Síntesis verde de nanopartículas de magnetita (NPs-Fe3O4): factores y limitaciones

Valenzuela-Amaro, Hiram Martín; Vázquez-Ortega, Perla Guadalupe; Zazueta-Álvarez, David Enrique; López-Miranda, Javier; Rojas‐Contreras, Juan Antonio

doi:10.22201/ceiich.24485691e.2023.30.69744

Cited by 1 publication

(1 citation statement)

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“…Therefore, the adsorption methods of bioactive molecules into biosensor and nanobiosensor devices can be classified into the following categories: (i) Non-covalent immobilization strategies, based on electrostatic interaction, hydrophobic adsorption, and coordination bond formation between biomarker and the surface of nanomaterials (Figure 2) [86,90]. (ii) Covalent immobilization strategies, involving chemical bonds, chemically activate and modify biorecognition molecules, achieving a stable binding.…”

Section: Biofunctionalization Of Nanostructured Surfaces For Interact...mentioning

confidence: 99%

Emerging Applications of Nanobiosensors in Pathogen Detection in Water and Food

Valenzuela-Amaro,

Aguayo-Acosta,

Meléndez-Sánchez

et al. 2023

Biosensors

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Food and waterborne illnesses are still a major concern in health and food safety areas. Every year, almost 0.42 million and 2.2 million deaths related to food and waterborne illness are reported worldwide, respectively. In foodborne pathogens, bacteria such as Salmonella, Shiga-toxin producer Escherichia coli, Campylobacter, and Listeria monocytogenes are considered to be high-concern pathogens. High-concern waterborne pathogens are Vibrio cholerae, leptospirosis, Schistosoma mansoni, and Schistosima japonicum, among others. Despite the major efforts of food and water quality control to monitor the presence of these pathogens of concern in these kinds of sources, foodborne and waterborne illness occurrence is still high globally. For these reasons, the development of novel and faster pathogen-detection methods applicable to real-time surveillance strategies are required. Methods based on biosensor devices have emerged as novel tools for faster detection of food and water pathogens, in contrast to traditional methods that are usually time-consuming and are unsuitable for large-scale monitoring. Biosensor devices can be summarized as devices that use biochemical reactions with a biorecognition section (isolated enzymes, antibodies, tissues, genetic materials, or aptamers) to detect pathogens. In most cases, biosensors are based on the correlation of electrical, thermal, or optical signals in the presence of pathogen biomarkers. The application of nano and molecular technologies allows the identification of pathogens in a faster and high-sensibility manner, at extremely low-pathogen concentrations. In fact, the integration of gold, silver, iron, and magnetic nanoparticles (NP) in biosensors has demonstrated an improvement in their detection functionality. The present review summarizes the principal application of nanomaterials and biosensor-based devices for the detection of pathogens in food and water samples. Additionally, it highlights the improvement of biosensor devices through nanomaterials. Nanomaterials offer unique advantages for pathogen detection. The nanoscale and high specific surface area allows for more effective interaction with pathogenic agents, enhancing the sensitivity and selectivity of the biosensors. Finally, biosensors’ capability to functionalize with specific molecules such as antibodies or nucleic acids facilitates the specific detection of the target pathogens.

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Section: Biofunctionalization Of Nanostructured Surfaces For Interact...mentioning

confidence: 99%