Sintesis reagen imunokimia untuk deteksi okratoksin dengan metode imunokromatografik nanopartikel emas Synthesis of immunochemical reagent for ochratoxin detection using gold nanoparticle immunochromatographic method

Abstract

The quality of Indonesiam coffee andcocoa products has been declined due to the contamination of fungi producing ochratoxin, a serious human mycotoxin. Therefore, development of fast, accurate and simple method for early detection of ochratoxin contamination is required. As a part of research attempted to develop early detection technique of ochratoxin in severall commodities, especially agricultural products, the objective of this study was to produce immunochemical reagent for ochratoxin detectionusing immunoglobulin Y (IgY) based on immunochromatographic method. Results showed that OTA-OVA could be synthesized using active ester method with addition of N-hydroxysucciimide and dicyclocarboimide. The intermediate compound produced showed C=O stretching vibrational band at 1600 cm-1 and CO stretching vibrational band at 1300-1000 cm-1. Antibody-gold nanoparticle conjugate was optimally produced at pH 9 and with antibody dilution of 1:7.5 (v/v). There was 50 nm absorbtion shift in visible absorbtion after the antibody was conjugated with gold nanoparticle. Even though the test strip did not show clear visualization, the cut off of ochratoxin concentrationis obviously determined at 10 ppb. This results suggest thatthe technique could be used to detect ochratoxin contamination.

Advances in Chicken IgY-Based Immunoassays for the Detection of Chemical and Biological Hazards in Food Samples

“…In addition, an AuNP-ICA based on IgY was recently established, showing a cutoff value for OTA of 10 ng mL −1 . 139 In addition to mycotoxins, IgY-based immunoassays have been identified for the detection of other toxins. Finlay et al employed scFv as a biological binder to develop an ic-ELISA for domoic acid (DA) detection.…”