“…Similarly, application of N-, P-, or K-based fertilizers did not change steviol glycoside levels, but did cause precocious flower budding. ,− Visual symptoms of nutrient deficiency in S. rebaudiana include changes in leaf color to dark green (P, B), yellow (N, K), or pale green (chlorosis; S, Mg), sometimes mottled (K) or with dark necrotic spots (Ca, K, S), and an impairment of overall growth (N, P). These deficiencies reduced steviol glycoside levels within the leaves, with the most severe decreases generally observed in Ca, K, and S deficiencies. ,− On the other hand, addition of 5 ppm B to hydroponically grown plants resulted in 50% higher yields of stevioside ( 37 ) and rebaudioside A ( 38 ). , Addition of Zn may also increase steviol glycoside levels significantly . Studies with in vitro cultures further showed that the addition of 3–5% sucrose could effectively increase steviol glycoside production. , …”