The aim of this study is to examine the music literacy levels of primary teachers. In line with this objective, it is considered that assessing the music literacy levels of primary teachers according to diverse factors is crucial for enhancing the quality of the education provided. The study employed the relational survey model, which is one of the non-experimental quantitative research methods. The research sample consisted of 337 primary teachers who work in the urban and rural areas of Ağrı, Ardahan, Iğdır, and Kars provinces, located in the Eastern Anatolia region. Data were collected through the “Music Literacy Scale to Preschool and Elementary School Education Department” and a personal information form. The results showed that the music literacy of primary teachers was at an intermediate level. Demographically, the variables of gender, employment province, and the number of semesters involving music education in their undergraduate studies did not make a statistically significant difference. However, the variables of age, seniority, adequacy of music education received in the undergraduate period, having extracurricular music education beyond the undergraduate study, and receiving a music certificate during or after the undergraduate study made significant differences.