Sinomenine alleviates high glucose-induced renal glomerular endothelial hyperpermeability by inhibiting the activation of RhoA/ROCK signaling pathway

Yin, Qingqiao; Xia, Yuanyu; Wang, Guan

doi:10.1016/j.bbrc.2016.06.152

Cited by 33 publications

(24 citation statements)

References 28 publications

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“…However, its exact mechanism of action is not clearly understood. Sinomenine also alleviates high glucose-induced diabetic complications [29,30]. Our studies showed that the effects of sinomenine on hyperalgesia correlate with modulating the expression of the P2X 3 receptor in DRG in a T2DM rat model.…”

Section: Shenqiang Rao and Shuangmei Liu Are Joint First Authorsmentioning

confidence: 55%

The effect of sinomenine in diabetic neuropathic pain mediated by the P2X3 receptor in dorsal root ganglia

Rao

Liu

Zou

et al. 2017

Purinergic Signalling

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Type 2 diabetes mellitus (T2DM) accounts for more than 90% of all cases of diabetes mellitus (DM). Diabetic neuropathic pain (DNP) is a common complication of T2DM. Sinomenine is a natural bioactive component extracted from the Sinomenium acutum and has anti-inflammatory effects. The aim of our study was to investigate the effects of sinomenine on DNP mediated by the P2X receptor in dorsal root ganglia (DRG). The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) in T2DM rats were lower than those of control rats. MWT and TWL in T2DM rats treated with sinomenine were higher compared with those in T2DM rats. The expression levels of the P2X protein and mRNA in T2DM rat DRG were higher compared with those of the control, while those in T2DM rats treated with sinomenine were significantly lower compared with those of the T2DM rats. Sinomenine significantly inhibited P2X agonist ATP-activated currents in HEK293 cells transfected with the P2X receptor. Sinomenine decreased the phosphorylation and activation of P38MAPK in T2DM DRG. Therefore, sinomenine treatment may suppress the up-regulated expression and activation of the P2X receptor and relieve the hyperalgesia potentiated by the activation of P38MAPK in T2DM rats.

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Section: Shenqiang Rao and Shuangmei Liu Are Joint First Authorsmentioning

confidence: 55%

The effect of sinomenine in diabetic neuropathic pain mediated by the P2X3 receptor in dorsal root ganglia

Rao

Liu

Zou

et al. 2017

Purinergic Signalling

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“…31 HG was showed to induce renal glomerular endothelial hyperpermeability by damaging the tight junction between cells. 32 In our study, we cultured GEnCs and then stimulated them with HG or chemerin. Results showed that chemerin/ChemR23 axis could induce inflammation and damage in GEnCs.…”

Section: Serum Chemerin Level Was Closely Related With Renal Functionmentioning

confidence: 99%

Chemerin/ChemR23 axis promotes inflammation of glomerular endothelial cells in diabetic nephropathy

Shang

Wang

Zhang

et al. 2019

J Cellular Molecular Medi

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Diabetic nephropathy (DN) is characterized by inflammation of renal tissue. Glomerular endothelial cells (GEnCs) play an important role in inflammation and protein leakage in urine in DN patients. Chemerin and its receptor ChemR23 are inducers of inflammation. The aim of this study was to investigate the function of chemerin/ChemR23 in GEnCs of DN patients. Immunohistochemical staining and qRT‐PCR were used to measure the expression of chemerin, ChemR23 and inflammatory factors in renal tissues of DN patients. Db/db mice were used as animal model. ChemR23 of DN mice was knocked down by injecting LV3‐shRNA into tail vein. Inflammation, physiological and pathological changes in each group was measured. GEnCs were cultured as an in vitro model to study potential signalling pathways. Results showed that expression of chemerin, ChemR23 and inflammatory factors increased in DN patients and mice. LV3‐shRNA alleviated renal damage and inflammation in DN mice. GEnCs stimulated by glucose showed increased chemerin, ChemR23 and inflammatory factors and decreased endothelial marker CD31. Both LV3‐shRNA and SB203580 (p38 MAPK inhibitor) attenuated chemerin‐induced inflammation and injury in GEnCs. Taken together, chemerin/ChemR23 axis played an important role in endothelial injury and inflammation in DN via the p38 MAPK signalling pathway. Suppression of ChemR23 alleviated DN damage.

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“…Sinomenium acutum is a common traditional Chinese medicine used for the treatment of various diseases, such as rheumatic arthritis, neuralgia, dropsy, and dermatophytosis . Sinomenine is a principal alkaloid isolated from the stem and root of S. acutum , it has the pharmacological actions of arthritis amelioration, anti‐inflammation, immune‐modulating, and immunosuppression . PEG/salt ATPS and a solvent extraction system using ILs as additives has been reported in recent years, but its application in alcohol/salt ATPS has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Salting‐out extraction of sinomenine from Sinomenium acutum by an alcohol/salt aqueous two‐phase system using ionic liquids as additives

Qiao

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND: The aqueous two-phase extraction (ATPE) based on organic solvent and inorganic salt was considered a promising method in the extraction, enrichment, and purification field. Ionic liquids (ILs) used as additives to the aqueous two-phase system (ATPS) can improve the extraction yield. RESULTS: In this study, sevenILs were added to the ethanol/ammonium sulfate ATPS and used for the salting-out extraction (SOE) of sinomenine from Sinomenium acutum. The IL [C 2 OHmim]FeCl 4 was screened as the optimal additive, then the influencing factors IL amount, ethanol concentration, salt concentration, and temperature were investigated. The maximum extraction efficiency of 96.4% was obtained under the following conditions: 23.06 wt% ethanol concentration, 27.28 wt% (NH 4 ) 2 SO 4 concentration, 4.0 wt% [C 2 OHmim]FeCl 4 and 40 ∘ C temperature. Moreover, the components in the SOE system can be recycled after extraction. CONCLUSION: Thus, this ATPS had lower cost and higher extraction ability, and it is feasible to scale up using a small amount of ILs. This system can also be used in the extraction of some other important active compounds from natural plant resources.

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Sinomenine alleviates high glucose-induced renal glomerular endothelial hyperpermeability by inhibiting the activation of RhoA/ROCK signaling pathway

Cited by 33 publications

References 28 publications

The effect of sinomenine in diabetic neuropathic pain mediated by the P2X3 receptor in dorsal root ganglia

The effect of sinomenine in diabetic neuropathic pain mediated by the P2X3 receptor in dorsal root ganglia

Chemerin/ChemR23 axis promotes inflammation of glomerular endothelial cells in diabetic nephropathy

Salting‐out extraction of sinomenine from Sinomenium acutum by an alcohol/salt aqueous two‐phase system using ionic liquids as additives

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