Singlet oxygen production in thylakoid membranes during photoinhibition as detected by EPR spectroscopy

Hideg, Éva; Spetea, Cornelia; Vass, Imre

doi:10.1007/bf00029386

Cited by 182 publications

(131 citation statements)

References 41 publications

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“…Chlorophyll photosensitized 1 O 2 production as a result of PI by excess PAR has been demonstrated earlier in isolated membranes and chlorophyll protein complexes [8][9][10][11], as well as in leaves in which protein repair is inhibited [19]. This latter experiment, which was first done in our laboratory using DanePy, is repeated here to serve as a starting point to investigate the usefulness of other 1 O 2 probes under the same conditions.…”

Section: Response To 1 O 2 From Photoinhibitionmentioning

confidence: 86%

“…However, a combination of high solar irradiance with other stress factors (for example drought, extreme temperatures or certain herbicides) may lead to PI (for reviews see Refs [6,7]). Under laboratory conditions, isolated photosynthetic membranes provide excellent model systems for studying the primary reactions of PI including 1 O 2 production [8][9][10][11], but these samples lack important components of the defence system. A good compromise between field and in vitro conditions is to use detached leaves or leaf cuttings, in which the efficient protein repair system of the photosynthetic apparatus is inhibited by the addition of a proteinase inhibitor, and therefore the accumulation of damaged PS II is stimulated [12].…”

Section: Introductionmentioning

confidence: 99%

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A comparative study of fluorescent singlet oxygen probes in plant leaves

Hideg

2008

Open Life Sciences

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Four fluorescent singlet oxygen sensors: DanePy, its oxalate salt, Singlet Oxygen Sensor Green and MVP, were infiltrated into tobacco leaves and tested for toxicity, subcellular localization, light sensitivity and capacity to trap the singlet oxygen produced in photoinhibition. For reference, a broad sensitivity free radical probe, TEMPO-9-AC, was also included. Photochemical yield was approximately 15% and 10% inhibited by Singlet Oxygen Sensor Green and MVP, respectively, but was not significantly affected by the other probes. Under photoinhibitory conditions, brought about by irradiating lincomycin-treated leaves with strong photosynthetically active radiation, DanePy and Singlet Oxygen Sensor Green were responsive. Singlet Oxygen Sensor Green was also reactive to low, non-photoinhibitory light exposure of the leaf, which was not characteristic to the other probes. MVP did not respond to singlet oxygen which can partly be explained by a possible attenuation of its blue emission in the leaf, as shown by the example TEMPO-9-AC. DanePy-oxalate did not respond to photosynthetic singlet oxygen due to lack of its penetration into photosynthetic tissue and hence could be useful in detecting any singlet oxygen which escapes from a chloroplast initiation site. DanePy was localized in the chloroplasts, while Singlet Oxygen Sensor Green was mainly found in the epidermal cells preferentially associated with the nucleus.

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Section: Response To 1 O 2 From Photoinhibitionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

A comparative study of fluorescent singlet oxygen probes in plant leaves

Hideg

2008

Open Life Sciences

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“…Diamagnetic spin traps are used that react with free radicals and form an adduct that can be detected using EPR spectroscopy. For example, this method was used to detect the singlet oxygen in thylakoid membranes under photoinhibitory conditions or UV stress (Hideg et al, 1994(Hideg et al, , 1995. The singlet oxygen and superoxide anion radicals can be also detected and localised using dansyl-based fluorescence sensors such as DanePy or HO-1889-NH (Hideg et al, 2002) as well as a Singlet Oxygen Sensors Green (SOGS) (Flors et al, 2006;Plancot et al, 2013).…”

Section: Detection Of Rosmentioning

confidence: 99%

Reactive oxygen species and plant resistance to fungal pathogens

et al. 2015

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Reactive oxygen species (ROS) have been studied for their role in plant development as well as in plant immunity. ROS were consistently observed to accumulate in the plant after the perception of pathogens and microbes and over the years, ROS were postulated to be an integral part of the defence response of the plant. In this article we will focus on recent findings about ROS involved in the interaction of plants with pathogenic fungi. We will describe the ways to detect ROS, their modes of action and their importance in relation to resistance to fungal pathogens. In addition we include some results from works focussing on the fungal interactor and from studies investigating roots during pathogen attack.

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“…Electron paramagnetic resonance (EPR) spectroscopy has been extensively used to measure light-induced singlet oxygen in isolated chloroplasts (Hideg et al, 1994;Hideg et al, 2000). In that case, the specific cell permeable singlet oxygen trap 2,2,6,6-tetramethylpiperidine (TEMP) after reacting with singlet oxygen was converted to the nitroxide radical 2,2,6,6-tetramethylpiperidinyloxy (TEMPO), which can then be detected by EPR (Lion et al, 1976).…”

Section: Stress-induced Accumulation Of Singlet Oxygen In the Dark Anmentioning

confidence: 99%

Singlet Oxygen Signatures Are Detected Independent of Light or Chloroplasts in Response to Multiple Stresses

et al. 2014

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The production of singlet oxygen is typically associated with inefficient dissipation of photosynthetic energy or can arise from light reactions as a result of accumulation of chlorophyll precursors as observed in fluorescent (flu)-like mutants. Such photodynamic production of singlet oxygen is thought to be involved in stress signaling and programmed cell death. Here we show that transcriptomes of multiple stresses, whether from light or dark treatments, were correlated with the transcriptome of the flu mutant. A core gene set of 118 genes, common to singlet oxygen, biotic and abiotic stresses was defined and confirmed to be activated photodynamically by the photosensitizer Rose Bengal. In addition, induction of the core gene set by abiotic and biotic selected stresses was shown to occur in the dark and in nonphotosynthetic tissue. Furthermore, when subjected to various biotic and abiotic stresses in the dark, the singlet oxygen-specific probe Singlet Oxygen Sensor Green detected rapid production of singlet oxygen in the Arabidopsis (Arabidopsis thaliana) root. Subcellular localization of Singlet Oxygen Sensor Green fluorescence showed its accumulation in mitochondria, peroxisomes, and the nucleus, suggesting several compartments as the possible origins or targets for singlet oxygen. Collectively, the results show that singlet oxygen can be produced by multiple stress pathways and can emanate from compartments other than the chloroplast in a light-independent manner. The results imply that the role of singlet oxygen in plant stress regulation and response is more ubiquitous than previously thought.

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Singlet oxygen production in thylakoid membranes during photoinhibition as detected by EPR spectroscopy

Cited by 182 publications

References 41 publications

A comparative study of fluorescent singlet oxygen probes in plant leaves

A comparative study of fluorescent singlet oxygen probes in plant leaves

Reactive oxygen species and plant resistance to fungal pathogens

Singlet Oxygen Signatures Are Detected Independent of Light or Chloroplasts in Response to Multiple Stresses

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