SiO2 Fibers of Two Lengths and Their Effect on Cellular Responses of Macrophage-like Cells

Abstract

The immunoreactivity or/and stress response can be induced by nanomaterials’ different properties, such as size, shape, etc. These effects are, however, not yet fully understood. This study aimed to clarify the effects of SiO2 nanofibers (SiO2NFs) on the cellular responses of THP-1-derived macrophage-like cells. The effects of SiO2NFs with different lengths on reactive oxygen species (ROS) and pro-inflammatory cytokines TNF-α and IL-1β in THP-1 cells were evaluated. From the two tested lengths, it was only the L-SiO2NFs with a length ≈ 44 ± 22 µm that could induce ROS. Compared to this, only S-SiO2NFs with a length ≈ 14 ± 17 µm could enhance TNF-α and IL-1β expression. Our results suggested that L-SiO2NFs disassembled by THP-1 cells produced ROS and that the inflammatory reaction was induced by the uptake of S-SiO2NFs by THP-1 cells. The F-actin staining results indicated that SiO2NFs induced cell motility and phagocytosis. There was no difference in cytotoxicity between L- and S-SiO2NFs. However, our results suggested that the lengths of SiO2NFs induced different cellular responses.

Dendronized Ag/Au Nanomats: Antimicrobial Scaffold for Wound Healing Bandages

“…A high level of interleukin was observed only at a very high concentration of 200 µg mL −1 . [ 56 ] What was also interesting in our obtained results was that the Dend‐AgNPs used to modify nanofibers were less toxic, in the case of fibroblast. This is in agreement with the literature where one can find similar observations in Bonan et al.…”

“…A high level of interleukin was observed only at a very high concentration of 200 μg mL −1 . [56] What was also interesting in our obtained results was that the Dend-AgNPs used to modify nanofibers were less toxic, in the case of fibroblast. This is in agreement with the literature where one can find similar observations in Bonan et al (2019); they showed that cytotoxicity in mouse macrophages treated with Ag/TiO 2 silver nanofibers depended on the concentration of Ag/TiO 2 nanofibers and that cells of the immune response were more sensitive to AgNPs compared to other cell lines.…”

“…This was the case in the studies by Smela et al. (2022), who showed that the SiO 2 nanofibers alone were not cytotoxic and the IL‐1β level was not significantly high, [ 56 ] that TiO 2 nanobelts [ 57 ] and silver nanowires [ 58 ] in contrast can be toxic. They showed that the level of IL‐1β after treatment with SiO 2 nanofibers strictly depends on the length and concentration of the nanofibers.…”