and to the configuration of root architecture. The investigation concerning the contribution of donors and inhibitors of NO and ROS can further aid in deciphering their role in root development. With this background, this review provides comprehensive details about the effect and function of NO and ROS in the development of RSA.

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Singlet oxygen detection in biological systems: Uses and limitations

Cited by 35 publications

References 52 publications

Singlet oxygen imaging using fluorescent probe Singlet Oxygen Sensor Green in photosynthetic organisms

Singlet oxygen imaging using fluorescent probe Singlet Oxygen Sensor Green in photosynthetic organisms

Nanolipid Formulations of Benzoporphyrin Derivative: Exploring the Dependence of Nanoconstruct Photophysics and Photochemistry on Their Therapeutic Index in Ovarian Cancer Cells

NO and ROS implications in the organization of root system architecture

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