Sintering and biocompatibility of copper-doped hydroxyapatite bioceramics

Bazin, Tiphaine; Magnaudeix, Amandine; Mayet, Richard; Carlès, Pierre; Julien, Isabelle; Demourgues, Alain; Gaudon, Manuel; Champion, Éric

doi:10.1016/j.ceramint.2021.01.225

Cited by 48 publications

(26 citation statements)

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“…The materials whose biological properties are evaluated in this paper are exactly the same as those of our previous paper, which detailed the material aspect in detail, and, in particular, the mechanism of copper incorporation according to the heat treatment [ 7 ]. This part will, therefore, not be detailed again and the reader is invited to refer to our previous paper [ 7 ] (which must be considered the 1st part of this study), our related article [ 20 ] and the recent paper of Bazin et al (2021) [ 17 ]. In summary, the following conclusions should be kept in mind: materials treated at 600 °C and 900 °C easily release copper dopants, unlike materials treated at 1200 °C.…”

Section: Resultsmentioning

confidence: 99%

“…The antibacterial properties of copper probably depend on a balance between the amount of copper required for the lysis of bacteria and the division rate of the bacteria, directly related to the richness in nutrients of the environment. From material point of view, the microstructure will also impact exchanges with the biological environment [ 17 ]. To our knowledge, the importance of considering the culture medium used has never been described in the literature; however, it should be taken into account when making comparisons between different studies from the literature as mentioned in the recent review [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…After synthesis, Cu-doped BCP powders were pre-treated by incubation in distilled water for 48 h at a concentration of 250 mg/L in order to remove CaO traces, which were responsible for deleterious pH increases as reported before [ 7 ]. Before biological evaluations, Cu-doped BCP samples were sterilized with dry heat in an oven at 180 °C for 2 h [ 17 , 18 ].…”

Section: Methodsmentioning

confidence: 99%

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Copper-Doped Biphasic Calcium Phosphate Powders: Dopant Release, Cytotoxicity and Antibacterial Properties

et al. 2021

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Cytotoxicity and antibacterial properties associated with the dopant release of Cu-doped Biphasic Calcium Phosphate (BCP) powders, mainly composed of hydroxyapatite mixed with β-tricalcium phosphate powders, were investigated. Twelve BCP ceramics were synthesized at three different sintering temperatures (600 °C, 900 °C and 1200 °C) and four copper doping rates (x = 0.0, 0.05, 0.10 and 0.20, corresponding to the stoichiometric amount of copper in Ca10Cux(PO4)6(OH)2-2xO2x). Cytotoxicity assessments of Cu-doped BCP powders, using MTT assay with human-Mesenchymal Stem Cells (h-MSCs), indicated no cytotoxicity and the release of less than 12 ppm of copper into the biological medium. The antibacterial activity of the powders was determined against both Gram-positive (methicillin-sensitive (MS) and methicillin resistant (MR) Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. The Cu-doped biomaterials exhibited a strong antibacterial activity against MSSA, MRSA and E. coli, releasing approximatively 2.5 ppm after 24 h, whereas 10 ppm were required to induce an antibacterial effect against P. aeruginosa. This study also demonstrated that the culture medium used during experiments can directly impact the antibacterial effect observed; only 4 ppm of Cu2+ were effective for killing all the bacteria in a 1:500 diluted TS medium, whereas 20 ppm were necessary to achieve the same result in a rich, non-diluted standard marrow cell culture medium.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Copper-Doped Biphasic Calcium Phosphate Powders: Dopant Release, Cytotoxicity and Antibacterial Properties

et al. 2021

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“…Kristalleşme yüzdesi (X C %) değerlerinin hesaplanmasında Eşitlik 4. 'de verilen bağıntı kullanılmıştır [38]: 3,7554 3,7387 3,7608 V (nm) 3 3,5508 3,5154 3,5518…”

Section: Xrd Sonuçlarıunclassified

“…Kalsiyum ortofosfat ailesinin en önemli üyelerinden biri olan hidroksiapatit (HAp), kemiğin yapısına benzerliği, mükemmel biyouyumluluğu ve osteokondüfliği sayesinde diş ve kemik gibi sert dokuların tedavisinde sıklıkla kullanılan bir biyoseramik malzemedir [1][2][3][4][5]. HAp'in kimyasal formülü Ca 10 (PO 4 ) 6 (OH) 2 şeklinde olup, stokiyometrik HAp için Ca:P molar oranı 1,67'dir [6].…”

Section: Introductionunclassified

Synthesis and Characterization of Fe and Ti Doped Biphasic Calcium Phosphates

Ateş

İnce

Acar

et al. 2021

Türk Doğa Ve Fen Dergisi

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Anahtar KelimelerHidroksiapatit, Beta trikalsiyum fosfat (β-TCP), X-ışını kırınımı (XRD), Morfoloji Öz: Bu çalışmada, Ti katkısının Fe esaslı çift fazlı kalsiyum fosfat malzemelerinin yapısal, morfolojik ve termal özellikleri üzerine etkileri araştırılmıştır. X-ışını kırınımı (XRD) analizi, üretilen numunelerin hem hidroksiapatit (HAp) hem de beta trikalsiyum fosfat (β-TCP) fazlarına sahip olduğunu doğrulamaktadır. Ayrıca, Ti katkısındaki artışla β-TCP fazının miktarının arttığı görülmektedir. Fourier dönüşümlü kızılötesi (FTIR) spektroskopisi sonuçları, numunelerdeki karakteristik fonksiyonel grupların varlığını doğrulamaktadır. Ti miktarı morfolojiyi etkilemektedir. Numunelerin ısıl davranışları birbirine benzerdir ve oda sıcaklığından 1000 °C'ye kadar tüm numuneler termal olarak kararlıdırlar. Bu sıcaklık aralığında numunelerdeki kütle kayıpları % 1,63'e eşit veya altındadır.

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Mg‐CS/HA Microscaffolds Display Excellent Biodegradability and Controlled Release of Si and Mg Bioactive Ions to Synergistically Promote Vascularized Bone Regeneration

Wei

Zhang

et al. 2023

Adv Materials Inter

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For bone defect repair, it is critical to utilize biomaterials with pro‐angiogenic properties to enhance osteogenesis. Hydroxyapatite (HA)‐based materials widely used in clinical applications have shown much potential for bone repair. However, their predominant calcium phosphate (CaP) composition and poor biodegradability limit their angiogenic potential and hence osteogenic efficiency of HA‐based materials. Here, a magnesium ion‐doped calcium silicate/HA composite microscaffold (Mg‐CS/HA) is fabricated to enhance angiogenesis and osteogenic efficiency for bone repair. Incorporation of CS improved the biodegradability of the Mg‐CS/HA microscaffold, which could simultaneously release Si and Mg bioactive ions during the early stage of implantation, synergistically enhancing angiogenesis and osteogenic efficiency. In co‐culture systems, the synergistic effects of Si and Mg ions promote the “osteogenesis‐angiogenesis coupling effect.” In vivo, the Mg‐CS/HA microscaffold could significantly promote reconstruction of the vascular network and bone regeneration. This study thus provides a new strategy for coordinated release of bioactive ions to achieve synergistic effects on vascularized bone regeneration by HA‐based bone implant materials.

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Sintering and biocompatibility of copper-doped hydroxyapatite bioceramics

Cited by 48 publications

References 48 publications

Copper-Doped Biphasic Calcium Phosphate Powders: Dopant Release, Cytotoxicity and Antibacterial Properties

Copper-Doped Biphasic Calcium Phosphate Powders: Dopant Release, Cytotoxicity and Antibacterial Properties

Synthesis and Characterization of Fe and Ti Doped Biphasic Calcium Phosphates

Mg‐CS/HA Microscaffolds Display Excellent Biodegradability and Controlled Release of Si and Mg Bioactive Ions to Synergistically Promote Vascularized Bone Regeneration

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