Sinomenine restrains breast cancer cells proliferation, migration and invasion via modulation of miR-29/PDCD-4 axis

Abstract

Sinomenine (Sino) is diffusely applied in heal rheumatoid arthritis and neuralgia. Howbeit, the activities of Sino in breast cancer cells remain confused. The research attempted to probe the anti-tumor function of Sino in breast cancer cells and divulge the feasible molecular mechanism. Sion at the 1-16 lM concentrations was exploited for the exposure of MDA-MB-231 or MCF7 cells, and cell growth, migration, invasion, cell cycle-relevant and apoptosis-correlative factors were estimated. Micro RNA (miR)-29 expression was evaluated via enforcing qRT-PCR, and the actions of miR-29 in MDA-MB-231 cells growth, migration and invasion were appraised after the overexpressed or suppressed vectors transfection. The functions of PDCD-4 in JNK and MEK/ERK pathways were estimated by employing western blot. We found that, Sino exposure impeded cell proliferation, provoked cell apoptosis and barricaded cell migration and invasion in MDA-MB-231 and MCF7 cells. Enhancement of miR-29 was observed in Sino-managed cells, and miR-29 overexpression further potentiated the activities of Sino in MDA-MB-231 cells. Additionally, Sino remarkably enhanced PCDC-4 expression via adjusting miR-29 in MDA-MB-231 cells. Beyond that, overexpressed PCDC-4 obstructed JNK and MEK/ERK pathways in MDA-MB-231 cells. Taken together, the explorations unveiled that Sino restrained MDA-MB-231 cells proliferation, migration, invasion, and provoked apoptosis through modulation of miR-29/PDCD-4 axis. HIGHLIGHT 1. Sino inhibits MDA-MB-231 and MCF7 cells proliferation and provokes apoptosis; 2. Sino restrains MDA-MB-231 and MCF7 cells migration and invasion; 3. Sino ascends miR-29 expression in MDA-MB-231 and MCF7 cells; 4. Sino adjusts cell growth, migration and invasion via modulating miR-29; 5. Sino up-regulates PDCD-4 expression through mediating miR-29; 6. PDCD-4 obstructs JNK and MEK/ERK pathways in MDA-MB-231 cells. ARTICLE HISTORY

Sinomenine Can Inhibit the Growth and Invasion Ability of Retinoblastoma Cell through Regulating PI3K/AKT Signaling Pathway

“…As expected, sinomenine can affect the growth and apoptosis of RB cells in vitro; moreover, sinomenine also lead to decreased migration as well as invasion ability of the RB cells, and the effects of sinomenine were in a dose-dependent manner. These results were consistent with its anti-tumor behaviors in other cancers 9,[12][13][14] . Like any other type of cancer cell, RB cells 7,18) were characterized by the uncontrolled growth and increased migration and invasion ability of the tumor cells, the above observations suggested that sinomenine can inhibit the growth and metathesis of WERI-RB-1 and Y79 cells in vitro, suggesting that sinomenine can exert anti-tumor effects in RB.…”

“…In clinical field, sinomenine has been proved to play protective roles in cardiovascular system, immune system, as well as other tissues and organs 10,11) . So far, sinomenine has been regarded as an anti-tumor reagent for the treatment of many types of cancers, for example, gastric cancer, breast cancer and so on [12][13][14] . However, the roles as well as underlying mechanisms of sinomenine in regulating the progression of RB has not been fully elucidated.…”

miR-29c-3p regulates proliferation and migration in ovarian cancer by targeting KIF4A

“…MiR-29 has been related to types of cancer. miR-29 restrains proliferation, migration, and invasion in breast cancer cells through downregulating PDCD-4 [23]. miR-29 targeting PTEN inhibits proliferation and migration of osteosarcoma cells [24].…”

<p>Sinomenine Inhibits Non-Small Cell Lung Cancer via Downregulation of Hexokinases II-Mediated Aerobic Glycolysis</p>

“…The anti-tumor activity of sinomenine was well studied in gastric, 21,22 breast, 23 prostate, 24 cervical 25 cancer, renal cell carcinoma, 26 and glioma. 27 Inhibition of cell cycle progression, 27 angiogenesis, 26 metastasis, 28 and induction of apoptosis or autophagy are involved in sinomenine-mediated anti-tumor activity.…”