Singlet oxygen photosensitisation by the fluorescent probe Singlet Oxygen Sensor Green®

Ragàs, Xavier; Jiménez-Banzo, Ana; Sánchez‐García, David; Batllori, Xavier; Nonell, Santi

doi:10.1039/b822776d

Cited by 210 publications

(189 citation statements)

References 22 publications

(27 reference statements)

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“…We used a widely used chemical trapping method for 1 O 2 detection following plasma treatment of fluid and E. coli. SOSGR is relatively stable in acidic pH (plasma causes slightly acidic pH), where it fluoresces bright green upon activation/ trap (3,4,18). Singlet oxygen sensor green reagent (SOSGR) has been successfully applied to animal cells, plant cells, and bacterial cells, such as Staphylococcus aureus (13), and to plasma engineering of 1 O 2 production (31).…”

Section: Discussionmentioning

confidence: 99%

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Nonthermal Dielectric-Barrier Discharge Plasma-Induced Inactivation Involves Oxidative DNA Damage and Membrane Lipid Peroxidation in Escherichia coli

Joshi

Cooper

Yost

et al. 2011

Antimicrob Agents Chemother

442

331

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Oxidative stress leads to membrane lipid peroxidation, which yields products causing variable degrees of detrimental oxidative modifications in cells. Reactive oxygen species (ROS) are the key regulators in this process and induce lipid peroxidation in Escherichia coli. Application of nonthermal (cold) plasma is increasingly used for inactivation of surface contaminants. Recently, we reported a successful application of nonthermal plasma, using a floating-electrode dielectric-barrier discharge (FE-DBD) technique for rapid inactivation of bacterial contaminants in normal atmospheric air (S. G. Joshi et al., Am. J. Infect. Control 38:293-301, 2010). In the present report, we demonstrate that FE-DBD plasma-mediated inactivation involves membrane lipid peroxidation in E. coli. Dose-dependent ROS, such as singlet oxygen and hydrogen peroxide-like species generated during plasma-induced oxidative stress, were responsible for membrane lipid peroxidation, and ROS scavengers, such as ␣-tocopherol (vitamin E), were able to significantly inhibit the extent of lipid peroxidation and oxidative DNA damage. These findings indicate that this is a major mechanism involved in FE-DBD plasma-mediated inactivation of bacteria.Nonthermal (cold) dielectric-barrier discharge (DBD) atmospheric-pressure plasma is widely under investigation for use as an alternative sterilization and disinfection method in the fields of biology and medicine. Most recently, we demonstrated that Escherichia coli, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus in both their planktonic form and in biofilms are rapidly inactivated by nonthermal DBD plasma using a floating-electrode technique (11). Complete inactivation of E. coli was seen in less than 120 s when E. coli was present in its planktonic form, and complete inactivation occurred in about 180 s when it was in the biofilm form, making this technique attractive for sterilization processes. E. coli is one of the most common Gram-negative bacterial contaminants responsible for hospital-acquired infections (HAI) and one of the most widely studied organisms in the laboratory and therefore is a good choice to track various oxidative-stress pathways.A DBD plasma-generating probe is an apparatus that generates microsecond-long, high-voltage-pulsed cold plasma between the primary electrode covered with a quartz surface and the surface of the biological sample, which serves as a second electrode. The high-voltage electrode is completely covered with a dielectric barrier, which makes it safe for sterilization applications, and the nature of the applied microsecond pulses do not elevate the surface temperature above 28°C. In the floating-electrode DBD (FE-DBD) plasma setup, the second electrode (biological sample) is not grounded and remains at a floating potential. Discharge ignites when the powered electrode approaches the surface to be treated at a distance (discharge gap) less than about 3 mm, depending on the form, duration, and polarity of the driving voltage, and it is safe to apply...

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Section: Discussionmentioning

confidence: 99%

“…⅐ and does not show any appreciable response to hydroxyl radical ( ⅐ OH) or superoxide ( ⅐ O 2 Ϫ) (3,4,18). A 1 mM stock solution was freshly prepared in methanol (aliquoted and stored at Ϫ20°C in ambercolored microtubes), and a final working concentration of 1 M (i.e., 1:1,000 dilutions) was used in reaction solution after optimization.…”

Section: Omentioning

confidence: 99%

Nonthermal Dielectric-Barrier Discharge Plasma-Induced Inactivation Involves Oxidative DNA Damage and Membrane Lipid Peroxidation in Escherichia coli

Joshi

Cooper

Yost

et al. 2011

Antimicrob Agents Chemother

442

331

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“…S20-S25, ESI †). 16,33,41 A comparison of the temporal evolution of 1 O 2 formation -as indicated by the increase of the SOSG photoluminescence signal -and the photobleaching kinetics in PTB7-Th (Fig. 5a-c) and PTB7 ( Fig.…”

Section: Nickel(ii) Dibutyldithiocarbamate Suppresses Singlet Oxygen mentioning

confidence: 99%

Suppressing photooxidation of conjugated polymers and their blends with fullerenes through nickel chelates

Salvador

Gasparini

Perea

et al. 2017

Energy Environ. Sci.

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Conjugated polymer semiconductors offer unique advantages over conventional semiconductors but tend to suffer from electro-optic performance roll-off, mainly due to reduced photofastness. Here, we demonstrate that the commodity nickel chelate nickel(II) dibutyldithiocarbamate, Ni(dtc) 2 , effectively inhibits photooxidation across a wide range of prototypical p-conjugated polymer semiconductors and blends. The addition of 2-10 wt% of Ni(dtc) 2 increases the resilience of otherwise quickly photobleaching semiconducting thin films, even in the presence of detrimental, radical forming processing additives. Using electron spin resonance spectroscopy and sensitive oxygen probes, we found that Ni(dtc) 2 acts as a broadband stabilizer that inhibits both the formation of reactive radicals and singlet oxygen. The mechanism of stabilization is of sacrificial nature, but contains non-sacrificial contributions in polymers where singlet oxygen is a key driver of photooxidation. Ultrafast pump-probe spectroscopy reveals quenching of triplet excited states as the central mechanism of non-sacrificial stabilization. When introduced into the active layer of organic photovoltaic devices, Ni(dtc) 2 retards the short circuit current loss in air without affecting the sensitive morphology of bulk heterojunctions and without major sacrifices in semiconductor properties. Antioxidants based on nickel complexes thus constitute functional stabilizers for elucidating degradation mechanisms in organic semiconductors and represent a cost-effective route toward organic electronic appliances with extended longevity. Broader contextOrganic semiconductors based on conjugated polymers bear exceptional opportunities for many disruptive technologies, including light and power generation, sensor technology and electronic circuitry, which could potentially be realized in a sustainable fashion using highly efficient and low-cost approaches. However, conjugated polymers suffer from photo-oxidation-induced performance loss and need to be carefully encapsulated, compromising both applicability and cost benefits. In the present work, we introduce a nickel chelate, nickel(II) dibutyldithiocarbamate, as a universal antioxidant for increasing the longevity of conjugated polymers. We carried out a mechanistic study that quantitatively describes the stabilization of conjugated polymer moieties with technological relevance for OLEDs, OPVs and OFETs. We introduce for the first time a figure of merit (FOM) as a stabilization metric for antioxidants in conjugated polymers. This FOM serves likewise to distinguish between sacrificial and non-sacrificial protective mechanisms. We draw wide ranging conclusions that reflect on how conjugated polymer and polymer:fullerene blends photo-oxidize and how the underlying mechanisms can be significantly suppressed in the presence of nickel chelates. Finally, we demonstrate its beneficial use in a broad range of organic photovoltaic devices.

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“…2B, panel iv). A drawback of SOSG is that it can act as a photosensitizer producing 1 O 2 upon exposure to UV radiation and, to a lesser extent, visible light (Ragàs et al, 2009). We exposed a SOSG solution to the light conditions used in this study (1500 mmol photons m 22 s 21 for 30 min), and we observed that SOSG fluorescence at 525 nm was slightly increased (Supplemental Fig.…”

mentioning

confidence: 99%

Singlet Oxygen-Induced Cell Death in Arabidopsis under High-Light Stress Is Controlled by OXI1 Kinase

Shumbe

Chevalier²,

Légeret³

et al. 2016

Plant Physiol.

113

126

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ORCID ID: 0000-0001-7912-7220 (F.M.). Studies of the singlet oxygen (1 O 2 )-overproducing flu and chlorina1 (ch1) mutants of Arabidopsis (Arabidopsis thaliana) have shown that 1 O 2 -induced changes in gene expression can lead to either programmed cell death (PCD) or acclimation. A transcriptomic analysis of the ch1 mutant has allowed the identification of genes whose expression is specifically affected by each phenomenon. One such gene is OXIDATIVE SIGNAL INDUCIBLE1 (OXI1) encoding an AGC kinase that was noticeably induced by excess light energy and 1 O 2 stress conditions leading to cell death. Photo-induced oxidative damage and cell death were drastically reduced in the OXI1 null mutant (oxi1) and in the double mutant ch1*oxi1 compared with the wild type and the ch1 single mutant, respectively. This occurred without any changes in the production rate of 1 O 2 but was cancelled by exogenous applications of the phytohormone jasmonate. OXI1-mediated 1 O 2 signaling appeared to operate through a different pathway from the previously characterized OXI1-dependent response to pathogens and H 2 O 2 and was found to be independent of the EXECUTER proteins. In high-light-stressed plants, the oxi1 mutation was associated with reduced jasmonate levels and with the up-regulation of genes encoding negative regulators of jasmonate signaling and PCD. Our results show that OXI1 is a new regulator of 1 O 2 -induced PCD, likely acting upstream of jasmonate.

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Singlet oxygen photosensitisation by the fluorescent probe Singlet Oxygen Sensor Green®

Abstract: The fluorescent probe Singlet Oxygen Sensor Green is able to produce singlet oxygen under exposure to UV or visible radiation.

Cited by 210 publications

References 22 publications

Nonthermal Dielectric-Barrier Discharge Plasma-Induced Inactivation Involves Oxidative DNA Damage and Membrane Lipid Peroxidation in Escherichia coli

Nonthermal Dielectric-Barrier Discharge Plasma-Induced Inactivation Involves Oxidative DNA Damage and Membrane Lipid Peroxidation in Escherichia coli

Suppressing photooxidation of conjugated polymers and their blends with fullerenes through nickel chelates

Singlet Oxygen-Induced Cell Death in Arabidopsis under High-Light Stress Is Controlled by OXI1 Kinase

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